Citation: Yang Meipan, Su Na, Li Yuxiang, Wang Li, Ma Lifeng, Zhang Yuan, Li Jing, Yang Bingqin, Kang Longli. A New Fluorescent Probe for Fe³⁺ and Its Application to Bioimaging[J]. Chinese Journal of Organic Chemistry, ;2018, 38(3): 636-641. doi: 10.6023/cjoc201709017 shu

A New Fluorescent Probe for Fe³⁺ and Its Application to Bioimaging

Yang Meipan ^a,b,, ,
Su Na ^c ,
Li Yuxiang ^a,b ,
Wang Li ^a,b ,
Ma Lifeng ^a,b ,
Zhang Yuan ^a,b ,
Li Jing ^a,b ,
Yang Bingqin ^c ,
Kang Longli ^a,b,,

a.
Key Laboratory for Molecular Genetic Mechanisms and Intervention Research on High Altitude Disease, Xizang Minzu University, Xianyang 712082
b.
Key Laboratory of High Altitude Environment and Gene Related to Disease, Xizang Minzu University, Xianyang 712082
c.
Key Laboratory for Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, Northwest University, Xi'an 710127

Corresponding author: Yang Meipan, ymp1111@126.com Kang Longli,
Received Date: 11 September 2017
Revised Date: 24 November 2017
Available Online: 1 March 2017
Fund Project: Project supported by the Natural Science Foundation of Xizang (Tibet) Autonomous Region (No. XZ2017ZGR-61) and "The Training Plan for the Youth" Support Program of Xizang Minzu University (No. 17MDQP03)"The Training Plan for the Youth" Support Program of Xizang Minzu University 17MDQP03the Natural Science Foundation of Xizang (Tibet) Autonomous Region XZ2017ZGR-61

Figures(10)

A turn-on probe, which exhibited highly selective and sensitive signaling behaviors toward Fe³⁺ over other common metal ions, has been synthesized and confirmed by ¹H NMR, ¹³C NMR, IR and MS. The addition of Fe³⁺ to the solution of probe induced a remarkable UV-Vis and fluorescence enhancement along with obvious color change detected by the naked eye. The reaction mechanism was also investigated and proposed as that the probe complexed Fe³⁺ via a 1:1 binding mode, and the subsequent complexation caused the ring-opening of the rhodamine spirolactam and thereby the changes of absorbance and fluorescence. These remarkable properties may allow Fe³⁺ to be detected directly in the presence of the other examined competing metal ions. Then, cells experiments showed that the probe could permeate through cell membranes and react to Fe³⁺ within living cells. Additionally, the probe was further used for detecting Fe³⁺ ions in living mouse which exhibited excellent fluorescence imaging.
- fluorescent probe,
- Fe³⁺,
- spectral properties,
- mechanism,
- cells and mouse
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A New Fluorescent Probe for Fe³⁺ and Its Application to Bioimaging