Citation: ZENG Chi, ZHU Jian-Yu. Microcalorimetric Study of the Influence of Extracellular NaCl Concentration on the Growth Metabolism of Halobacterium halobiumZENG Chi1,* ZHU Jian-Yu[J]. Acta Physico-Chimica Sinica, ;2011, 27(06): 1525-1530. doi: 10.3866/PKU.WHXB20110618 shu

Microcalorimetric Study of the Influence of Extracellular NaCl Concentration on the Growth Metabolism of Halobacterium halobiumZENG Chi1,* ZHU Jian-Yu

ZENG Chi ^1, ,
ZHU Jian-Yu ^2,

1.
1. School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, P. R. China;
2. School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, P. R. China

Received Date: 16 February 2011
Available Online: 29 April 2011
Fund Project: 国家自然科学基金(31000050, 30700008) (31000050, 30700008)湖北省教育厅科学技术研究项目(B20111701)资助 (B20111701)

Microcalorimetry was used to study the influence of extracellular NaCl concentration on the growth metabolism of Halobacterium halobium (Hbt. halobium) using a TAM air isothermal microcalorimeter. The metabolic thermogenic curves of Hbt. halobium growth in different concentrations of NaCl were obtained using the ampoule method. The thermokinetic equations and parameters of Hbt. halobium growth were calculated and the relationship between each thermokinetic parameter and the concentration of NaCl was obtained. The results showed that the optimum NaCl concentration for Hbt. halobium growth was not a wide range from 3.5 mol L^-1 to about 5.2 mol·L^-1 (NaCl saturation), as is generally acknowledged, but just around 3.9 mol·L^-1. For an extracellular NaCl concentration above 3.9 mol·L^-1, the growth metabolism of Hbt. halobium decreased constantly with an increase in the NaCl concentration. Further investigation by transmission electron microscopy revealed that the Hbt. halobium cells growing in approaching NaCl saturation underwent plasmolysis, which interpreted the finding of microcalorimetry perfectly. All these results led to a new interpretation of the structural transformations of Hbt. halobium upon NaCl concentration altering.
- Microcalorimetry,
- Haloarchaea,
- Extracellular NaCl concentration,
- Cell surface structure
1. [1]
  
  (1) Oren, A. The Order Halobacteriales. In The Prokaryotes: A Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Applications, 3rd ed.; Dworkin, M., Falkow, S., Rosenberg, E., Schleifer, K. H., Stackebrandt, E. Eds.; Springer-Verlag: New York, 1999.
2. [2]
  (2) Grant,W. D.; Kamekura, M.; McGenity, T. J.; Ventosa, A. Halobacteria. In Bergey’ s Manual of Systematic Bacteriology, 2nd ed.; Garrity, G. M. Eds.; Springer-Verlag: New York, 2001.
3. [3]
  (3) Stoeckenius,W.; Rowen, R. J. Cell Biol. 1967, 34, 365.
4. [4]
  (4) Grant,W. D. Phil. Trans. R. Soc. Lond. B 2004, 359, 1249.
5. [5]
  (5) Willey, J.; Sherwood, L.;Woolverton, C. Prescott′s Microbiology, 8th ed.; McGraw-Hill: New York, 2011; chap. 6.
6. [6]
  (6) Zeng, C.; Zhu, J. C.; Liu, Y.; Yang, Y.; Zhu, J. Y.; Huang, Y. P.; Shen, P. J. Therm. Anal. Cal. 2006, 84, 625.
7. [7]
  (7) Wads?, I. Thermochim. Acta 2002, 394, 305.
8. [8]
  (8) Braissant, O.;Wirz, D.; G?pfert, B.; Daniels, A. U. FEMS Microbiol. Lett. 2010, 303, 1.
9. [9]
  (9) Rodriguez-Valera, F.; Ruiz-Berraquero, F.; Ramos-Cormenzana, A. J. Gen. Microbiol. 1980, 119, 535.
10. [10]
  (10) Gibbons, N. E.; Payne, J. I. Can. J. Microbiol. 1961, 7, 483.
11. [11]
  (11) Pritchard, J. Tur r Pressure. In Encyclopedia of Life Sciences; Nature Publishing Group: London, 2001.
12. [12]
  (12) Koch, A. L. Growth Measurement. In Manual of Methods for General Bacteriology; Gerhardt, P. Eds.; American Society for Microbiology:Washington D.C., 1981; chap. 11.
13. [13]
  (13) Shand, R. F.; Perez, A. M. Haloarchaeal Growth Physiology. In Enigmatic Organisms and Life in Extreme Environments; Seckback, J. Eds.; Kluwer Academic Publishers: Dordrecht, 1999; pp 413-424.
14. [14]
  (14) Dyall-Smith, M. The Halohandbook: Protocols for Haloarchaeal Genetics, version 7.1; 2009; pp 109-111. http://www.haloarchaea.com/resources/halohandbook
15. [15]
  (15) Huang, Y. P.; Liu, Y.; Shen, P.; Qu, S. S. J. Therm. Anal. Cal. 2003, 74, 163.
16. [16]
  (16) Liu, P.; Liu, Y.; Xie, Z. X.; Chen, Y. G.; Zhao, R. M.; Shen, P.; Qu, S. S. Chin. J. Chem. 2003, 21, 693.
17. [17]
  [刘鹏, 刘义, 谢之雄, 陈酉贵, 赵儒铭, 沈萍, 屈松生. 中国化学, 2003, 21, 693.]
18. [18]
  (17) Liu, Y.; Yu, H. G.; Huang, Y. P.; Shen, P.; Qu, S. S. Biol. Trace Elem. Res. 2003, 92, 83.
1. [1]
  Xinlong WANG , Zhenguo CHENG , Guo WANG , Xiaokuen ZHANG , Yong XIANG , Xinquan WANG . Enhancement of the fragile interface of high voltage LiCoO₂ by surface gradient permeation of trace amounts of Mg/F. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 571-580. doi: 10.11862/CJIC.20230259
2. [2]
  Xinting XIONG , Zhiqiang XIONG , Panlei XIAO , Xuliang NIE , Xiuying SONG , Xiuguang YI . Synthesis, crystal structures, Hirshfeld surface analysis, and antifungal activity of two complexes Na(Ⅰ)/Cd(Ⅱ) assembled by 5-bromo-2-hydroxybenzoic acid ligands. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1661-1670. doi: 10.11862/CJIC.20240145
3. [3]
  Ruilin Han , Xiaoqi Yan . Comparison of Multiple Function Methods for Fitting Surface Tension and Concentration Curves. University Chemistry, 2024, 39(7): 381-385. doi: 10.3866/PKU.DXHX202311023
4. [4]
  Xinyu ZENG , Guhua TANG , Jianming OUYANG . Inhibitory effect of Desmodium styracifolium polysaccharides with different content of carboxyl groups on the growth, aggregation and cell adhesion of calcium oxalate crystals. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1563-1576. doi: 10.11862/CJIC.20230374
5. [5]
  Kun Li , Na Gao , Shuangyan Huan , Yuzhi Wang . Design of Ideological and Political Education for the Experiment of Detecting Cadmium with Anodic Stripping Voltammetry. University Chemistry, 2024, 39(2): 155-161. doi: 10.3866/PKU.DXHX202307068
6. [6]
  Liang MA , Honghua ZHANG , Weilu ZHENG , Aoqi YOU , Zhiyong OUYANG , Junjiang CAO . Construction of highly ordered ZIF-8/Au nanocomposite structure arrays and application of surface-enhanced Raman spectroscopy. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1743-1754. doi: 10.11862/CJIC.20240075
7. [7]
  Zhuo WANG , Junshan ZHANG , Shaoyan YANG , Lingyan ZHOU , Yedi LI , Yuanpei LAN . Preparation and photocatalytic performance of CeO₂-reduced graphene oxide by thermal decomposition. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1708-1718. doi: 10.11862/CJIC.20240067
8. [8]
  Yang Lv , Yingping Jia , Yanhua Li , Hexiang Zhong , Xinping Wang . Integrating the Ideological Elements with the “Chemical Reaction Heat” Teaching. University Chemistry, 2024, 39(11): 44-51. doi: 10.12461/PKU.DXHX202402059
9. [9]
  Limei CHEN , Mengfei ZHAO , Lin CHEN , Ding LI , Wei LI , Weiye HAN , Hongbin WANG . Preparation and performance of paraffin/alkali modified diatomite/expanded graphite composite phase change thermal storage material. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 533-543. doi: 10.11862/CJIC.20230312
10. [10]
  Liyang ZHANG , Dongdong YANG , Ning LI , Yuanyu YANG , Qi MA . Crystal structures, luminescent properties and Hirshfeld surface analyses of three cadmium(Ⅱ) complexes based on 2-(3-(pyridin-2-yl)-1H-pyrazol-1-yl)benzoate. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1943-1952. doi: 10.11862/CJIC.20240079
11. [11]
  Yaofeng Yuan , Keyin Ye , Chunfa Xu , Hong Yan , Yuanming Li . Fostering an International Perspective in Postgraduate Student Teaching: A Case Study of the Organic Structure Analysis Course. University Chemistry, 2024, 39(6): 145-150. doi: 10.3866/PKU.DXHX202402024
12. [12]
  Yuping Wei , Yiting Wang , Jialiang Jiang , Jinxuan Deng , Hong Zhang , Xiaofei Ma , Junjie Li . Interdisciplinary Teaching Practice——Flexible Wearable Electronic Skin for Low-Temperature Environments. University Chemistry, 2024, 39(10): 261-270. doi: 10.12461/PKU.DXHX202404007
13. [13]
  Shuyu Liu , Xiaomin Sun , Bohan Song , Gaofeng Zeng , Bingbing Du , Chongshen Guo , Cong Wang , Lei Wang . Design and Fabrication of Phospholipid-Vesicle-based Artificial Cells towards Biomedical Applications. University Chemistry, 2024, 39(11): 182-188. doi: 10.12461/PKU.DXHX202404113
14. [14]
  Nana Wang , Gaosheng Zhang , Huosheng Li , Tangfu Xiao . Discussion on the Teaching Reform of Environmental Functional Materials within the Context of “Double First-Class” Initiative: Emphasizing the Integration of Industry, Academia, Research, and Application. University Chemistry, 2024, 39(6): 137-144. doi: 10.3866/PKU.DXHX202312010
15. [15]
  Cunling Ye , Xitong Zhao , Hongfang Wang , Zhike Wang . A Formula for the Calculation of Complex Concentrations Arising from Side Reactions and Its Applications. University Chemistry, 2024, 39(4): 382-386. doi: 10.3866/PKU.DXHX202310043
16. [16]
  Siyi ZHONG , Xiaowen LIN , Jiaxin LIU , Ruyi WANG , Tao LIANG , Zhengfeng DENG , Ao ZHONG , Cuiping HAN . Targeting imaging and detection of ovarian cancer cells based on fluorescent magnetic carbon dots. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1483-1490. doi: 10.11862/CJIC.20240093
17. [17]
  Shipeng WANG , Shangyu XIE , Luxian LIANG , Xuehong WANG , Jie WEI , Deqiang WANG . Piezoelectric effect of Mn, Bi co-doped sodium niobate for promoting cell proliferation and bacteriostasis. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1919-1931. doi: 10.11862/CJIC.20240094
18. [18]
  Peng GENG , Guangcan XIANG , Wen ZHANG , Haichuang LAN , Shuzhang XIAO . Hollow copper sulfide loaded protoporphyrin for photothermal-sonodynamic therapy of cancer cells. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1903-1910. doi: 10.11862/CJIC.20240155
19. [19]
  Zhihuan XU , Qing KANG , Yuzhen LONG , Qian YUAN , Cidong LIU , Xin LI , Genghuai TANG , Yuqing LIAO . Effect of graphene oxide concentration on the electrochemical properties of reduced graphene oxide/ZnS. Chinese Journal of Inorganic Chemistry, 2024, 40(7): 1329-1336. doi: 10.11862/CJIC.20230447
20. [20]
  Di WU , Ruimeng SHI , Zhaoyang WANG , Yuehua SHI , Fan YANG , Leyong ZENG . Construction of pH/photothermal dual-responsive delivery nanosystem for combination therapy of drug-resistant bladder cancer cell. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1679-1688. doi: 10.11862/CJIC.20240135

Get Citation

PDF

XML

Metrics

PDF Downloads(860)
Abstract views(2165)
HTML views(29)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142