Citation: Wei GUO, Zhen Hua SHANG, Yi Nian YU, Liang Mo ZHOU, Zhong Yi SUN, Li Ren MA, Mei Yun CHEN. ENDOTOXIN REMOVAL BY MEMBRANE AFFINITY CHROMATOGRAPHY[J]. Chinese Chemical Letters, ;1995, 6(10): 919-922. shu

ENDOTOXIN REMOVAL BY MEMBRANE AFFINITY CHROMATOGRAPHY

1.
Dallan Institute of Chemical Physics, Chinese Academy of Sclences, P. O. Box 100, Dallan 116012;
2.
Institute of Radiation Medicine, Academy of Military Medical Sciences, P. 0. Box 130, Beijing 100050

Received Date: 28 February 1995

Fund Project: This work was supported by the National Natural Science Foundation of China.

Endotoxin was removed by membrane affinity chromatography.Macropore cellulose membrane was prepared from filter paper by alkaline treatment and chemical crosslinking,and was used as stationary phase for affinity chromatography.The matrix membrane was derived by hexamethylenediamine and activated by glutaraldehyde before histidine was immobilized as affinity ligand.A membrane cartridge containing 40 sheets of affinity membrane was also prepared,which can be used to remove pyrogen from aqueous solutions.
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