Citation: YANG Da-Wei, CHEN Chao, XIE Qing-Ji, YAO Shou-Zhuo. Comparison of Enzymatic Activities and Electroactivities of Adsorbed Glucose Oxidase on Several Nanomaterial-Modified Electrodes[J]. Acta Physico-Chimica Sinica, ;2013, 29(08): 1727-1734. doi: 10.3866/PKU.WHXB201306042 shu

Comparison of Enzymatic Activities and Electroactivities of Adsorbed Glucose Oxidase on Several Nanomaterial-Modified Electrodes

1.
College of Chemistry and Chemical Engineering, Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research, Ministry of Education, Changsha 410081, P. R. China

Received Date: 23 March 2013
Available Online: 4 June 2013
Fund Project: 国家自然科学基金(21175042, 21075036) (21175042, 21075036)湖南省教育厅(11A069) (11A069)

The quartz-crystal microbalance technique was used to monitor the adsorption of glucose oxidase ( x) on bare Au, Au-electrodeposited Au (Au_ed/Au), multiwalled carbon nanotube (MWCNT)- modified Au (MWCNTs/Au), and Au-electrodeposited MWCNT-modified Au (Au_ed/MWCNTs/Au) electrodes. The mass of x at saturated adsorption was obtained in each case. The amperometric responses of these enzyme electrodes were examined, and the mass-specific bioactivities of the immobilized (adsorbed) x (MSBA_i) were evaluated through anodic potentiostatic detection of enzymatically generated H₂O₂ in the presence of glucose. The direct electrochemistry of adsorbed x was studied by cyclic voltammetry, to obtain the electroactivity percentage of the immobilized (adsorbed) x (EAP_i) in each case. We found that the amounts of enzyme adsorbed and the amperometric responses of these enzyme electrodes follow the order MWCNTs/Au > Au_ed/MWCNTs/Au > Au_ed/Au > Au; MSBA_i follows the order of Au > Au_ed/MWCNTs/Au > Au_ed/Au > MWCNTs/Au; and EAP_i follows the order of MWCNTs/Au > Au_ed/MWCNTs/Au > Au_ed/Au > Au. The experimental data are interpreted based on hydrophobic/hydrophilic interactions among enzyme molecules and the nanomaterials, as well as the amount of adsorbed x. It was also verified that the total enzymatic activity of all the electrode-adsorbed enzyme molecules is positively related to the amperometric responses of the enzyme electrodes. This work is useful in studying the immobilization of enzyme on nanomaterials and thus-prepared amperometric enzyme electrodes.
- Quartz crystal microbalance,
- Glucose oxidase,
- Mass-specific bioactivity,
- Electroactivity percentage,
- Multiwalled carbon nanotubes,
- Electrodeposition of Au nanoparticles
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沈阳化工大学材料科学与工程学院沈阳 110142