Citation: CAO Hong-Yu, GAO Ling-Xing, TANG Qian, SU Jin-Hong, ZHENG Xue-Fang. Mechanism of Oxymyoglobin Oxidation Reaction Induced by Ultraviolet Light[J]. Acta Physico-Chimica Sinica, ;2016, 32(4): 872-878. doi: 10.3866/PKU.WHXB201601046 shu

Mechanism of Oxymyoglobin Oxidation Reaction Induced by Ultraviolet Light

CAO Hong-Yu ^1,3 ,
GAO Ling-Xing ² ,
TANG Qian ^1,3 ,
SU Jin-Hong ² ,
ZHENG Xue-Fang ^1,2,3,,

1.
1. College of Life Science and Biotechnology, Dalian University, Dalian 116622, Liaoning Province, P. R. China;
2.
2. College of Environmental and Chemical Engineering, Dalian University, Dalian 116622, Liaoning Province, P. R. China;
3.
3. Liaoning Key Laboratory of Bio-Organic Chemistry, Dalian University, Dalian 116622, Liaoning Province, P. R. China

Corresponding author: ZHENG Xue-Fang,
Received Date: 9 November 2015
Available Online: 28 December 2015
Fund Project: 国家自然科学基金(21571025, 21271036) (21571025, 21271036)辽宁省教育厅科学技术研究项目(L2013470, L2013471)资助 (L2013470, L2013471)

Myoglobin (Mb) achieves its biological functions such as oxygen transfer and storage in the dark. In this research, we found that UV light irradiation could promote oxidation of ferroporphyrin, which significantly affected the physiological function of oxymyoglobin (MbO₂). The blue shift of the Soret band and decreased intensities of Q-band reduction peaks at 544 and 580 nm observed in UV-Vis absorption spectra revealed that UV light could promote O₂ dissociation, and consequently MbFe(Ⅱ) was oxidized to MbFe(Ⅲ). The effect sequence of wavelength on the strength of this photo-oxidation was 254 nm > 280 nm > 430 nm > 409 nm. CO could inhibit the photo-oxidation process, indicating that the strength of the sixth coordination bond of Fe with a gas molecule influences the degree of photo-oxidation. The H⁺ and OH^- in the solution could also promote the photo-oxidation process. Irradiated by 254 and 280 nm light, free amino acids phenylalanine (Phe), tyrosine (Tyr), and tryptophan (Trp) promoted the light oxidation reaction. Meanwhile, irradiation with 409 and 430 nm light had less influence on the UV-light-induced oxidation reaction under the same conditions. The results illustrated that in photo-induced oxidation of MbO₂, the formation of an excited state of Fe(Ⅱ) with unpaired electrons upon irradiation is crucial process for O₂ dissociation and Fe(Ⅱ) oxidation.
- Oxymyoglobin,
- UV light,
- Free amino acid,
- Photo oxidation,
- Spectrometry
1. [1]
  (1) Wittenberg, J. B.;Wittenberg, B. A. Annu. Rev. Biophys. Biophys. Chem. 1990, 19, 217. doi: 10.1146/annurev.bb.19.060190.001245
2. [2]
  (2) Takahashi, E.; Endoh, H.; Doi, K. Biophys. J. 2000, 78, 3252. doi: 10.1016/S0006-3495(00)76861-5
3. [3]
  (3) Brunori, M. Trends Biochem. Sci. 2001, 26, 209. doi: 10.1016/ S0968-0004(01)01824-2
4. [4]
  (4) Cossins, A.; Berenbrink, M. Nature 2008, 454, 416. doi: 10.1038/454416a
5. [5]
  (5) Kachalova, G. S.; Popov, A. N.; Bartunik, H. D. Science 1999, 284, 473. doi: 10.1126/science.284.5413.473
6. [6]
  (6) Chu, K.; Vojtchovsky, J.; McMahon, B. H.; Sweet, R. M.; Berendzen, J.; Schlichting, I. Nature 2000, 403, 921. doi: 10.1038/35002641
7. [7]
  (7) Bazin, M.; Pierre, J.; Debey, P.; Santus, R. Eur. J. Biochem. 1982, 124, 539. doi: 10.1111/j.1432-1033.1982.tb06627.x
8. [8]
  (8) Sakai, H.; Onuma, H.; Umeyama, M.; Takeoka, S.; Tsuchida, E. Biochemistry 2000, 39, 14595. doi: 10.1021/bi0014204
9. [9]
  (9) Liang, X. Q.; Chen, G. F.; Zhang, X.; Liu, S. L.; Li, G. X. Photo-Chem. Photobiol. B 2008, 90, 53. doi: 10.1016/j.jphotobiol.2007.11.001
10. [10]
  (10) Vorkink, W. P.; Cusanovich, M. A. Photochem. Photobiol. 1974, 19, 205. doi: 10.1111/php.1974.19.issue-3
11. [11]
  (11) Masuda, T.; Minemura, A.; Yamauchi, K.; Kondo, M. J. Radiat Res. 1980, 21, 149. doi: 10.1269/jrr.21.149
12. [12]
  (12) Winterle, J. S.; Einarsdóttir, Ó. Photochem. Photobiol. 2006, 82, 711. doi: 10.1562/2005-09-14-RA-684
13. [13]
  (13) Wittenberg, J. B. Physiol. Rev. 1970, 50, 559.
14. [14]
  (14) Scholander, P. F. Science 1960, 131, 585. doi: 10.1126/ science.131.3400.585
15. [15]
  (15) Nollmann, M.; Etchegoin, P. Spectrochimica Acta Part A 2000, 56, 2817. doi: 10.1016/S1386-1425(00)00384-X
16. [16]
  (16) Etchegoin, P.; Liem, H.; Maher, R. C.; Cohen, F.; Brown, R. J. C.; Milton, M. J. T.; Gallop, J. C. Chem. Phys. Lett. 2003, 367, 223. doi: 10.1016/S0009-2614(02)01705-0
17. [17]
  (17) Franzen, S.; Kiger, L.; Poyart, C.; Martin, J. L. Biophys. J. 2001, 80, 2372. doi: 10.1016/S0006-3495(01)76207-8
18. [18]
  (18) Ma, J. Y.; Zheng, X. F.; Guo, M.; Tang, Q.; Ma, J.; Gao, D. B.; Hu, J. H. Science in China Series B 2008, 51, 414. [马君燕, 郑学仿, 郭明, 唐乾, 马静, 高大彬, 胡皆汉. 中国科学B辑, 2008, 51, 414.] doi: 10.1007/s11426-008-0014-7
19. [19]
  (19) Zhou, H.W.; Cao, H. Y.; Tang, Q.; Ma, J. Y.; Zhang, Y. Y.; Zheng, X. F. Chem. Res. Chin. Univ. 2011, 27 (6), 1060.
20. [20]
  (20) An, L. M.; Cao, H. Y.; Tang, Q.; Zheng, X. F. Chin. J. Inorg. Chem. 2012, 28, 1461. [安良梅, 曹洪玉, 唐乾, 郑学仿. 无机化学学报, 2012, 28, 1461.]
21. [21]
  (21) Liu, Y.W.; Cao, H. Y.; Tang, Q.; Zheng, X. F. Acta Chimica Sinica 2014, 72 (2), 246. [刘艳伟, 曹洪玉, 唐乾, 郑学仿. 化学学报, 2014, 72 (2), 246.] doi: 10.6023/A13101029
22. [22]
  (22) Zhou, H.W.; Cao, H. Y.; Tang, Q.; An, L. M.; Zheng, X. F. Acta Chimica Sinica 2011, 69 (13), 1559. [周华伟, 曹洪玉, 唐乾, 安良梅, 郑学仿. 化学学报, 2011, 69 (13), 1559.]
23. [23]
  (23) Cao, H. Y.; Liu, Y.W.; Zhao, J. M.; Guo, X. J.; Zheng, X. F. Protein & Peptide Letters 2015, 22, 853. doi: 10.2174/0929866522666150707115231
24. [24]
  (24) Zhang, Y. J.; Tang, Q.; Cao, H. Y.; Zheng, X. F. Acta Phys. -Chim. Sin. 2013, 29 (8), 1785. [张玉姣, 唐乾, 曹洪玉, 郑学仿. 物理化学学报, 2013, 29 (8), 1785.] doi: 10.3866/PKU.WHXB201305271
25. [25]
  (25) Huang, H. C.; Jiang, Z. F.; Zhu, H. J. Chemistry 2007, 70, 501. [黄汉昌, 姜招峰, 朱宏吉. 化学通报, 2007, 70, 501.]
1. [1]
  Wei Peng , Baoying Wen , Huamin Li , Yiru Wang , Jianfeng Li . Exploration and Practice on Raman Scattering Spectroscopy Experimental Teaching. University Chemistry, 2024, 39(8): 230-240. doi: 10.3866/PKU.DXHX202312062
2. [2]
  Jianjun LI , Mingjie REN , Lili ZHANG , Lingling ZENG , Huiling WANG , Xiangwu MENG . UV-assisted degradation of tetracycline hydrochloride by MnFe₂O₄@activated carbon activated persulfate. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1869-1880. doi: 10.11862/CJIC.20240187
3. [3]
  Yi Li , Zhaoxiang Cao , Peng Liu , Xia Wu , Dongju Zhang . Revealing the Coloration and Color Change Mechanisms of the Eriochrome Black T Indicator through Computational Chemistry and UV-Visible Absorption Spectroscopy. University Chemistry, 2025, 40(3): 132-139. doi: 10.12461/PKU.DXHX202405154
4. [4]
  Mengyao Shi , Kangle Su , Qingming Lu , Bin Zhang , Xiaowen Xu . Determination of Potassium Content in Tobacco Stem Ash by Flame Atomic Absorption Spectroscopy. University Chemistry, 2024, 39(10): 255-260. doi: 10.12461/PKU.DXHX202404105
5. [5]
  Hong CAI , Jiewen WU , Jingyun LI , Lixian CHEN , Siqi XIAO , Dan LI . Synthesis of a zinc-cobalt bimetallic adenine metal-organic framework for the recognition of sulfur-containing amino acids. Chinese Journal of Inorganic Chemistry, 2025, 41(1): 114-122. doi: 10.11862/CJIC.20240382
6. [6]
  Hui Shi , Shuangyan Huan , Yuzhi Wang . Ideological and Political Design of Potassium Permanganate Oxidation-Reduction Titration Experiment. University Chemistry, 2024, 39(2): 175-180. doi: 10.3866/PKU.DXHX202308042
7. [7]
  Zhuoyan Lv , Yangming Ding , Leilei Kang , Lin Li , Xiao Yan Liu , Aiqin Wang , Tao Zhang . Light-Enhanced Direct Epoxidation of Propylene by Molecular Oxygen over CuO_x/TiO₂ Catalyst. Acta Physico-Chimica Sinica, 2025, 41(4): 100038-. doi: 10.3866/PKU.WHXB202408015
8. [8]
  Zeyu XU , Anlei DANG , Bihua DENG , Xiaoxin ZUO , Yu LU , Ping YANG , Wenzhu YIN . Evaluation of the efficacy of graphene oxide quantum dots as an ovalbumin delivery platform and adjuvant for immune enhancement. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1065-1078. doi: 10.11862/CJIC.20240099
9. [9]
  .
  CCS Chemistry 综述推荐│绿色氧化新思路：光/电催化助力有机物高效升级
  . CCS Chemistry, 2025, 7(10.31635/ccschem.024.202405369): -.
10. [10]
  Xiaofeng Zhu , Bingbing Xiao , Jiaxin Su , Shuai Wang , Qingran Zhang , Jun Wang . Transition Metal Oxides/Chalcogenides for Electrochemical Oxygen Reduction into Hydrogen Peroxides. Acta Physico-Chimica Sinica, 2024, 40(12): 2407005-. doi: 10.3866/PKU.WHXB202407005
11. [11]
  Zhen Yao , Bing Lin , Youping Tian , Tao Li , Wenhui Zhang , Xiongwei Liu , Wude Yang . Visible-Light-Mediated One-Pot Synthesis of Secondary Amines and Mechanistic Exploration. University Chemistry, 2024, 39(5): 201-208. doi: 10.3866/PKU.DXHX202311033
12. [12]
  Yingran Liang , Fei Wang , Jiabao Sun , Hongtao Zheng , Zhenli Zhu . Construction and Application of a New Experimental Device for Determination of Alkaline Metal Elements by Plasma Atomic Emission Spectrometry Based on Solution Cathode Glow Discharge: An Alternative Approach for Fundamental Teaching Experiments in Emission Spectroscopy. University Chemistry, 2024, 39(5): 380-387. doi: 10.3866/PKU.DXHX202312024
13. [13]
  Xin Han , Zhihao Cheng , Jinfeng Zhang , Jie Liu , Cheng Zhong , Wenbin Hu . Design of Amorphous High-Entropy FeCoCrMnBS (Oxy) Hydroxides for Boosting Oxygen Evolution Reaction. Acta Physico-Chimica Sinica, 2025, 41(4): 100033-. doi: 10.3866/PKU.WHXB202404023
14. [14]
  Jiaxin Su , Jiaqi Zhang , Shuming Chai , Yankun Wang , Sibo Wang , Yuanxing Fang . Optimizing Poly(heptazine imide) Photoanodes Using Binary Molten Salt Synthesis for Water Oxidation Reaction. Acta Physico-Chimica Sinica, 2024, 40(12): 2408012-. doi: 10.3866/PKU.WHXB202408012
15. [15]
  Jizhou Liu , Chenbin Ai , Chenrui Hu , Bei Cheng , Jianjun Zhang . 六氯锡酸铵促进钙钛矿太阳能电池界面电子转移及其飞秒瞬态吸收光谱研究. Acta Physico-Chimica Sinica, 2024, 40(11): 2402006-. doi: 10.3866/PKU.WHXB202402006
16. [16]
  Ke Li , Chuang Liu , Jingping Li , Guohong Wang , Kai Wang . 钛酸铋/氮化碳无机有机复合S型异质结纯水光催化产过氧化氢. Acta Physico-Chimica Sinica, 2024, 40(11): 2403009-. doi: 10.3866/PKU.WHXB202403009
17. [17]
  Endong YANG , Haoze TIAN , Ke ZHANG , Yongbing LOU . Efficient oxygen evolution reaction of CuCo₂O₄/NiFe-layered bimetallic hydroxide core-shell nanoflower sphere arrays. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 930-940. doi: 10.11862/CJIC.20230369
18. [18]
  Tianlong Zhang , Rongling Zhang , Hongsheng Tang , Yan Li , Hua Li . Online Monitoring and Mechanistic Analysis of 3,5-diamino-1,2,4-triazole (DAT) Synthesis via Raman Spectroscopy: A Recommendation for a Comprehensive Instrumental Analysis Experiment. University Chemistry, 2024, 39(6): 303-311. doi: 10.3866/PKU.DXHX202312006
19. [19]
  Lu XU , Chengyu ZHANG , Wenjuan JI , Haiying YANG , Yunlong FU . Zinc metal-organic framework with high-density free carboxyl oxygen functionalized pore walls for targeted electrochemical sensing of paracetamol. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 907-918. doi: 10.11862/CJIC.20230431
20. [20]
  Meng Lin , Hanrui Chen , Congcong Xu . Preparation and Study of Photo-Enhanced Electrocatalytic Oxygen Evolution Performance of ZIF-67/Copper(I) Oxide Composite: A Recommended Comprehensive Physical Chemistry Experiment. University Chemistry, 2024, 39(4): 163-168. doi: 10.3866/PKU.DXHX202308117

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(420)
HTML views(61)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142