Citation: Wu Shiwen, Yang Mengquan, Xiao Youli. Synthetic Biology Studies of Monoterpene Indole Alkaloids[J]. Chinese Journal of Organic Chemistry, ;2018, 38(9): 2243-2258. doi: 10.6023/cjoc201806001 shu

Synthetic Biology Studies of Monoterpene Indole Alkaloids

Wu Shiwen ^a,† ,
Yang Mengquan ^a,b,† ,
Xiao Youli ^a,b,,

a.
CAS Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032
b.
University of Chinese Academy of Sciences, Beijing 100039

Corresponding author: Xiao Youli, ylxiao@sibs.ac.cn
Received Date: 1 June 2018
Revised Date: 4 August 2018
Available Online: 14 September 2018
Fund Project: the Science and Technology Commission of Shanghai Municipality 18ZR1447000the Science and Technology Commission of Shanghai Municipality 15JC1400402Project supported by the Science and Technology Commission of Shanghai Municipality (Nos. 18ZR1447000, 15JC1400402)

Figures(13)

Monoterpene indole alkaloids (MIAs) are a group of important specialized metabolites that exhibit a broad range of pharmacological activities. However, the contents of MIAs in natural plants are extremely low. It could be produced through advanced synthetic chemistry, but the production scale is very limited, and the further modification of the key scaffold is very difficult. With the full elucidation of MIAs biosynthetic pathway and deeper investigation of biotechnology in synthetic biology, the use of synthetic biology strategies combined with chemical semi-synthesis will be an important development trend for the synthesis of these compounds and their derivatives. Elucidation of MIAs biosynthetic pathway by characterizing involved genes is the prerequisite for synthetic biology. Strictosidine, which was formed by the combination of tryptamine and secologanin, is the key intermediate for MIAs biosynthesis. The discovery of biological parts and research advances of synthetic biology of MIAs based on the upstream biosynthetic pathway, the formation of strictosidine, and downstream biosynthetic pathway, production of divergent MIAs derived from strictosidine over the past three decades are described. This review could provide guidance for further elucidation of other MIAs and promotion of synthetic biology for producing MIAs.
- monoterpene indole alkaloid,
- strictosidine,
- biological part,
- biosynthetic pathway,
- synthetic biology
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沈阳化工大学材料科学与工程学院沈阳 110142