Citation: Zhao Junying, Zhu Yanji, Wang Yanyan, Zhu Jun, Shen Xi. Preparation of Biodegradable Poly (lactic-co-glycolic acid)/Polyethyle-ne Glycol Copolymer and Its Application as a Carrier for Pigment Epithelium-Derived Factor[J]. Chinese Journal of Organic Chemistry, ;2017, 37(1): 203-208. doi: 10.6023/cjoc201608016 shu

Preparation of Biodegradable Poly (lactic-co-glycolic acid)/Polyethyle-ne Glycol Copolymer and Its Application as a Carrier for Pigment Epithelium-Derived Factor

Zhao Junying ^a,† ,
Zhu Yanji ^a,† ,
Wang Yanyan ^b ,
Zhu Jun ^b,*,, ,
Shen Xi ^a,*,,

a.
Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200025
b.
National Engineering Research Center for Nanotechnology, Shanghai 200241

Corresponding author: Zhu Jun, yzjzhu@163.com Shen Xi, carl_shen2005@126.com
Received Date: 22 August 2016
Revised Date: 5 September 2016

Fund Project: the National Natural Science Foundation of China 81470639the Shanghai Natural Science Foundation 14411968400

Figures(8)

A biodegradable poly (lactic-co-glycolic acid)/polyethylene glycol (PLGA-PEG) copolymer molecule was prepared by the simple and green coupling reaction between carboxyl group of poly (lactide-co-glycolide)-carboxylic acid endcap (PLGA-COOH) and amino group of poly (ethylene glycol) amine and carboxylic acid endcap (NH₂-PEG-COOH) in the presence of 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS).The molecular structure of PLGA-PEG copolymer was characterized by ¹H NMR spectroscopy, and the grafting yield of polyethylene glycol (PEG) on poly (lactic-co-glycolic acid)(PLGA) was calculated.Furthermore, pigment epithelium-derived factor (PEDF) was encapsulated in the PLGA-PEG copolymer to prepare a PLGA-PEG/PEDF emulsion for highly efficient inhibition on vascular endothelial growth gene expression (VEGF).The results showed that the obtained PLGA-PEG copolymer was bio-safety and the PLGA-PEG/PEDF emulsion was a good agent for effective inhibition VEGF expression under different conditions.Thus, the PLGA-PEG copolymer can be used as a bio-carrier and the PLGA-PEG/PEDF emulsion can be used as a good angiogenesis inhibitor, which has a potential application for some diseases by anti angiogenesis.
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