Citation: CAO Hong-Yu, GAO Ling-Xing, TANG Qian, SU Jin-Hong, ZHENG Xue-Fang. Mechanism of Oxymyoglobin Oxidation Reaction Induced by Ultraviolet Light[J]. Acta Physico-Chimica Sinica, ;2016, 32(4): 872-878. doi: 10.3866/PKU.WHXB201601046 shu

Mechanism of Oxymyoglobin Oxidation Reaction Induced by Ultraviolet Light

CAO Hong-Yu ^1,3 ,
GAO Ling-Xing ² ,
TANG Qian ^1,3 ,
SU Jin-Hong ² ,
ZHENG Xue-Fang ^1,2,3,,

1.
1. College of Life Science and Biotechnology, Dalian University, Dalian 116622, Liaoning Province, P. R. China;
2.
2. College of Environmental and Chemical Engineering, Dalian University, Dalian 116622, Liaoning Province, P. R. China;
3.
3. Liaoning Key Laboratory of Bio-Organic Chemistry, Dalian University, Dalian 116622, Liaoning Province, P. R. China

Corresponding author: ZHENG Xue-Fang,
Received Date: 9 November 2015
Available Online: 28 December 2015
Fund Project: 国家自然科学基金(21571025, 21271036) (21571025, 21271036)辽宁省教育厅科学技术研究项目(L2013470, L2013471)资助 (L2013470, L2013471)

Myoglobin (Mb) achieves its biological functions such as oxygen transfer and storage in the dark. In this research, we found that UV light irradiation could promote oxidation of ferroporphyrin, which significantly affected the physiological function of oxymyoglobin (MbO₂). The blue shift of the Soret band and decreased intensities of Q-band reduction peaks at 544 and 580 nm observed in UV-Vis absorption spectra revealed that UV light could promote O₂ dissociation, and consequently MbFe(Ⅱ) was oxidized to MbFe(Ⅲ). The effect sequence of wavelength on the strength of this photo-oxidation was 254 nm > 280 nm > 430 nm > 409 nm. CO could inhibit the photo-oxidation process, indicating that the strength of the sixth coordination bond of Fe with a gas molecule influences the degree of photo-oxidation. The H⁺ and OH^- in the solution could also promote the photo-oxidation process. Irradiated by 254 and 280 nm light, free amino acids phenylalanine (Phe), tyrosine (Tyr), and tryptophan (Trp) promoted the light oxidation reaction. Meanwhile, irradiation with 409 and 430 nm light had less influence on the UV-light-induced oxidation reaction under the same conditions. The results illustrated that in photo-induced oxidation of MbO₂, the formation of an excited state of Fe(Ⅱ) with unpaired electrons upon irradiation is crucial process for O₂ dissociation and Fe(Ⅱ) oxidation.
- Oxymyoglobin,
- UV light,
- Free amino acid,
- Photo oxidation,
- Spectrometry
1. [1]
  (1) Wittenberg, J. B.;Wittenberg, B. A. Annu. Rev. Biophys. Biophys. Chem. 1990, 19, 217. doi: 10.1146/annurev.bb.19.060190.001245
2. [2]
  (2) Takahashi, E.; Endoh, H.; Doi, K. Biophys. J. 2000, 78, 3252. doi: 10.1016/S0006-3495(00)76861-5
3. [3]
  (3) Brunori, M. Trends Biochem. Sci. 2001, 26, 209. doi: 10.1016/ S0968-0004(01)01824-2
4. [4]
  (4) Cossins, A.; Berenbrink, M. Nature 2008, 454, 416. doi: 10.1038/454416a
5. [5]
  (5) Kachalova, G. S.; Popov, A. N.; Bartunik, H. D. Science 1999, 284, 473. doi: 10.1126/science.284.5413.473
6. [6]
  (6) Chu, K.; Vojtchovsky, J.; McMahon, B. H.; Sweet, R. M.; Berendzen, J.; Schlichting, I. Nature 2000, 403, 921. doi: 10.1038/35002641
7. [7]
  (7) Bazin, M.; Pierre, J.; Debey, P.; Santus, R. Eur. J. Biochem. 1982, 124, 539. doi: 10.1111/j.1432-1033.1982.tb06627.x
8. [8]
  (8) Sakai, H.; Onuma, H.; Umeyama, M.; Takeoka, S.; Tsuchida, E. Biochemistry 2000, 39, 14595. doi: 10.1021/bi0014204
9. [9]
  (9) Liang, X. Q.; Chen, G. F.; Zhang, X.; Liu, S. L.; Li, G. X. Photo-Chem. Photobiol. B 2008, 90, 53. doi: 10.1016/j.jphotobiol.2007.11.001
10. [10]
  (10) Vorkink, W. P.; Cusanovich, M. A. Photochem. Photobiol. 1974, 19, 205. doi: 10.1111/php.1974.19.issue-3
11. [11]
  (11) Masuda, T.; Minemura, A.; Yamauchi, K.; Kondo, M. J. Radiat Res. 1980, 21, 149. doi: 10.1269/jrr.21.149
12. [12]
  (12) Winterle, J. S.; Einarsdóttir, Ó. Photochem. Photobiol. 2006, 82, 711. doi: 10.1562/2005-09-14-RA-684
13. [13]
  (13) Wittenberg, J. B. Physiol. Rev. 1970, 50, 559.
14. [14]
  (14) Scholander, P. F. Science 1960, 131, 585. doi: 10.1126/ science.131.3400.585
15. [15]
  (15) Nollmann, M.; Etchegoin, P. Spectrochimica Acta Part A 2000, 56, 2817. doi: 10.1016/S1386-1425(00)00384-X
16. [16]
  (16) Etchegoin, P.; Liem, H.; Maher, R. C.; Cohen, F.; Brown, R. J. C.; Milton, M. J. T.; Gallop, J. C. Chem. Phys. Lett. 2003, 367, 223. doi: 10.1016/S0009-2614(02)01705-0
17. [17]
  (17) Franzen, S.; Kiger, L.; Poyart, C.; Martin, J. L. Biophys. J. 2001, 80, 2372. doi: 10.1016/S0006-3495(01)76207-8
18. [18]
  (18) Ma, J. Y.; Zheng, X. F.; Guo, M.; Tang, Q.; Ma, J.; Gao, D. B.; Hu, J. H. Science in China Series B 2008, 51, 414. [马君燕, 郑学仿, 郭明, 唐乾, 马静, 高大彬, 胡皆汉. 中国科学B辑, 2008, 51, 414.] doi: 10.1007/s11426-008-0014-7
19. [19]
  (19) Zhou, H.W.; Cao, H. Y.; Tang, Q.; Ma, J. Y.; Zhang, Y. Y.; Zheng, X. F. Chem. Res. Chin. Univ. 2011, 27 (6), 1060.
20. [20]
  (20) An, L. M.; Cao, H. Y.; Tang, Q.; Zheng, X. F. Chin. J. Inorg. Chem. 2012, 28, 1461. [安良梅, 曹洪玉, 唐乾, 郑学仿. 无机化学学报, 2012, 28, 1461.]
21. [21]
  (21) Liu, Y.W.; Cao, H. Y.; Tang, Q.; Zheng, X. F. Acta Chimica Sinica 2014, 72 (2), 246. [刘艳伟, 曹洪玉, 唐乾, 郑学仿. 化学学报, 2014, 72 (2), 246.] doi: 10.6023/A13101029
22. [22]
  (22) Zhou, H.W.; Cao, H. Y.; Tang, Q.; An, L. M.; Zheng, X. F. Acta Chimica Sinica 2011, 69 (13), 1559. [周华伟, 曹洪玉, 唐乾, 安良梅, 郑学仿. 化学学报, 2011, 69 (13), 1559.]
23. [23]
  (23) Cao, H. Y.; Liu, Y.W.; Zhao, J. M.; Guo, X. J.; Zheng, X. F. Protein & Peptide Letters 2015, 22, 853. doi: 10.2174/0929866522666150707115231
24. [24]
  (24) Zhang, Y. J.; Tang, Q.; Cao, H. Y.; Zheng, X. F. Acta Phys. -Chim. Sin. 2013, 29 (8), 1785. [张玉姣, 唐乾, 曹洪玉, 郑学仿. 物理化学学报, 2013, 29 (8), 1785.] doi: 10.3866/PKU.WHXB201305271
25. [25]
  (25) Huang, H. C.; Jiang, Z. F.; Zhu, H. J. Chemistry 2007, 70, 501. [黄汉昌, 姜招峰, 朱宏吉. 化学通报, 2007, 70, 501.]
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