Citation: 志洋陈, 雅婷唐, 泽吕, 霄汉孟, 前伟梁, 建国冯. Citronella Oil Nanoemulsion: Formulation, Characterization, Antibacterial Activity, and Cytotoxicity[J]. Acta Physico-Chimica Sinica, ;2022, 38(12): 220505. doi: 10.3866/PKU.WHXB202205053 shu

Citronella Oil Nanoemulsion: Formulation, Characterization, Antibacterial Activity, and Cytotoxicity

School of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, Jiangsu Province, China

Corresponding author: 建国冯, jgfeng@yzu.edu.cn
Received Date: 27 May 2022
Revised Date: 21 June 2022
Accepted Date: 21 June 2022
Available Online: 27 June 2022
Fund Project: the Postgraduate Research & Practice Innovation Program of Jiangsu Province SJCX21_1613

The excessive and unreasonable use of synthetic bactericides in the agricultural field has caused many serious problems, including toxic effects on human health and environmental pollution. Therefore, searching for low toxicity, highly efficient, and no-residue natural bactericides is urgently needed. Plant essential oil has become an emerging and hot topic in the agricultural field because of its excellent bactericidal activity, good biocompatibility, and abundant sources. Citronella oil is a natural plant essential oil with insect repellent, insecticidal, and antibacterial activities, which mainly includes citronellal, geraniol, and citronellol. At present, the major of research on citronella oil focuses on the repellency and control of sanitary pests, but there are relatively few reports on the control of agricultural pathogenic bacteria. In addition, the hydrophobicity and volatility of citronella oil lead to its low bioavailability and hinder its full biological activity. Therefore, constructing a delivery system for improving the hydrophobicity and reducing the volatility of citronella oil is urgently needed. Nanoemulsions have the advantages of fine and uniform droplets, better physical stability, efficient permeation ability, and enhanced bioavailability. Therefore, nanoemulsions are important drug delivery systems for hydrophobic pesticides. In this study, the influences of emulsifier type (hydrophilic-lipophilic balance (HLB)), dosage, and emulsifying time on the formation and stability of citronella oil nanoemulsions were investigated by observing the appearances and microstructures of samples and measuring droplet size, thereby the optimized formula of the citronella oil nanoemulsions was determined. Furthermore, the bioactivity and biosafety of citronella oil nanoemulsions were also investigated. The results showed that nanoemulsions using castor oil polyoxyethylene ethers EL-40 (hydrophilic-lipophilic balance = 13.5) as an emulsifier had the best performance, and the stability of nanoemulsions improved as the emulsifier dosage increased from 3% to 7% (w, mass fraction). In addition, the nanoemulsion prepared through high speed shearing for 3 min was the most stable. The optimal formula for citronella oil nanoemulsions was determined to contain 5% (w) citronella oil, 6% (w) emulsifier (EL-40), and 89% (w) deionized water, upon high speed shearing for 3 min. Then, the inhibitory effect of citronella oil nanoemulsions against the growth of Pantoea ananatis was studied. The concentration for 50% of maximal effect (EC₅₀) of citronella oil nanoemulsions against Pantoea ananatis was 74.85 mg·L⁻¹. The cell viability of L02 cells treated with the citronella oil nanoemulsions (below 100 mg·L⁻¹) was above 83% after 24 h, and the apoptosis rate was 6.93%, indicating that the citronella oil nanoemulsions had low cytotoxicity. This research facilitated the design and fabrication of stable, efficient, and safe agricultural nanoemulsions, and it provides a practical solution for using plant essential oils as agricultural bactericides.
- Citronella oil,
- Nanoemulsion,
- Stability,
- Bactericide,
- Cytotoxicity,
- Environment-friendliness
1. [1]
  Gierer, F.; Vaughan, S.; Slater, M.; Thompson, H. M.; Elmore, J. S.; Girling, R. D. Environ. Pollut. 2019, 249, 236. doi: 10.1016/j.envpol.2019.03.025 doi: 10.1016/j.envpol.2019.03.025
2. [2]
  Sookhtanlou, M.; Allahyari, M. S. Environ. Sci. Pollut. R. 2021, 28 (22), 28168. doi: 10.1007/s11356-021-12502-y doi: 10.1007/s11356-021-12502-y
3. [3]
  Ramakrishnan, B.; Venkateswarlu, K.; Sethunathan, N.; Megharaj, M. Sci. Total Environ. 2019, 654, 177. doi: 10.1016/j.scitotenv.2018.11.041 doi: 10.1016/j.scitotenv.2018.11.041
4. [4]
  Fronza, G.; Toloza, A. C.; Picollo, M. I.; Carbajo, A. E.; Rodriguez, S.; Mougabure-Cueto, G. A. Acta Trop. 2019, 194, 53. doi: 10.1016/j.actatropica.2019.03.021 doi: 10.1016/j.actatropica.2019.03.021
5. [5]
  Feng, J.; Ma, Y.; Chen, Z.; Liu, Q.; Yang, J.; Gao, Y.; Chen, W.; Qian, K.; Yang, W. ACS Sustainable Chem. Eng. 2021, 9 (14), 4988. doi: 10.1021/acssuschemeng.0c08105 doi: 10.1021/acssuschemeng.0c08105
6. [6]
  Sinha, S.; Biswas, D.; Mukherjee, A. J. Ethnopharmacol. 2011, 137 (3), 1521. doi: 10.1016/j.jep.2011.08.046 doi: 10.1016/j.jep.2011.08.046
7. [7]
  He, F.; Wang, W.; Wu, M.; Fang, Y.; Wang, S.; Yang, Y.; Ye, C.; Xiang, F. Ind. Crops Prod. 2020, 153, 112552. doi: 10.1016/j.indcrop.2020.112552 doi: 10.1016/j.indcrop.2020.112552
8. [8]
  Bouyahya, A.; Abrini, J.; Dakka, N.; Bakri, Y. J. Pharm. Anal. 2019, 9 (5), 301. doi: 10.1016/j.jpha.2019.03.001 doi: 10.1016/j.jpha.2019.03.001
9. [9]
  Samba, N.; Aitfella-Lahlou, R.; Nelo, M.; Silva, L.; Coca, R.; Rocha, P.; Lopez Rodilla, J. M. Molecules 2021, 26 (1), 155. doi: 10.3390/molecules26010155 doi: 10.3390/molecules26010155
10. [10]
  Moncada, J.; Tamayo, J. A.; Cardona, C. A. Ind. Crops Prod. 2014, 54, 175. doi: 10.1016/j.indcrop.2014.01.035 doi: 10.1016/j.indcrop.2014.01.035
11. [11]
  Iliou, K.; Kikionis, S.; Petrakis, P. V.; Ioannou, E.; Roussis, V. Pest Manage. Sci. 2019, 75 (8), 2142. doi: 10.1002/ps.5334 doi: 10.1002/ps.5334
12. [12]
  Wan, L.; Li, H.; Huang, C.; Feng, Y.; Chu, G.; Zheng, Y.; Tan, W.; Qin, Y.; Sun, D.; Fang, Y. J. Chem. Thermodyn. 2017, 109, 109. doi: 10.1016/j.jct.2016.12.011 doi: 10.1016/j.jct.2016.12.011
13. [13]
  Rodrigues, K. A. d. F.; Dias, C. N.; do Amaral, F. M. M.; Moraes, D. F. C.; Mouchrek Filho, V. E.; Andrade, E. H. A.; Maia, J. G. S. Pharm. Biol. 2013, 51 (10), 1293. doi: 10.3109/13880209.2013.789536 doi: 10.3109/13880209.2013.789536
14. [14]
  Timung, R.; Barik, C. R.; Purohit, S.; Goud, V. V. Ind. Crops Prod. 2016, 94, 178. doi: 10.1016/j.indcrop.2016.08.021 doi: 10.1016/j.indcrop.2016.08.021
15. [15]
  Motelica, L.; Ficai, D.; Ficai, A.; Trusca, R. -D.; Ilie, C. -I.; Oprea, O. -C.; Andronescu, E. Foods 2020, 9 (12), 121801. doi: 10.3390/foods9121801 doi: 10.3390/foods9121801
16. [16]
  Meng, Y.; Yang, H.; Wang, D.; Ma, Y.; Wang, X.; Blasi, F. Processes 2021, 9 (7), 1199. doi: 10.3390/pr9071199 doi: 10.3390/pr9071199
17. [17]
  Rezaei, A.; Fathi, M.; Jafari, S. M. Food Hydrocolloids 2019, 88, 146. doi: 10.1016/j.foodhyd.2018.10.003 doi: 10.1016/j.foodhyd.2018.10.003
18. [18]
  Rieger, K. A.; Birch, N. P.; Schiffman, J. D. Carbohyd. Polym. 2016, 139, 131. doi: 10.1016/j.carbpol.2015.11.073 doi: 10.1016/j.carbpol.2015.11.073
19. [19]
  Agrawal, N.; Maddikeri, G. L.; Pandit, A. B. Ultrason. Sonochem. 2017, 36, 367. doi: 10.1016/j.ultsonch.2016.11.037 doi: 10.1016/j.ultsonch.2016.11.037
20. [20]
  Sanchez-Gonzalez, L.; Vargas, M.; Gonzalez-Martinez, C.; Chiralt, A.; Chafer, M. Food Eng. Rev. 2011, 3 (1), 1. doi: 10.1007/s12393-010-9031-3 doi: 10.1007/s12393-010-9031-3
21. [21]
  Bouchemal, K.; Briancon, S.; Perrier, E.; Fessi, H. Int. J. Pharm. 2004, 280 (1–2), 241. doi: 10.1016/j.ijpharm.2004.05.016 doi: 10.1016/j.ijpharm.2004.05.016
22. [22]
  Katsouli, M.; Giannou, V.; Tzia, C. Food Funct. 2020, 11 (10), 8878. doi: 10.1039/d0fo01707h doi: 10.1039/d0fo01707h
23. [23]
  Xu, N.; Wu, X.; Zhu, Y.; Miao, J.; Gao, Y.; Cheng, C.; Peng, S.; Zou, L.; McClements, D. J.; Liu, W. Food Chem. 2021, 355, 129508. doi: 10.1016/j.foodchem.2021.129508 doi: 10.1016/j.foodchem.2021.129508
24. [24]
  Feng, J.; Chen, Q.; Wu, X.; Jafari, S. M.; McClements, D. J. Environ. Sci. Pollut. Res. 2018, 25 (22), 21742. doi: 10.1007/s11356-018-2183-z doi: 10.1007/s11356-018-2183-z
25. [25]
  McClements, D. J. Curr. Opin. Colloid Interface Sci. 2017, 28, 7. doi: 10.1016/j.cocis.2016.12.002 doi: 10.1016/j.cocis.2016.12.002
26. [26]
  Li, L. -W.; Chen, X. -Y.; Liu, L. -C.; Yang, Y.; Wu, Y. -J.; Chen, G.; Zhang, Z. -F.; Luo, P. Lwt-Food Sci. Technol. 2021, 140, 110815. doi: 10.1016/j.lwt.2020.110815 doi: 10.1016/j.lwt.2020.110815
27. [27]
  Thapa, R.; Sai, K.; Saha, D.; Kushwaha, D.; Aswal, V. K.; Moulick, R. G.; Bose, S.; Bhattaharya, J. J. Mol. Liq. 2021, 334, 115998. doi: 10.1016/j.molliq.2021.115998 doi: 10.1016/j.molliq.2021.115998
28. [28]
  Zhu, Z.; Wen, Y.; Yi, J.; Cao, Y.; Liu, F.; McClements, D. J. J. Colloid Interface Sci. 2019, 536, 80. doi: 10.1016/j.jcis.2018.10.024 doi: 10.1016/j.jcis.2018.10.024
29. [29]
  Rave, M. C.; Echeverri, J. D.; Salamanca, C. H. J. Food Eng. 2020, 273, 109801. doi: 10.1016/j.jfoodeng.2019.109801 doi: 10.1016/j.jfoodeng.2019.109801
30. [30]
  Liu, Q.; Gao, Y.; Fu, X.; Chen, W.; Yang, J. H.; Chen, Z. Y.; Wang, Z. X.; Zhuansun, X. X.; Feng, J. G.; Chen, Y. Colloids Surf. B 2021, 201, 111626. doi: 10.1016/j.colsurfb.2021.111626 doi: 10.1016/j.colsurfb.2021.111626
31. [31]
  Feng, J.; Chen, W.; Liu, Q.; Chen, Z.; Yang, J.; Yang, W. Pest Manage. Sci. 2020, 76 (12), 4192. doi: 10.1002/ps.5976 doi: 10.1002/ps.5976
32. [32]
  Guan, W. X.; Tang, L. M.; Wang, Y.; Cui, H. J. Agric. Food Chem. 2018, 66 (29), 7568. doi: 10.1021/acs.jafc.8b01388 doi: 10.1021/acs.jafc.8b01388
33. [33]
  Li, J. M.; Chang, J. W.; Saenger, M.; Deering, A. Food Chem. 2017, 232, 191. doi: 10.1016/j.foodchem.2017.03.147 doi: 10.1016/j.foodchem.2017.03.147
34. [34]
  Hong, I. K.; Kim, S. I.; Lee, S. B. J. Ind. Eng. Chem. 2018, 67, 123. doi: 10.1016/j.jiec.2018.06.022 doi: 10.1016/j.jiec.2018.06.022
35. [35]
  Feng, J.; Wang, R.; Chen, Z.; Zhang, S.; Yuan, S.; Cao, H.; Jafari, S. M.; Yang, W. Colloids Surf., A 2020, 596, 124746. doi: 10.1016/j.colsurfa.2020.124746 doi: 10.1016/j.colsurfa.2020.124746
36. [36]
  Rai, V. K.; Mishra, N.; Yadav, K. S.; Yadav, N. P. J. Controlled Release 2018, 270, 203. doi: 10.1016/j.jconrel.2017.11.049 doi: 10.1016/j.jconrel.2017.11.049
37. [37]
  Du, Z.; Wang, C.; Tai, X.; Wang, G.; Liu, X. ACS Sustainable Chem. Eng. 2016, 4 (3), 983. doi: 10.1021/acssuschemeng.5b01058 doi: 10.1021/acssuschemeng.5b01058
38. [38]
  Liang, X.; Wu, J.; Yang, X.; Tu, Z.; Wang, Y. Colloids Surf. Physicochem. Eng. Aspects 2018, 546, 107. doi: 10.1016/j.colsurfa.2018.02.063 doi: 10.1016/j.colsurfa.2018.02.063
39. [39]
  Abbas, S.; Bashari, M.; Akhtar, W.; Li, W. W.; Zhang, X. Ultrason. Sonochem. 2014, 21 (4), 1265. doi: 10.1016/j.ultsonch.2013.12.017 doi: 10.1016/j.ultsonch.2013.12.017
40. [40]
  Fryd, M. M.; Mason, T. G. J. Phys. Chem. Lett. 2010, 1 (23), 3349. doi: 10.1021/jz101365h doi: 10.1021/jz101365h
41. [41]
  Tong, K.; Zhao, C.; Sun, Z.; Sun, D. ACS Sustainable Chem. Eng. 2015, 3 (12), 3299. doi: 10.1021/acssuschemeng.5b00903 doi: 10.1021/acssuschemeng.5b00903
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