Citation: Zhao Junying, Zhu Yanji, Wang Yanyan, Zhu Jun, Shen Xi. Preparation of Biodegradable Poly (lactic-co-glycolic acid)/Polyethyle-ne Glycol Copolymer and Its Application as a Carrier for Pigment Epithelium-Derived Factor[J]. Chinese Journal of Organic Chemistry, ;2017, 37(1): 203-208. doi: 10.6023/cjoc201608016 shu

Preparation of Biodegradable Poly (lactic-co-glycolic acid)/Polyethyle-ne Glycol Copolymer and Its Application as a Carrier for Pigment Epithelium-Derived Factor

Zhao Junying ^a,† ,
Zhu Yanji ^a,† ,
Wang Yanyan ^b ,
Zhu Jun ^b,*,, ,
Shen Xi ^a,*,,

a.
Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200025
b.
National Engineering Research Center for Nanotechnology, Shanghai 200241

Corresponding author: Zhu Jun, yzjzhu@163.com Shen Xi, carl_shen2005@126.com
Received Date: 22 August 2016
Revised Date: 5 September 2016

Fund Project: the National Natural Science Foundation of China 81470639the Shanghai Natural Science Foundation 14411968400

Figures(8)

A biodegradable poly (lactic-co-glycolic acid)/polyethylene glycol (PLGA-PEG) copolymer molecule was prepared by the simple and green coupling reaction between carboxyl group of poly (lactide-co-glycolide)-carboxylic acid endcap (PLGA-COOH) and amino group of poly (ethylene glycol) amine and carboxylic acid endcap (NH₂-PEG-COOH) in the presence of 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS).The molecular structure of PLGA-PEG copolymer was characterized by ¹H NMR spectroscopy, and the grafting yield of polyethylene glycol (PEG) on poly (lactic-co-glycolic acid)(PLGA) was calculated.Furthermore, pigment epithelium-derived factor (PEDF) was encapsulated in the PLGA-PEG copolymer to prepare a PLGA-PEG/PEDF emulsion for highly efficient inhibition on vascular endothelial growth gene expression (VEGF).The results showed that the obtained PLGA-PEG copolymer was bio-safety and the PLGA-PEG/PEDF emulsion was a good agent for effective inhibition VEGF expression under different conditions.Thus, the PLGA-PEG copolymer can be used as a bio-carrier and the PLGA-PEG/PEDF emulsion can be used as a good angiogenesis inhibitor, which has a potential application for some diseases by anti angiogenesis.
1. [1]
  Tomita, M.; Yamada, H.; Adachi, Y.; Cui, Y. Z.; Yamada, E.; Higuchi, A.; Minamino, K.; Suzuki, Y.; Matsumura, M. Stem Cells 2004, 22, 21. doi: 10.1634/stemcells.22-1-21
2. [2]
  Hou, H. Y.; Wang, Y. S.; Xu. J. F.; Wang, Y. C.; Liu, J. P. Curr. Eye Res. 2006, 31, 1051. doi: 10.1080/02713680601100459
3. [3]
  Zhang, Z. X.; Wang, Y. S.; Shi, Y. Y.; Hou, H. Y.; Zhang, C.; Cai, Y.; Dou, G. R.; Yao, L. B.; Li, F. Y. Curr. Eye Res. 2011, 36, 838. doi: 10.3109/02713683.2011.593107
4. [4]
  Barnstablea, C. J.; Tombran-Tinkb, J. Prog. Retinal Eye Res. 2004, 23, 561. doi: 10.1016/j.preteyeres.2004.05.002
5. [5]
  Mori, K. G. P.; Ando, A.; McVey, D.; Wei, L. Invest. Ophthalmol. Vis. Sci. 2002, 43, 2428.
6. [6]
  Zhang, S. X.; Wang, J. J.; Gao, G.; Parke, K.; Ma, J. X. J. Mol. Endocrinol. 2006, 37, 1. doi: 10.1677/jme.1.02008
7. [7]
  Gao, G.; Li, Y.; Gee, S.; Dudley, A.; Fant, J.; Crosson, C.; Ma, J, X. J. Biol. Chem. 2002, 277, 9492. doi: 10.1074/jbc.M108004200
8. [8]
  Yoneki, N.; Takami, T.; Ito, T.; Anzai, R.; Fukuda, K.; Kinoshita, K.; Sonotaki, S.; Murakami, Y. Colloids Surf., A 2015, 469, 66. doi: 10.1016/j.colsurfa.2015.01.011
9. [9]
  Cheng, J. J.; Teply, B. A.; Sherifi, I.; Sung, J.; Luther, G; , Gu, F. X.; Levy-Nissenbaum, E.; Radovic-Moreno, A. F.; Langer, R.; Farokhzad, O. C. Biomaterials 2007, 28, 869. doi: 10.1016/j.biomaterials.2006.09.047
10. [10]
  De la Riva, B.; Sánchez, E.; Hernández, A.; Reyes, R.; Tamimi, F.; López-Cabarcos, E.; Delgado, A.; Évora, C. J. Control. Release 2010, 143, 45. doi: 10.1016/j.jconrel.2009.11.026
11. [11]
  Hao, N. B.; Lü, M. H.; Fan, Y. H.; Cao, Y. L.; Zhang, Z. R.; Yang, S. M. Clin. Dev. Immunol. 2012, 2012, 948098.
12. [12]
  Stellmach, V.; Crawford, S. E.; Zhou, W.; Bouck, N. Proc. Natl. Acad. Sci. U. S. A. 2001, 98, 2593. doi: 10.1073/pnas.031252398
13. [13]
  van der Vlies, A. J.; O'Neil, C. P.; Hasegawa, U.; Hammond, N.; Hubbell, J. A. Bioconjugate Chem. 2010, 21, 653. doi: 10.1021/bc9004443
14. [14]
  Mogensen, C. E. Scand. J. Clin. Lab. Invest. 2009, 21, 77.
15. [15]
  Pisani, E.; Tsapis, N.; Paris, J.; Nicolas, V.; Cattel, L.; Fattal, E. Langmuir 2006, 22, 4397. doi: 10.1021/la0601455
1. [1]
  Lewang Yuan , Yaoyao Peng , Zong-Jie Guan , Yu Fang . 二维共价有机框架作为光催化剂在有机合成中的研究进展. Acta Physico-Chimica Sinica, 2025, 41(8): 100086-. doi: 10.1016/j.actphy.2025.100086
2. [2]
  Lili Jiang , Shaoyu Zheng , Xuejiao Liu , Xiaomin Xie . Copper-Catalyzed Oxidative Coupling Reactions for the Synthesis of Aryl Sulfones: A Fundamental and Exploratory Experiment for Undergraduate Teaching. University Chemistry, 2025, 40(7): 267-276. doi: 10.12461/PKU.DXHX202408004
3. [3]
  Zhongyan Cao , Shengnan Jin , Yuxia Wang , Yiyi Chen , Xianqiang Kong , Yuanqing Xu . Advances in Highly Selective Reactions Involving Phenol Derivatives as Aryl Radical Precursors. University Chemistry, 2025, 40(4): 245-252. doi: 10.12461/PKU.DXHX202405186
4. [4]
  Jiaxun Wu , Mingde Li , Li Dang . The R eaction of Metal Selenium Complexes with Olefins as a Tutorial Case Study for Analyzing Molecular Orbital Interaction Modes. University Chemistry, 2025, 40(3): 108-115. doi: 10.12461/PKU.DXHX202405098
5. [5]
  Lina Guo , Ruizhe Li , Chuang Sun , Xiaoli Luo , Yiqiu Shi , Hong Yuan , Shuxin Ouyang , Tierui Zhang . 层状双金属氢氧化物的层间阴离子对衍生的Ni-Al₂O₃催化剂光热催化CO₂甲烷化反应的影响. Acta Physico-Chimica Sinica, 2025, 41(1): 2309002-. doi: 10.3866/PKU.WHXB202309002
6. [6]
  Dan Liu . 可见光-有机小分子协同催化的不对称自由基反应研究进展. University Chemistry, 2025, 40(6): 118-128. doi: 10.12461/PKU.DXHX202408101
7. [7]
  Chunyang Zheng , Shiyu Liu , Nuo Yi , Hong Shang . The Adventures in the Kingdom of Plant Pigments. University Chemistry, 2024, 39(9): 170-176. doi: 10.3866/PKU.DXHX202308085
8. [8]
  Shitao Fu , Jianming Zhang , Cancan Cao , Zhihui Wang , Chaoran Qin , Jian Zhang , Hui Xiong . Study on the Stability of Purple Cabbage Pigment. University Chemistry, 2024, 39(4): 367-372. doi: 10.3866/PKU.DXHX202401059
9. [9]
  Yi Yang , Xin Zhou , Miaoli Gu , Bei Cheng , Zhen Wu , Jianjun Zhang . Femtosecond transient absorption spectroscopy investigation on ultrafast electron transfer in S-scheme ZnO/CdIn₂S₄ photocatalyst for H₂O₂ production and benzylamine oxidation. Acta Physico-Chimica Sinica, 2025, 41(6): 100064-. doi: 10.1016/j.actphy.2025.100064
10. [10]
  Wentao Lin , Wenfeng Wang , Yaofeng Yuan , Chunfa Xu . Concerted Nucleophilic Aromatic Substitution Reactions. University Chemistry, 2024, 39(6): 226-230. doi: 10.3866/PKU.DXHX202310095
11. [11]
  Heng Zhang . Determination of All Rate Constants in the Enzyme Catalyzed Reactions Based on Michaelis-Menten Mechanism. University Chemistry, 2024, 39(4): 395-400. doi: 10.3866/PKU.DXHX202310047
12. [12]
  Yuting Zhang , Zhiqian Wang . Methods and Case Studies for In-Depth Learning of the Aldol Reaction Based on Its Reversible Nature. University Chemistry, 2024, 39(7): 377-380. doi: 10.3866/PKU.DXHX202311037
13. [13]
  Ruitong Zhang , Zhiqiang Zeng , Xiaoguang Zhang . Improvement of Ethyl Acetate Saponification Reaction and Iodine Clock Reaction Experiments. University Chemistry, 2024, 39(8): 197-203. doi: 10.3866/PKU.DXHX202312004
14. [14]
  Wen Xiao , Fazhan Wang , Yangzhuo Gu , Xi He , Na Fan , Qian Zheng , Shugang Qin , Zhongshan He , Yuquan Wei , Xiangrong Song . PEG400-mediated nanocarriers improve the delivery and therapeutic efficiency of mRNA tumor vaccines. Chinese Chemical Letters, 2024, 35(5): 108755-. doi: 10.1016/j.cclet.2023.108755
15. [15]
  Zhuomin Zhang , Hanbing Huang , Liangqiu Lin , Jingsong Liu , Gongke Li . Course Construction of Instrumental Analysis Experiment: Surface-Enhanced Raman Spectroscopy for Rapid Detection of Edible Pigments. University Chemistry, 2024, 39(2): 133-139. doi: 10.3866/PKU.DXHX202308034
16. [16]
  Yuan Chun , Lijun Yang , Jinyue Yang , Wei Gao . Ideological and Political Design of BZ Oscillatory Reaction Experiment. University Chemistry, 2024, 39(2): 72-76. doi: 10.3866/PKU.DXHX202308072
17. [17]
  Shiyan Cheng , Yonghong Ruan , Lei Gong , Yumei Lin . Research Advances in Friedel-Crafts Alkylation Reaction. University Chemistry, 2024, 39(10): 408-415. doi: 10.12461/PKU.DXHX202403024
18. [18]
  Huijuan Liao , Yulin Xiao , Dong Xue , Mingyu Yang , Jianyang Dong . Synthesis of 1-Benzyl Isoquinoline via the Minisci Reaction. University Chemistry, 2025, 40(7): 294-299. doi: 10.12461/PKU.DXHX202409092
19. [19]
  Yingtong Shi , Guotong Xu , Guizeng Liang , Di Lan , Siyuan Zhang , Yanru Wang , Daohao Li , Guanglei Wu . PEG-VN改性PP隔膜用于高稳定性高效率锂硫电池. Acta Physico-Chimica Sinica, 2025, 41(7): 100082-. doi: 10.1016/j.actphy.2025.100082
20. [20]
  Weina Wang , Lixia Feng , Fengyi Liu , Wenliang Wang . Computational Chemistry Experiments in Facilitating the Study of Organic Reaction Mechanism: A Case Study of Electrophilic Addition of HCl to Asymmetric Alkenes. University Chemistry, 2025, 40(3): 206-214. doi: 10.12461/PKU.DXHX202407022

Get Citation

PDF

XML

Metrics

PDF Downloads(14)
Abstract views(1603)
HTML views(322)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142