Citation:
LI Jin-Hua, LI JING, FANG Xuan, WANG Xiao-Hua, WEI Zhi-Peng. Fabrication and Characteristic of ZnO Glucose Oxidase Enzyme Electrode Based on Flexible Substrate[J]. Acta Physico-Chimica Sinica,
;2012, 28(06): 1393-1397.
doi:
10.3866/PKU.WHXB201204062
-
Well-aligned ZnO nanorods (NRs) were grown on the ZnO seed layer of a polyimide (PI) coated flexible substrate using the hydrothermal method and used as a support matrix for the immobilization of glucose oxidase ( x). The crystal structures and surface morphologies of the so-formed ZnO nanorods were analyzed by X-ray diffraction (XRD) and scanning electron microscopy (SEM). x was immobilized on the surfaces of ZnO nanorods using electrostatic adsorption. Both the x and modified ZnO nanorods were characterized by UV-visible spectroscopy, and the absorption peaks of ZnO and x can be detected. Fourier transform infrared (FTIR) spectroscopy were used to characterize the chemical structure of the glucose oxide, which still maintained its biological activity. This study provides an experiment basis for the preparation of flexible glucose biosensors owing to the flexibility of the enzyme electrode, which showed a sensitive current response when tested by cyclic voltammetry.
-
Keywords:
-
Glucose oxidase
, - Flexible substrate,
- Biosensor,
- ZnO
-
-
-
-
[1]
(1) Yu, J.; Ju, H. Anal. Chem. 2002, 74, 3579. doi: 10.1021/ac011290k
-
[2]
(2) Lee, H.; Yoon, S. W.; Kim, E. J.; Park, J. Nano Lett. 2007, 7, 778. doi: 10.1021/nl0630539
-
[3]
(3) Vidur, K. L. Melliand-China 2005, 2,75
-
[4]
(4) Rajesh, C.; Kaneto, K. Curr. Appl. Phys. 2005, 2, 178
-
[5]
(5) Wang, J.; Fang, L.; Lopez, D. Analyst 1994, 119, 455
-
[6]
(6) Consnier, S.; Innocent, C. Bioelectrochem. Bioenergy 1993, 3, 147
-
[7]
(7) Kondo, T.; Horiuchi, S.; Yagi, I.; Ye, S.; Uosaki, K. J. Am. Chem. Soc 121 1999, 121, 391
-
[8]
(8) Liu, Z. J.; Liu, B. H.; Kong, J. L.; Deng, J. Q. Anal. Chem. 2000, 72, 707
-
[9]
(9) Wang, B. Q.; Li, B.; Wang, Z. X.; Xu, G. B.; Wang, Q.; Dong, S. J. Anal. Chem 1999, 71,1935
-
[10]
(10) Muguruma, H.; Hiratsuka, A.; Karube, I. Anal. Chem 2000, 72, 2671
-
[11]
(11) Yang, Q.; Atanasov, P.; Wilkins, E. Sens. Actuators B: Chem 1998, 46, 249
-
[12]
(12) Jua, H. Q.; Liua, S. Q.; Geb, B. X.; Lisdatb, F.; Schellerb, F. W. Electroanalysis 2002, 14,141
-
[13]
(13) Huang, M. H.; Mao, S.; Feick, H.; Yan, H.; Wu, Y.; Kind, H.; Weber, E.; Russo, R.; Yang, P. Science 2001, 292,1897. doi: 10.1126/science.1060367
-
[14]
(14) Xu, C. X.; Sun, X. W. Appl. Phys. Lett. 2003, 83, 3806
-
[15]
(15) Gao, P. X.; Ding, Y.; Mai, W.; Hughes, W. L.; Lao, C. S.; Wang, Z. L. Science 2005, 309, 1700
-
[16]
(16) Sun, X. W.; Kwok, H. S. J. Appl. Phys 1999, 86, 408
-
[17]
(17) Zhang, F.; Wang, X.; Ai, S.; Sun, Z.; Wan, Q.; Zhu, Z.; Xian, Y.; Jin, L.; Yamamoto, K. Anal. Chim. Acta 2004, 519, 155
-
[18]
(18) Umar, A.; Rahman, M. M.; Kim, S. H.; Hahn, Y. B. J. Nanosci. Nanotechnol 2008, 8, 3216
-
[19]
(19) Wei, A. Suna, X. W.; Wang, J. X.; Lei, Y.; Cai, X. P.; Li, C. M.; Dong, Z. L., Huang, W. Appl. Phys. Lett. 2006, 89, 123902
-
[20]
(20) Liu, Z.; Liu, Y.; Yang, H.; Yang, Y.; Shen, G.; Yu, R. Electroanalysis 2005, 17, 1065
-
[21]
(21) Cullity, B. D.; Stock, S. R. Elements of X-Ray Diffraction; Prentice Hall: New Jersey, 2001; p 619.
-
[22]
(22) Topoglidis, E.; Cass, A. E. G.; O'Regan, B.; Durrant, J. R. J. Electroanal. Chem. 2001, 517, 20
-
[23]
(23) Schvartzman, M.; Sidorov, V.; Ritter, D.; Paz, Y. J. Vac. Sci. Technol. 2001, 169, 68
-
[24]
(24) Liu, T.; Liao, H.; Lin, C.; Hu, S.; Chen, S. Langmuir 2006, 22, 5804
-
[25]
(25) Cao, X.; Wang, N.; Li, L.; Guo, L. Sensors and Actuators B 2008, 129, 268
-
[26]
(26) Chi, Q.; Zhang, J.; Dong, S.; Wang, E. J. Chem. Soc. Faraday. Trans 1994, 90, 2057
-
[27]
(27) Weber, J.; Jeedigunta, S.; Kumar, A. Journal of Nanomaterials 2008, 1
-
[28]
(28) Dai, Z.; Liu, K.; Tang, Y.; Yang, X.; Bao, J.; Shen, J. J. Mater. Chem. 2008, 18, 1919
-
[29]
(29) Kong, T.; Chen, Y.; Ye, Y.; Zhang, K.; Wang, H. Z.; Wang, X. Sens. Actuators B 2009, 38, 344
-
[1]
-
-
-
[1]
Meiqing Yang , Lu Wang , Haozi Lu , Yaocheng Yang , Song Liu . Recent Advances of Functional Nanomaterials for Screen-Printed Photoelectrochemical Biosensors. Acta Physico-Chimica Sinica, 2025, 41(2): 100018-. doi: 10.3866/PKU.WHXB202310046
-
[2]
Zijian Jiang , Yuang Liu , Yijian Zong , Yong Fan , Wanchun Zhu , Yupeng Guo . Preparation of Nano Zinc Oxide by Microemulsion Method and Study on Its Photocatalytic Activity. University Chemistry, 2024, 39(5): 266-273. doi: 10.3866/PKU.DXHX202311101
-
[3]
Qiaoqiao BAI , Anqi ZHOU , Xiaowei LI , Tang LIU , Song LIU . Construction of pressure-temperature dual-functional flexible sensors and applications in biomedicine. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2259-2274. doi: 10.11862/CJIC.20240128
-
[4]
Yu Wang , Shoulei Zhang , Tianming Lv , Yan Su , Xianyu Liu , Fuping Tian , Changgong Meng . Introduce a Comprehensive Inorganic Synthesis Experiment: Synthesis of Nano Zinc Oxide via Microemulsion Using Waste Soybean Oil. University Chemistry, 2024, 39(7): 316-321. doi: 10.3866/PKU.DXHX202311035
-
[5]
Tengjiao Wang , Tian Cheng , Rongjun Liu , Zeyi Wang , Yuxuan Qiao , An Wang , Peng Li . Conductive Hydrogel-based Flexible Electronic System: Innovative Experimental Design in Flexible Electronics. University Chemistry, 2024, 39(4): 286-295. doi: 10.3866/PKU.DXHX202309094
-
[6]
Ping ZHANG , Chenchen ZHAO , Xiaoyun CUI , Bing XIE , Yihan LIU , Haiyu LIN , Jiale ZHANG , Yu'nan CHEN . Preparation and adsorption-photocatalytic performance of ZnAl@layered double oxides. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1965-1974. doi: 10.11862/CJIC.20240014
-
[7]
Xiaoning TANG , Shu XIA , Jie LEI , Xingfu YANG , Qiuyang LUO , Junnan LIU , An XUE . Fluorine-doped MnO2 with oxygen vacancy for stabilizing Zn-ion batteries. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1671-1678. doi: 10.11862/CJIC.20240149
-
[8]
Xingchao Zhao , Xiaoming Li , Ming Liu , Zijin Zhao , Kaixuan Yang , Pengtian Liu , Haolan Zhang , Jintai Li , Xiaoling Ma , Qi Yao , Yanming Sun , Fujun Zhang . 倍增型全聚合物光电探测器及其在光电容积描记传感器上的应用. Acta Physico-Chimica Sinica, 2025, 41(1): 2311021-. doi: 10.3866/PKU.WHXB202311021
-
[9]
Jiarong Feng , Yejie Duan , Chu Chu , Dezhen Xie , Qiu'e Cao , Peng Liu . Preparation and Application of a Streptomycin Molecularly Imprinted Electrochemical Sensor: A Suggested Comprehensive Analytical Chemical Experiment. University Chemistry, 2024, 39(8): 295-305. doi: 10.3866/PKU.DXHX202401016
-
[10]
Zhuoya WANG , Le HE , Zhiquan LIN , Yingxi WANG , Ling LI . Multifunctional nanozyme Prussian blue modified copper peroxide: Synthesis and photothermal enhanced catalytic therapy of self-provided hydrogen peroxide. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2445-2454. doi: 10.11862/CJIC.20240194
-
[11]
Chunmei GUO , Weihan YIN , Jingyi SHI , Jianhang ZHAO , Ying CHEN , Quli FAN . Facile construction and peroxidase-like activity of single-atom platinum nanozyme. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1633-1639. doi: 10.11862/CJIC.20240162
-
[12]
Zhongxin YU , Wei SONG , Yang LIU , Yuxue DING , Fanhao MENG , Shuju WANG , Lixin YOU . Fluorescence sensing on chlortetracycline of a Zn-coordination polymer based on mixed ligands. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2415-2421. doi: 10.11862/CJIC.20240304
-
[13]
Wei HE , Jing XI , Tianpei HE , Na CHEN , Quan YUAN . Application of solar-driven inorganic semiconductor-microbe hybrids in carbon dioxide fixation and biomanufacturing. Chinese Journal of Inorganic Chemistry, 2025, 41(1): 35-44. doi: 10.11862/CJIC.20240364
-
[14]
Xiaowei TANG , Shiquan XIAO , Jingwen SUN , Yu ZHU , Xiaoting CHEN , Haiyan ZHANG . A zinc complex for the detection of anthrax biomarker. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1850-1860. doi: 10.11862/CJIC.20240173
-
[15]
Lu XU , Chengyu ZHANG , Wenjuan JI , Haiying YANG , Yunlong FU . Zinc metal-organic framework with high-density free carboxyl oxygen functionalized pore walls for targeted electrochemical sensing of paracetamol. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 907-918. doi: 10.11862/CJIC.20230431
-
[16]
Minna Ma , Yujin Ouyang , Yuan Wu , Mingwei Yuan , Lijuan Yang . Green Synthesis of Medical Chemiluminescence Reagents by Photocatalytic Oxidation. University Chemistry, 2024, 39(5): 134-143. doi: 10.3866/PKU.DXHX202310093
-
[17]
Yunting Shang , Yue Dai , Jianxin Zhang , Nan Zhu , Yan Su . Something about RGO (Reduced Graphene Oxide). University Chemistry, 2024, 39(9): 273-278. doi: 10.3866/PKU.DXHX202306050
-
[18]
Linbao Zhang , Weisi Guo , Shuwen Wang , Ran Song , Ming Li . Electrochemical Oxidation of Sulfides to Sulfoxides. University Chemistry, 2024, 39(11): 204-209. doi: 10.3866/PKU.DXHX202401009
-
[19]
Chuanming GUO , Kaiyang ZHANG , Yun WU , Rui YAO , Qiang ZHAO , Jinping LI , Guang LIU . Performance of MnO2-0.39IrOx composite oxides for water oxidation reaction in acidic media. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1135-1142. doi: 10.11862/CJIC.20230459
-
[20]
Zhihuan XU , Qing KANG , Yuzhen LONG , Qian YUAN , Cidong LIU , Xin LI , Genghuai TANG , Yuqing LIAO . Effect of graphene oxide concentration on the electrochemical properties of reduced graphene oxide/ZnS. Chinese Journal of Inorganic Chemistry, 2024, 40(7): 1329-1336. doi: 10.11862/CJIC.20230447
-
[1]
Metrics
- PDF Downloads(816)
- Abstract views(2379)
- HTML views(56)