Citation: HU Li-Mei, LIN Cun-Guo, WANG Li, YUAN Shi-Ling. Lysozyme Protein Adsorbed on Antifouling Polymer Film Surface[J]. Acta Physico-Chimica Sinica, ;2014, 30(11): 2149-2156. doi: 10.3866/PKU.WHXB201409021 shu

Lysozyme Protein Adsorbed on Antifouling Polymer Film Surface

HU Li-Mei ^1,2 ,
LIN Cun-Guo ² ,
WANG Li ² ,
YUAN Shi-Ling ^1,

1.
1. Key Laboratory of Colloid and Interface Chemistry, Shandong University, Jinan 250100, P. R. China;
2. State Key Laboratory for Marine Corrosion and Protection, Luoyang Ship Material Research Institute LSMRI, Qingdao 266101, Shandong Province, P. R. China

Received Date: 31 July 2014
Available Online: 2 September 2014
Fund Project: 国家重点基础研究发展规划项目(973) (2014CB643305)资助 (973) (2014CB643305)

Molecular dynamics simulations were used to compare the adsorption behavior of lysozyme on two typical antifouling polymer materials: poly(ethylene) glycol (PEG) and poly(dimethylsiloxane) (PDMS). The influence of the surface properties of the polymer films on protein adsorption is discussed at the microscale. Based on the interactions, energy changes between the protein and polymer films, and dynamical behavior of the hydration molecules near the polymer film, the reasons why the PEG antifouling coating has a better antifouling effect than the PDMS surface were determined as follows. (1) The lower binding energy between the protein and the PEG coating than between the protein and the PDMS coating makes the protein adsorb weaker on the PEG coating than on the PDMS coating. (2) The protein would adsorb on the film surface when overcoming the energy barrier caused by the hydration layer. Molecular water adsorbs on the PEG surface stronger than on the PDMS surface, and is difficult to desorb. Therefore, the protein needs to overcome a higher energy barrier to adsorb to the PEG surface than to the PDMS surface, and thus it is more difficult for protein to absorb on the PEG surface than on the PDMS surface.
- Poly(ethylene) glycol,
- Poly(dimethylsiloxane),
- Antifouling material,
- Molecular dynamics
1. [1]
  
  (1) Ostuni, E.; Chapman, R. G.; Holmlin, R. K.; Takayama, S.; Whitesides, G. M. Langmuir 2001, 17, 5605. doi: 10.1021/la010384m
2. [2]
  (2) Shen, M. C.; Martinson, L.;Wagner, M. S.; Castner, D. G.; Ratner, B. D.; Horbett, T. A. J. Biomater. Sci. Polym. Ed. 2002, 13, 367. doi: 10.1163/156856202320253910
3. [3]
  (3) Chambers, L. D.; Stokes, K. R.;Walsh, F. C.;Wood, R. J. Surface and Coatings Technology 2006, 201 (6), 364.
4. [4]
  (4) Zheng, J.; Li, L.; Chen, S.; Jiang, S. Langmuir 2004, 20 (20), 8931. doi: 10.1021/la036345n
5. [5]
  (5) Cedervall, T.; Lynch, I.; Foy, M.; Berggård, T.; Donnelly, S. C.; Cagney, G.; Dawson, K. A. Angewandte Chemie International Edition 2007, 46 (30), 5754.
6. [6]
  (6) Aggarwal, P.; Hall, J. B.; McLeland, C. B.; Dobrovolskaia, M. A.; McNeil, S. E. Advanced Drug Delivery Reviews 2009, 61 (6), 428. doi: 10.1016/j.addr.2009.03.009
7. [7]
  (7) Kitano, H.; Sudo, K.; Ichikawa, K.; Ide, M.; Ishihara, K. The Journal of Physical Chemistry B 2000, 104 (47), 11425. doi: 10.1021/jp000429c
8. [8]
  (8) Zheng, H. R.;Wang, X.W.; Lin, X. H.; Geng, Q.; Chen, X.; Dai,W. X.;Wang, X. X. Acta Phys. -Chim. Sin. 2012, 28, 1764. [郑华荣, 王晓韡, 林霞晖, 耿强, 陈旬, 戴文新, 王绪绪. 物理化学学报, 2012, 28, 1764.] doi: 10.3866/PKU.WHXB201205112
9. [9]
  (9) Lüsse, S.; Arnold, K. Macromolecules 1996, 29 (12), 4251. doi: 10.1021/ma9508616
10. [10]
  (10) Wang, Y. Q.;Wang, T.; Su, Y. L.; Peng, F. B.;Wu, H.; Jiang, Z. Y. Langmuir 2005, 21 (25), 11856. doi: 10.1021/la052052d
11. [11]
  (11) Zhao,W.; Su, Y.; Li, C.; Shi, Q.; Ning, X.; Jiang, Z. Journal of Membrane Science 2008, 318 (1), 405.
12. [12]
  (12) Chen, H.; Yuan, L.; Song,W.;Wu, Z.; Li, D. Progress in Polymer Science 2008, 33 (11), 1059. doi: 10.1016/j.progpolymsci.2008.07.006
13. [13]
  (13) Michalkova, A.; Tulyani, S.; Beals, J.; Leszczynski, J. Journal of Molecular Modeling 2012, 18 (1), 239. doi: 10.1007/s00894-011-1058-8
14. [14]
  (14) Shen, J.W.;Wu, T.;Wang, Q.; Pan, H. H. Biomaterials 2008, 29 (5), 513. doi: 10.1016/j.biomaterials.2007.10.016
15. [15]
  (15) Bai, S.; Li, H.; Zhang, L. Acta Phys. -Chim. Sin. 2013, 29, 849. [白姝, 李浩, 张麟. 物理化学学报, 2013, 29, 849.] doi: 10.3866/PKU.WHXB201301182
16. [16]
  (16) Fang, Y. Y.; Hu, X. G.; Yu, L.; Li,W. B.; Lin, R. S. Acta Phys. -Chim. Sin. 2007, 23, 84. [方盈盈, 胡新根, 于丽, 李文兵, 林瑞森. 物理化学学报, 2007, 23, 84.] doi: 10.3866/PKU.WHXB20070117
17. [17]
  (17) Panos, M.; Sen, T. Z.; Ahunbay, M. G. Langmuir 2012, 28 (34), 12619. doi: 10.1021/la301546v
18. [18]
  (18) Schuler, L. D.; Daura, X.; Van Gunsteren,W. F. Journal of Computational Chemistry 2001, 22 (11), 1205.
19. [19]
  (19) Van der Spoel, D.; Lindahl, E.; Hess, B.; Groenhof, G.; Mark, A. E.; Berendsen, H. J. Journal of Computational Chemistry 2005, 26 (16), 1701.
20. [20]
  (20) Hess, B.; Kutzner, C.; Van der Spoel, D.; Lindahl, E. Journal of Chemical Theory and Computation 2008, 4 (3), 435. doi: 10.1021/ct700301q
21. [21]
  (21) Lindahl, E.; Hess, B.; Van der Spoel, D. Molecular Modeling Annual 2001, 7 (8), 306.
22. [22]
  (22) Essmann, U.; Perera, L.; Berkowitz, M. L.; Darden, T.; Lee, H.; Pedersen, L. G. The Journal of Chemical Physics 1995, 103 (19), 8577. doi: 10.1063/1.470117
23. [23]
  (23) Berendsen, H. J.; Postma, J. P. M.; van Gunsteren,W. F.; DiNola, A. R. H. J.; Haak, J. R. The Journal of Chemical Physics 1984, 81 (8), 3684. doi: 10.1063/1.448118
24. [24]
  (24) Hess, B.; Bekker, H.; Berendsen, H. J.; Fraaije, J. G. Journal of Computational Chemistry 1997, 18 (12), 1463.
25. [25]
  (25) Dismer, F.; Hubbuch, J. Journal of Chromatography A 2007, 1149 (2), 312. doi: 10.1016/j.chroma.2007.03.074
26. [26]
  (26) Hower, J. C.; He, Y.; Bernards, M. T.; Jiang, S. The Journal of Chemical Physics 2006, 125 (21), 214704. doi: 10.1063/1.2397681
27. [27]
  (27) Vanderah, D. J.; La, H.; Naff, J.; Silin, V.; Rubinson, K. A. Journal of the American Chemical Society 2004, 126 (42), 13639. doi: 10.1021/ja047744n
28. [28]
  (28) Shao, Q.; He, Y.; White, A. D.; Jiang, S. The Journal of Physical Chemistry B 2010, 114 (49), 16625. doi: 10.1021/jp107272n
29. [29]
  (29) He, Y.; Hower, J.; Chen, S.; Bernards, M. T.; Chang, Y.; Jiang, S. Langmuir 2008, 24 (18), 10358. doi: 10.1021/la8013046
30. [30]
  (30) He, Y.; Chang, Y.; Hower, J. C.; Zheng, J.; Chen, S.; Jiang, S. Physical Chemistry Chemical Physics 2008, 10 (36),5539. doi: 10.1039/b807129b
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