Citation: Jiang Yuanyuan, Li Shengying. Catalytic Function and Application of Cytochrome P450 Enzymes in Biosynthesis and Organic Synthesis[J]. Chinese Journal of Organic Chemistry, ;2018, 38(9): 2307-2323. doi: 10.6023/cjoc201805055 shu

Catalytic Function and Application of Cytochrome P450 Enzymes in Biosynthesis and Organic Synthesis

Jiang Yuanyuan ^a,b ,
Li Shengying ^a,b,,

a.
Shandong Provincial Key Laboratory of Synthetic Biology, CAS Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101
b.
University of Chinese Academy of Sciences, Beijing 100049

Corresponding author: Li Shengying, lishengying@qibebt.ac.cn
Received Date: 30 May 2018
Revised Date: 29 June 2018
Available Online: 24 September 2018
Fund Project: the Natural Science Foundation of Shandong Province ZR2017ZB0207the National Natural Science Foundation of China 21472204Project supported by the Natural Science Foundation of Shandong Province (No. ZR2017ZB0207) and the National Natural Science Foundation of China (Nos. 81741115, 21472204)the National Natural Science Foundation of China 81741115

Figures(14)

Cytochrome P450 enzymes are widely distributed in nature, which mainly participate in xenobiotics metabolism and natural product biosynthesis. These enzymes are able to recognize various substrates to produce many useful oxidative products through diverse reaction types. P450 enzymes can catalyze selective oxidation of C-H bonds in their substrates under mild conditions. Therefore, a lot of P450 enzymes have been applied in the production of fine chemicals, drugs and chemical intermediates for quite a long time. With the development of protein engineering, redox partner engineering, substrate engineering, metabolic engineering and synthetic biology, it has become possible to obtain the P450 biocatalysts with the desired properties such as high activity, the substrate specificity of interest, and great selectivity to meet the industrial requirements, through rational design and direct evolution of P450 enzymes. Thus, the application scope of P450 enzymes in biosynthesis and organic synthesis has been expanded greatly. The types of reactions that can be catalyzed by P450 enzymes, and the strategies to broaden the reaction scope and to enhance the catalytic efficiency and selectivity are summarized. Finally, the challenges and prospects in the application of cytochrome P450 enzymes in biosynthesis and organic synthesis are discussed.
- cytochrome P450 enzyme,
- biosynthesis,
- organic synthesis,
- protein engineering,
- substrate engineering,
- metabolic engineering,
- synthetic biology,
- redox partner
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