Citation: Lei Nie, Guo-Bin Xu, Xiao-Yan Wang, Yun Liu, Peng-Yuan Yang. Coupling microchip electrophoresis with MALDI-TOF-MS based on a freezing technique[J]. Chinese Chemical Letters, ;2013, 24(6): 491-493. shu

Coupling microchip electrophoresis with MALDI-TOF-MS based on a freezing technique

Lei Nie ^a ,
Guo-Bin Xu ^a,b ,
Xiao-Yan Wang ^a ,
Yun Liu ^a ,
Peng-Yuan Yang ^a,b,,

1.
a Department of Chemistry, Fudan University, Shanghai 200433, China;
2.
b Institutes of Biomedical Sciences of Shanghai Medical College, Fudan University, Shanghai 200433, China

Corresponding author: Peng-Yuan Yang,
Received Date: 29 January 2013
Available Online: 31 March 2013

A freezing technique protocol was proposed for coupling microchip electrophoresis withmatrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The microfluidic flow was frozen immediately after electrophoresis on microfluidic chip and the separated analyte molecules were kept in their zone pattern in the electrophoresis. Then, the frozen-chip was lyophilized and sent into TOF-MS instrument as a MALDI target, and the analyte molecules in the microfluidic channels were subjected to analysis by mass spectrometry. This approach could eliminate sample cross-contamination, providing a new interface for microchip electrophoresis and MALDI-MS.
- Microchip electrophoresis,
- MALDI-TOF-MS,
- Peptide analysis,
- Freezing technique
1. [1]
  [1] S.C. Jacobson, J.M. Ramsey, J.P. Landers (Eds.), Handbook of Capillary Electrophoresis, CRC Press, Boca Raton, FL, 1997, p. 827.
2. [2]
  [2] C.S. Effenhauser, A. Manz, H. Becker (Eds.), Microsystem Technology in Chemistry and Life Science, Springer, Berlin, 1998, p. 51.
3. [3]
  [3] S.S. Park, S.I. Cho, M.S. Kim, Integration of on-column immobilized enzyme reactor in microchip electrophoresis, Electrophoresis 24 (2003) 200-206.
4. [4]
  [4] H. Nagata, M. Tabuchi, K. Hirano, High-speed separation of proteins by microchip electrophoresisusingapolyethyleneglycol-coatedplasticchipwithasodiumdodecyl sulfate-linear polyacrylamide solution, Electrophoresis 26 (2005) 2687-2691.
5. [5]
  [5] S. Mouradian, Lab on a chip: application in proteomics, Curr. Opin. Chem. Biol. 6 (2002) 51-56.
6. [6]
  [6] P. Giron, L. Dayon, J.C. Sanchez, Cysteine tagging for MS-based proteomics, Mass Spectrom. Rev. 30 (2011) 366-395.
7. [7]
  [7] N. Liu, L.C. Zhao, C.Y. He, Advances in technologies and biological applications of O-18 labeling strategies in LC-MS based proteomics: an updated review, Curr. Anal. Chem. 8 (2012) 22-34.
8. [8]
  [8] R. Aebersold, M. Mann, Mass spectrometry-based proteomics, Nature 422 (2003) 198-207.
9. [9]
  [9] X.L. Mao, I.K. Chu, B.C. Lin, A sheath-flow nanoelectrospray interface of microchip electrophoresis MS for glycoprotein and glycopeptide analysis, Electrophoresis 27 (2006) 5059-5067.
10. [10]
  [10] W.C. Sung, H. Makamba, S.H. Chen, Chip-based microfluidic devices coupled with electrospray ionization-mass spectrometry, Electrophoresis 26 (2005) 1783-1791.
11. [11]
  [11] Z.J. Meng, S.Z. Qi, S.A. Soper, Interfacing a polymer-based micromachined device to a nano-electrospray ionization Fourier transform ion cyclotron resonance mass spectrometer, Anal. Chem. 73 (2001) 1286-1291.
12. [12]
  [12] S. Ssenyange, J. Taylor, D.J. Harrison, A glassy carbon microfluidic device for electrospray mass spectrometry, Anal. Chem. 76 (2004) 2393-2397.
13. [13]
  [13] M. Schilling, W. Nigge, A. Rudzinski, A new on-chip ESI nozzle for coupling of MS with microfluidic devices, Lab Chip 4 (2004) 220-224.
14. [14]
  [14] S. Arscott, S. Le Gac, C. Druon, A planar on-chip micro-nib interface for nano ESIMS microfluidic applications, J. Micromech. Microeng. 14 (2004) 310-316.
15. [15]
  [15] H.K. Musyimi, J. Guy, D.A. Narcisse, Direct coupling of polymer-based microchip electrophoresis to online MALDI-MS using a rotating ball inlet, Electrophoresis 26 (2005) 4703-4710.
16. [16]
  [16] D.L. DeVoe, C.S. Lee, Microfluidic technologies for MALDI-MS in proteomics, Electrophoresis 27 (2006) 3559-3568.
17. [17]
  [17] K.K. Murray, Coupling matrix-assisted laser desorption/ionization to liquid separations, Mass Spectrom. Rev. 16 (1997) 283-299.
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