Citation: ZENG Han, LIAO Ling-Wen, LI Ming-Fang, TAO Qian, KANG Jing, CHEN Yan-Xia. Poly Aryl Amide and Multiwalled Carbon Nanotube Composite Supported Laccase Electrode and Its Electrochemical Behavior[J]. Acta Physico-Chimica Sinica, ;2010, 26(12): 3217-3224. doi: 10.3866/PKU.WHXB20101208 shu

Poly Aryl Amide and Multiwalled Carbon Nanotube Composite Supported Laccase Electrode and Its Electrochemical Behavior

1.
Hefei National Laboratory for Physical Sciences at Microscale, Department of Chemical Physics, University of Science and Technology of China, Hefei 230026, P. R. China

Received Date: 7 July 2010
Available Online: 29 October 2010
Fund Project: 国家自然科学基金(20773116) (20773116)国家杰出青年基金(20474060)资助项目 (20474060)

A novel strategy for the immobilization of laccase onto a glassy carbon electrode with high stability and electrocatalytic performance is presented. Laccase is attached to a matrix of mixed poly aryl amide (PAA) and multiwalled carbon nanotubes (MWCNTs) (denoted Lac/PAA-MWCNTs/GCE) by covalently bonding the surface amine group of laccase to the terminal carboxyl group of PAA and hydrophobic-hydrophobic interaction between MWCNTs and the laccase. The PAA backbone avoids the detachment and denaturing of the laccase, and the intermixed MWCNTs provide high electronic conductivity. The loading of laccase is 56.0 mg·g^-1 and more than 68% shows electrochemical activity. The electrode delivers direct electron transfer between the redox center of the laccase and the electrode with two pairs of redox peaks at 0.73 and 0.38 V, which is close to the formal potential of the T₁ and T₂ Cu-sites (0.78 and 0.39 V (vs NHE)), respectively. The onset potential for O₂ reduction reaction (ORR) is ca 0.55 V in a phosphate buffer solution (pH=4.4). The Michaelis constant (K_M) of the Lac/PAA-MWCNTs/GEs for O₂ is 55.8 μmol·L^-1 and the detection limit of oxygen reaches 0.57 μmol·L^-1. After 2 months of storage at 4 °C the ORR activity of the Lac/PAA-MWCNTs/GC electrode retains ca 86% of its initial values and the peak potential of the ORR shifts negatively by ca 50 mV. Given the excellent catalytic performance towards ORR and its high stability this strategy will be widely applicable to the development of an enzyme-based cathode for biofuel cells and amperometric biosensors for oxygen.
- Laccase,
- Direct electron transfer,
- Oxygen reduction reaction,
- Bioelectrocatalysis,
- Biosensor
1. [1]
  
  1. Xu, F. Biochemistry, 1996, 35: 7608
2. [2]
  2. Piontek, K.; Antorini, M.; Choinowski, T. J. Biol. Chem., 2002, 277: 37663
3. [3]
  3. Zheng,W.; Li, Q. F.; Yan, Y. M.; Zhang, J.; Mao, L. Q. Electroanalysis, 2006, 18: 587
4. [4]
  4. Yarapolov, A. I.; Kharybin, A. N.; Emneus, J.; Marko-Varga, G.; rton, L. Bioelectrochem. Bioenerg., 1996, 40: 49
5. [5]
  5. Shleev, S.; Kasmi, A. E.; Ruzgas, T.; rton, L. Electrochem. Commun., 2004, 6: 934
6. [6]
  6. Liu, Y.; Qu, X. H.; Guo, H.W.; Chen, H. J.; Liu, B. F.; Dong, S. J. Biosensors & Bioelectronics, 2006, 21: 2195
7. [7]
  7. Rahman, M. A.; Noh, H. B.; Shim, Y. B. Anal. Chem., 2008, 80: 8020
8. [8]
  8. Tarasevich, M. R.; Bogdanovskaya, V. A.; Kuznetsova, L. N. Russ. J. Phys. Chem., 2001, 37: 969
9. [9]
  9. Stolarczyk, K.; Nazaruk, E.; Rogalski, J.; Bileswicz, R. Electrochimica Acta, 2008, 53: 3983
10. [10]
  10. Blanford, C. F.; Heath, R. S.; Armstrong, F. A. Chem. Commun., 2007: 1710
11. [11]
  11. Blanford, C. F.; Foster, C. E.; Heath, R. E.; Armstrong, F. A. Faraday Discuss., 2008, 140: 319
12. [12]
  12. Farneth,W. E.; Diner, B. A.; Gierke, T. D.; D'Amore, M. B. J. Electroanal. Chem., 2005, 581: 190
13. [13]
  13. Katz, E.; Sheeney-Haj-Ichia, L.;Willner, I. Angew. Chem. Int. Edit., 2004, 43: 3292
14. [14]
  14. Barriere, F.; Ferry, Y.; Rochefort, D.; Leech, D. Electrochem. Commun., 2004, 6: 237
15. [15]
  15. Ohara, T. J.; Raja palan, R.; Heller, A. Anal. Chem., 1993, 65: 3512
16. [16]
  16. Trudeau, F.; Daigle, F.; Leech, D. Anal. Chem., 1997, 69: 882
17. [17]
  17. Ackermann, Y.; Guschin, D. A.; Eckhard, K.; Shleev, S.; Schuhmann, W. Electrochem. Commun., 2010, 12: 640
18. [18]
  18. Klis, M.; Karbarz, M.; Stojek, Z.; Rogalski, J.; Bilewicz, R. J. Phys. Chem. B, 2009, 113: 6062
19. [19]
  19. Qiu, H. J.; Xu , C. X.; Huang, X. R.; Ding, Y.; Qu Y. B.; Gao, P. J. J. Phys. Chem. C, 2008, 112: 14781
20. [20]
  20. Stolarczyk, K.; Nazaruk, E.; Rogalski, J.; Bilewicz, R. Electrochem. Commun., 2007, 9: 115
21. [21]
  21. Fei, J. F.; Song, H. Y.; Palmore, G. T. R. Chem. Mater., 2007, 19(7): 1565
22. [22]
  22. Katz, E.;Willner, I.; Kotlyar, A. B. J. Electroanal. Chem., 1999, 479: 64
23. [23]
  23. Karnicka, K.; Miecznikowski, K.; Kowalewska, B.; Skunik, M.; Opallo, M.; Rogalski, J.; Schuhmann,W.; Kulesza, P. J. Anal. Chem., 2008, 80: 7643
24. [24]
  24. Liu, Y.;Wang, M. K.; Zhao, F.; Xu, Z. A.; Dong, S. J. Biosensors & Bioelectronics, 2005, 21: 984
25. [25]
  25. Qiu, H. J.; Xu , C. X.; Huang, X. R.; Ding, Y.; Gao, P. J. J. Phys. Chem. C, 2009, 113: 2521
26. [26]
  26. Liu, J.; Rinzler, A. G.; Dai, H. J.; Hafner, J. H.; Bradley, R. K.; Boul, P. J.; Lu, A.; Iverson, T.; Shelimov, K.; Huffman, C. B.; Rodriguez-Macias, F.; Shon, Y. S.; Lee, T. R.; Colbert, D. T.; Smalley, R. E. Science, 1998, 280: 1253
27. [27]
  27. Huang, J.; Zhou, J. Y.; Xiao, H. Y.; Long, S. Y.;Wang, J. T. Acta Chimica Sinica, 2005, 63(14): 1343. [黄俊, 周菊英, 肖海燕, 龙胜亚, 王军涛. 化学学报, 2005, 63(14): 1343]
28. [28]
  28. Ivanov, I.; Vidakovic-Koch, T.; Sundmacher, K. Energies, 2010, 3: 803
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沈阳化工大学材料科学与工程学院沈阳 110142