Citation: Shin-ichi Kawano, Yusuke Inohana, Yuki Hashi, Jin-Ming Lin. Analysis of keto-enol tautomers of curcumin by liquid chromatography/mass spectrometry[J]. Chinese Chemical Letters, ;2013, 24(8): 685-687. shu

Analysis of keto-enol tautomers of curcumin by liquid chromatography/mass spectrometry

1.
a Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Tsinghua University, Beijing 100084, China;
b Shimadzu Global COE for Application & Technical Development, Shimadzu (China) Co.
b Shimadzu Global COE for Application & Technical Development, Shimadzu (China) Co., Ltd., Shanghai 200052, China;
3.
c Global Application Development Center, Shimadzu Corporation, Kyoto 604-8511, Japan

Corresponding author: Jin-Ming Lin,
Received Date: 4 March 2013
Available Online: 23 April 2013

Keto-enol tautomers of curcumin were confirmed by reversed-phase liquid chromatography (RPLC)/ hybrid quadrupole ion trap/time-of-flight mass spectrometry (QIT/TOFMS). Tautomers gave different MS/MS spectra in negative mode. Different mass spectra were also obtained by hydrogen/deuterium exchange LC/MS/MS in positive mode. Our results suggest that enol form is the major form in the solution (water/acetonitrile).
- Keto-enol tautomer,
- Curcumin,
- Liquid chromatography/mass spectrometry
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沈阳化工大学材料科学与工程学院沈阳 110142