Citation: Hua-Fang Chang, Jun-Qing Wang, Bin Wang, An-Ping Deng. An immunochromatographic assay for rapid and simultaneous detection of levonorgestrel and methylprednisolone in water samples[J]. Chinese Chemical Letters, ;2013, 24(10): 937-940. shu

An immunochromatographic assay for rapid and simultaneous detection of levonorgestrel and methylprednisolone in water samples

Hua-Fang Chang ^a ,
Jun-Qing Wang ^a,b ,
Bin Wang ^a,b ,
An-Ping Deng ^a,b,,

1.
a College of Chemistry, Chemical Engineering & Materials Science, Soochow University, Suzhou 215123, China;
2.
b College of Chemistry, Sichuan University, Chengdu 610064, China

Corresponding author: An-Ping Deng,
Received Date: 17 April 2013
Available Online: 9 May 2013
Fund Project: the Priority Academic Program Development of Jiangsu Higher Education Institutions. (No. 21175097)

Synthetic contraceptive levonorgestrel (LNG) and glucocorticoid methylprednisolone (MP) residues are eventually discarded to environmental water system and function as environmental hormones, displaying potential risk to humans and ecosystems, thus there is an urgent need for fast, sensitive and simultaneous detection of these compounds in water samples. In this study, a competitive immunochromatographic assay (ICA) using colloidal gold-labeled polyclonal antibodies as probes for rapid and simultaneous detection of LNG and MP in water samples was developed. The visual detection limits of LNG and MP in water samples were 10 ng/mL. The detection process could be completed within 10 min. There was no cross-reactivity of the ICA with other seven compounds. The strips could be stored at 4℃ for 10 weeks without significant loss of activity. The assay is a suitable tool for rapid and semiquantitative detection of LNG and MP in water samples on site.
- Immunochromatographic assay,
- Levonorgestrel,
- Methylprednisolone,
- Colloidal gold
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