Citation: LI Jin-Hua, LI JING, FANG Xuan, WANG Xiao-Hua, WEI Zhi-Peng. Fabrication and Characteristic of ZnO Glucose Oxidase Enzyme Electrode Based on Flexible Substrate[J]. Acta Physico-Chimica Sinica, ;2012, 28(06): 1393-1397. doi: 10.3866/PKU.WHXB201204062 shu

Fabrication and Characteristic of ZnO Glucose Oxidase Enzyme Electrode Based on Flexible Substrate

LI Jin-Hua ^1,2, ,
LI JING ¹ ,
FANG Xuan ^1,2 ,
WANG Xiao-Hua ¹ ,
WEI Zhi-Peng ¹

1.
1. School of Science, Changchun University of Science and Technology, Changchun 130022, P. R. China;
2. International Joint Research Center for Nanophotonics and Biophotonics, Changchun University of Science and Technology, Changchun 130022, P. R. China

Received Date: 9 March 2012
Available Online: 6 April 2012
Fund Project: 国家自然科学基金(61006065, 61076039, 10804071) (61006065, 61076039, 10804071) 高等学校博士学科点专项科研基金(20102216110001, 20112216120005) (20102216110001, 20112216120005) 吉林省科技发展计划(20091039, 20090555, 20101546, 20121816, 201201116) (20091039, 20090555, 20101546, 20121816, 201201116)吉林省教育厅项目(JYT2011-05, JYT2011-10, JYT2011-11)资助 (JYT2011-05, JYT2011-10, JYT2011-11)

Well-aligned ZnO nanorods (NRs) were grown on the ZnO seed layer of a polyimide (PI) coated flexible substrate using the hydrothermal method and used as a support matrix for the immobilization of glucose oxidase ( x). The crystal structures and surface morphologies of the so-formed ZnO nanorods were analyzed by X-ray diffraction (XRD) and scanning electron microscopy (SEM). x was immobilized on the surfaces of ZnO nanorods using electrostatic adsorption. Both the x and modified ZnO nanorods were characterized by UV-visible spectroscopy, and the absorption peaks of ZnO and x can be detected. Fourier transform infrared (FTIR) spectroscopy were used to characterize the chemical structure of the glucose oxide, which still maintained its biological activity. This study provides an experiment basis for the preparation of flexible glucose biosensors owing to the flexibility of the enzyme electrode, which showed a sensitive current response when tested by cyclic voltammetry.
- Glucose oxidase,
- Flexible substrate,
- Biosensor,
- ZnO
1. [1]
  (1) Yu, J.; Ju, H. Anal. Chem. 2002, 74, 3579. doi: 10.1021/ac011290k
2. [2]
  (2) Lee, H.; Yoon, S. W.; Kim, E. J.; Park, J. Nano Lett. 2007, 7, 778. doi: 10.1021/nl0630539
3. [3]
  (3) Vidur, K. L. Melliand-China 2005, 2,75
4. [4]
  (4) Rajesh, C.; Kaneto, K. Curr. Appl. Phys. 2005, 2, 178
5. [5]
  (5) Wang, J.; Fang, L.; Lopez, D. Analyst 1994, 119, 455
6. [6]
  (6) Consnier, S.; Innocent, C. Bioelectrochem. Bioenergy 1993, 3, 147
7. [7]
  (7) Kondo, T.; Horiuchi, S.; Yagi, I.; Ye, S.; Uosaki, K. J. Am. Chem. Soc 121 1999, 121, 391
8. [8]
  (8) Liu, Z. J.; Liu, B. H.; Kong, J. L.; Deng, J. Q. Anal. Chem. 2000, 72, 707
9. [9]
  (9) Wang, B. Q.; Li, B.; Wang, Z. X.; Xu, G. B.; Wang, Q.; Dong, S. J. Anal. Chem 1999, 71,1935
10. [10]
  (10) Muguruma, H.; Hiratsuka, A.; Karube, I. Anal. Chem 2000, 72, 2671
11. [11]
  (11) Yang, Q.; Atanasov, P.; Wilkins, E. Sens. Actuators B: Chem 1998, 46, 249
12. [12]
  (12) Jua, H. Q.; Liua, S. Q.; Geb, B. X.; Lisdatb, F.; Schellerb, F. W. Electroanalysis 2002, 14,141
13. [13]
  (13) Huang, M. H.; Mao, S.; Feick, H.; Yan, H.; Wu, Y.; Kind, H.; Weber, E.; Russo, R.; Yang, P. Science 2001, 292,1897. doi: 10.1126/science.1060367
14. [14]
  (14) Xu, C. X.; Sun, X. W. Appl. Phys. Lett. 2003, 83, 3806
15. [15]
  (15) Gao, P. X.; Ding, Y.; Mai, W.; Hughes, W. L.; Lao, C. S.; Wang, Z. L. Science 2005, 309, 1700
16. [16]
  (16) Sun, X. W.; Kwok, H. S. J. Appl. Phys 1999, 86, 408
17. [17]
  (17) Zhang, F.; Wang, X.; Ai, S.; Sun, Z.; Wan, Q.; Zhu, Z.; Xian, Y.; Jin, L.; Yamamoto, K. Anal. Chim. Acta 2004, 519, 155
18. [18]
  (18) Umar, A.; Rahman, M. M.; Kim, S. H.; Hahn, Y. B. J. Nanosci. Nanotechnol 2008, 8, 3216
19. [19]
  (19) Wei, A. Suna, X. W.; Wang, J. X.; Lei, Y.; Cai, X. P.; Li, C. M.; Dong, Z. L., Huang, W. Appl. Phys. Lett. 2006, 89, 123902
20. [20]
  (20) Liu, Z.; Liu, Y.; Yang, H.; Yang, Y.; Shen, G.; Yu, R. Electroanalysis 2005, 17, 1065
21. [21]
  (21) Cullity, B. D.; Stock, S. R. Elements of X-Ray Diffraction; Prentice Hall: New Jersey, 2001; p 619.
22. [22]
  (22) Topoglidis, E.; Cass, A. E. G.; O'Regan, B.; Durrant, J. R. J. Electroanal. Chem. 2001, 517, 20
23. [23]
  (23) Schvartzman, M.; Sidorov, V.; Ritter, D.; Paz, Y. J. Vac. Sci. Technol. 2001, 169, 68
24. [24]
  (24) Liu, T.; Liao, H.; Lin, C.; Hu, S.; Chen, S. Langmuir 2006, 22, 5804
25. [25]
  (25) Cao, X.; Wang, N.; Li, L.; Guo, L. Sensors and Actuators B 2008, 129, 268
26. [26]
  (26) Chi, Q.; Zhang, J.; Dong, S.; Wang, E. J. Chem. Soc. Faraday. Trans 1994, 90, 2057
27. [27]
  (27) Weber, J.; Jeedigunta, S.; Kumar, A. Journal of Nanomaterials 2008, 1
28. [28]
  (28) Dai, Z.; Liu, K.; Tang, Y.; Yang, X.; Bao, J.; Shen, J. J. Mater. Chem. 2008, 18, 1919
29. [29]
  (29) Kong, T.; Chen, Y.; Ye, Y.; Zhang, K.; Wang, H. Z.; Wang, X. Sens. Actuators B 2009, 38, 344
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