Citation: Wang Lulu, Xie Zexin, Zhong Cheng, Tang Yongqiang, Ye Fengming, Wang Liping, Lu Yi. Self-spreadable Octopus-like Electrode Arrays for Long-term Neural Recordings[J]. Acta Physico-Chimica Sinica, ;2020, 36(12): 190903. doi: 10.3866/PKU.WHXB201909035 shu

Self-spreadable Octopus-like Electrode Arrays for Long-term Neural Recordings

Wang Lulu ^1,2,† ,
Xie Zexin ^1,† ,
Zhong Cheng ¹ ,
Tang Yongqiang ^1,2 ,
Ye Fengming ^1,2 ,
Wang Liping ^1,, ,
Lu Yi ^1,,

1.
The Brain Cognition and Brain Disease Institute, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences; Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions, Shenzhen 518055, Guangdong Province, P. R. China
2.
Shenzhen College of Advanced Technology, University of Chinese Academy of Sciences, Shenzhen 518055, Guangdong Province, P. R. China

Corresponding author: Wang Liping, lp.wang@siat.ac.cn Lu Yi, luyi@siat.ac.cn

^†

Received Date: 19 September 2019
Revised Date: 11 November 2019
Accepted Date: 11 November 2019
Available Online: 15 November 2019
Fund Project: the Guangdong Key Lab of Brain Connectome 2017B030301017the Natural Science Foundation of Guangdong Province 2016A030313182the National Natural Science Foundation of China 31871080the Strategic Priority Research Program of the CAS XDBS01030100the Science and Technology Planning Project of Guangdong Province 2018B030331001The project was supported by the National Natural Science Foundation of China (31871080 and 31700921), the Strategic Priority Research Program of the CAS (XDBS01030100), the Youth Innovation Promotion Association of the CAS, the Science and Technology Planning Project of Guangdong Province (2018B030331001), the Natural Science Foundation of Guangdong Province (2016A030313182), and the Guangdong Key Lab of Brain Connectome (2017B030301017)the National Natural Science Foundation of China 31700921

Neural electrodes have been extensively utilized for the investigation of neural functions and the understanding of neuronal circuits because of their high spatial and temporal resolution. However, long-term effective electrophysiological recordings in free-behaving animals still constitute a challenging task, which hinders longitudinal studies on complex brain-processing mechanisms at a functional level. Herein, we demonstrate the feasibility and advantages of using a self-spreadable octopus-like electrode (octrode) array for long-term recordings. The octrode array was fabricated by enwrapping a bundle of eight formvar-coated nickel-chromium microwires with a layer of polyethylene glycol in a custom-made mold. After the electrodeposition of platinum nanoparticles, the microwires at the electrode tip were gathered together and then re-enwrapped with a thin layer of gelatin to maintain their structure and mechanical strength for implantation. Shortly after implantation (within 20 min), the biocompatible gelatin encapsulation swelled and dissolved, causing the self-spreading of the recording channels of the octrode array in the brain. The electrochemical characteristics of the electrode/neural tissue interface were investigated by electrochemical impedance spectroscopy (EIS). Four weeks after implantation, the average impedance of the octrodes (1.26 MΩ at 1 kHz) was significantly lower than that of the conventional tetrodes (1.50 MΩ at 1 kHz, p < 0.05, t-test). Additionally, the octrodes exhibited a better pseudo-capacitive characteristic and a considerably faster ion transfer rate at the electrode interface than the tetrodes. Spontaneous action potentials and local field potentials (LFPs) were also recorded in vivo to investigate the electrophysiological performance of the octrodes. The peak-to-peak spike amplitudes recorded for the octrodes were remarkably larger than those recorded for the tetrodes. The signal quality remained at approximately the same level for the four-week period, while the peak-to-peak spike amplitudes recorded for the tetrodes decreased abruptly. Moreover, the voltage amplitudes recorded by the octrodes at 1–200 Hz were notably larger than those by the tetrodes, suggesting a higher sensitivity in the recording of electrophysiological events. Furthermore, we performed immunochemical analyses on the brain tissues at post-implantation to evaluate the histocompatibility of the electrodes. Tissue responses of the octrodes were alleviated considerably, evidenced by the reduced astroglial intensity and increased neuron density around the implant site as compared to the tetrodes, which may be due to the relatively small size of each decentralized recording channel after self-spreading in vivo. Generally, the fabricated octrodes exhibited a lower electrochemical impedance value at the octrode/neural tissue interface and an increased signal quality during the long-term electrophysiological recording in freely moving mice as compared to the conventional tetrodes. All of these are desirable characteristics in neural circuit dissections in vivo.
- Neural electrode,
- Neural interface,
- Electrode impedance,
- Tissue response,
- Electrophysiological recording
1. [1]
  Spira, M. E.; Hai, A. Nat. Nanotechnol. 2013, 8 (2), 83. doi: 10.1038/nnano.2012.265 doi: 10.1038/nnano.2012.265
2. [2]
  Jun, J. J.; Steinmetz, N. A.; Siegle, J. H.; Denman, D. J.; Bauza, M.; Barbarits, B.; Lee, A. K.; Anastassiou, C. A.; Andrei, A.; Aydin, C.; et al. Nature 2017, 551 (7679), 232. doi: 10.1038/nature24636 doi: 10.1038/nature24636
3. [3]
  Lu, Y.; Zhong, C.; Wang, L.; Wei, P.; He, W.; Huang, K.; Zhang, Y.; Zhan, Y.; Feng, G.; Wang, L. Nat. Commun. 2016, 7, 10962. doi: 10.1038/ncomms10962 doi: 10.1038/ncomms10962
4. [4]
  Gradinaru, V.; Mogri, M.; Thompson, K. R.; Henderson J. M.; Deisseroth. K. Science 2009, 324, 354. doi: 10.1126/science.1167093 doi: 10.1126/science.1167093
5. [5]
  Peca, J.; Feliciano, C.; Ting, J. T.; Wang, W.; Wells, M. F.; Venkatraman, T. N.; Lascola, C. D.; Fu, Z.; Feng, G. Nature 2011, 472 (7344), 437. doi: 10.1038/nature09965 doi: 10.1038/nature09965
6. [6]
  Ferenczi, E. A.; Zalocusky, K. A.; Liston, C.; Grosenick, L.; Warden, M. R.; Amatya, D.; Katovich, K.; Mehta, H.; Patenaude, B.; Ramakrishnan, C.; et al. Science 2016, 351 (6268), aac9698. doi: 10.1126/science.aac9698 doi: 10.1126/science.aac9698
7. [7]
  Wei, P.; Liu, N.; Zhang, Z.; Liu, X.; Tang, Y.; He, X.; Wu, B.; Zhou, Z.; Liu, Y.; Li, J.; et al. Nat. Commun. 2015, 6, 6756. doi: 10.1038/ncomms7756 doi: 10.1038/ncomms7756
8. [8]
  Donoghue, J. P. Neuron 2008, 60 (3), 511. doi: 10.1016/j.neuron.2008.10.037 doi: 10.1016/j.neuron.2008.10.037
9. [9]
  Lu, Z. Y.; Xu, S. W.; Wang, H.; Liu, J. T.; Gao, F.; Song, Y. L.; Xie, J. Y.; Xiao, G. H.; Zhang, Y.; Dai, Y. C.; et al. Acta. Phys. -Chim. Sin. 2020, 36, 1907033. doi: 10.3866/PKU.WHXB201907033
10. [10]
  Polikov, V. S.; Tresco, P. A.; Reichert, W. M. J. Neurosci. Methods 2005, 148 (1), 1. doi: 10.1016/j.jneumeth.2005.08.015 doi: 10.1016/j.jneumeth.2005.08.015
11. [11]
  Williams, J. C.; Rennaker R. L.; Kipke D. R. Brain. Res. Protoc. 1999, 4, 303. doi: 10.1016/s1385-299x(99)00034-3 doi: 10.1016/s1385-299x(99)00034-3
12. [12]
  Neary, J. T.; Kang, Y.; Willoughby, K. A.; Ellis, E. F. J. Neurosci. 2003, 23, 2348. doi: 10.1016/S0006-3223(03)01528-7 doi: 10.1016/S0006-3223(03)01528-7
13. [13]
  Szarowski, D. H.; Andersen, M. D.; Retterer, S.; Spence, A. J.; Isaacson, M.; Craighead, H. G.; Turner, J. N.; Shain, W. Brain Research 2003, 983, 23. doi: 10.1016/s0006-8993(03)03023-3 doi: 10.1016/s0006-8993(03)03023-3
14. [14]
  Lu, Y.; Li, Y.; Pan, J.; Wei, P.; Liu, N.; Wu, B.; Cheng, J.; Lu, C.; Wang, L. Biomaterials 2012, 33 (2), 378. doi: 10.1016/j.biomaterials.2011.09.083 doi: 10.1016/j.biomaterials.2011.09.083
15. [15]
  Chen, R.; Canales, A.; Anikeeva, P. Nat. Rev. Mater. 2017, 2 (2), 16093. doi: 10.1038/natrevmats.2016.93 doi: 10.1038/natrevmats.2016.93
16. [16]
  Ouyang, J. Acta. Phys. -Chim. Sin. 2018, 34 (11), 1211. doi: 10.3866/PKU.WHXB201804095
17. [17]
  Wellman, S. M.; Eles, J. R.; Ludwig, K. A.; Seymour, J. P.; Michelson, N. J.; McFadden, W. E.; Vazquez, A. L.; Kozai, T. D. Y. Adv. Funct. Mater. 2018, 28 (12), 1701269. doi: 10.1002/adfm.201701269 doi: 10.1002/adfm.201701269
18. [18]
  Wang, M.; Mi, G.; Shi, D.; Bassous, N.; Hickey, D.; Webster, T. J. Adv. Funct. Mater. 2018, 28 (12), 1700905. doi: 10.1002/adfm.201700905 doi: 10.1002/adfm.201700905
19. [19]
  Harris, A. R.; Wallace, G. G. Adv. Funct. Mater. 2018, 28 (12), 1700587. doi: 10.1002/adfm.201700587 doi: 10.1002/adfm.201700587
20. [20]
  Lee, H.; Bellamkonda, R. V.; Sun, W.; Levenston, M. E. J. Neural. Eng. 2005, 2 (4), 81. doi: 10.1088/1741-2560/2/4/003 doi: 10.1088/1741-2560/2/4/003
21. [21]
  Gilletti, A.; Muthuswamy, J. J. Neural. Eng. 2006, 3 (3), 189. doi: 10.1088/1741-2560/3/3/001 doi: 10.1088/1741-2560/3/3/001
22. [22]
  Rousche, P. J.; Pellinen, D. S.; Pivin, D. P.; Williams, J. C.; Vetter, R. J.; Kipke, D. R. IEEE. Trans. Biomed. Eng. 2001, 48, 361. doi: 10.1109/10.914800v doi: 10.1109/10.914800v
23. [23]
  Metz, S.; Bertsch, A.; Bertrand, D.; Renaud, P. Biosens Bioelectron 2004, 19 (10), 1309. doi: 10.1016/j.bios.2003.11.021 doi: 10.1016/j.bios.2003.11.021
24. [24]
  Skousen, J. L.; Merriam, S. M.; Srivannavit, O.; Perlin, G.; Wise, K. D.; Tresco, P. A. Prog. Brain. Res. 2011, 194, 167. doi: 10.1016/B978-0-444-53815-4.00009-1 doi: 10.1016/B978-0-444-53815-4.00009-1
25. [25]
  Seymour, J. P.; Kipke, D. R. Biomaterials 2007, 28 (25), 3594. doi: 10.1016/j.biomaterials.2007.03.024 doi: 10.1016/j.biomaterials.2007.03.024
26. [26]
  Liu, J.; Fu, T. M.; Cheng, Z.; Hong, G.; Zhou, T.; Jin, L.; Duvvuri, M.; Jiang, Z.; Kruskal, P.; Xie, C.; et al. Nat. Nanotechnol. 2015, 10 (7), 629. doi: 10.1038/nnano.2015.115 doi: 10.1038/nnano.2015.115
27. [27]
  Xie, C.; Liu, J.; Fu, T. M.; Dai, X.; Zhou, W.; Lieber, C. M. Nat. Mater. 2015, 14 (12), 1286. doi: 10.1038/nmat4427 doi: 10.1038/nmat4427
28. [28]
  Guan, S.; Wang, J.; Gu, X.; Zhao, Y.; Hou, R.; Fan, H.; Zou, L.; Gao, L.; Du, M.; Li, C.; Fang, Y. Sci. Adv. 2019, 5, eaav2842. doi: 10.1126/sciadv.aav2842 doi: 10.1126/sciadv.aav2842
29. [29]
  Zhong, C.; Zhang, Y.; He, W.; Wei, P.; Lu, Y.; Zhu, Y.; Liu, L.; Wang, L. J. Neurosci. Methods. 2014, 222, 218. doi: 10.1016/j.jneumeth.2013.11.013 doi: 10.1016/j.jneumeth.2013.11.013
30. [30]
  Lu, Y.; Li, T.; Zhao, X.; Li, M.; Cao, Y.; Yang, H.; Duan, Y. Y. Biomaterials 2010, 31 (19), 5169. doi: 10.1016/j.biomaterials.2010.03.022 doi: 10.1016/j.biomaterials.2010.03.022
31. [31]
  Zhong, C.; Ke, D.; Wang, L.; Lu, Y.; Wang, L. Electrochem. Commun. 2017, 79, 59. doi: 10.1016/j.elecom.2017.04.015 doi: 10.1016/j.elecom.2017.04.015
32. [32]
  Wang, L.; Zhong, C.; Ke, D.; Ye, F.; Tu, J.; Wang, L.; Lu, Y. Adv. Opt. Mater. 2018, 6, 1800427. doi: 10.1002/adom.201800427 doi: 10.1002/adom.201800427
33. [33]
  Abidian, M. R.; Martin, D. C. Biomaterials 2008, 29 (9), 1273. doi: 10.1016/j.biomaterials.2007.11.022 doi: 10.1016/j.biomaterials.2007.11.022
34. [34]
  Xu, S. D.; Zhuang, Q. C.; Shi, Y. L.; Zhu, Y. B.; Qiu, X. Y.; Sun, Z. Acta Phys. -Chim. Sin. 2011, 27 (10), 2353. doi: 10.3866/PKU.WHXB20111004
35. [35]
  Sun, X. Z.; Huang, B.; Zhang, X.; Zhang, D. C.; Zhang, H. T.; Ma, Y. W. Acta Phys. -Chim. Sin. 2014, 30 (11), 2071. doi: 10.3866/PKU.WHXB201408292
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