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Citation: HAN Yu-Mei, XU Xiu-Ying, ZHANG Shao-Feng, YAN Dan, XIAO Xiao-He, PENG Cheng. Microcalorimetric Investigation of Effect of Toad Bufanolide Diene on Tetrahymena Thermophila BF5 Growth[J]. Acta Physico-Chimica Sinica, ;2010, 26(06): 1693-1698. doi: 10.3866/PKU.WHXB20100634 shu

Microcalorimetric Investigation of Effect of Toad Bufanolide Diene on Tetrahymena Thermophila BF5 Growth

1.
College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, P. R. China; PLA Institute of Chinese Materia Medica, 302 Hospital of PLA, Beijing 100039, P. R. China; College of Chemistry and Biotechnology, Chongqing University of Technology, Chongqing 400050, P. R. China

Received Date: 8 February 2010
Available Online: 6 May 2010

The effects of various concentrations of toad bufanolide diene (BD) on the growth of Tetrahymena thermophila BF5 were investigated by means of a thermal activity monitor (TAM) system. The extent and duration of toxic effects on metabolismwere evaluated by studying the growth rate constant (k), maximumheat-output power (Pmax) and half inhibitory concentration (IC50). Experimental results showed that the values of k and Pmax decreased as the concentrations of BDincreased. Furthermore, the growth rate constant (k) was linked to the concentration of BD: rcinobufagin=0.9910 (IC50=119.2 μg·mL-1); rbufalin=0.9923 (IC50=106.14 μg·mL-1); rbufotalin=0.9977 (IC50=73.80 μg·mL-1); rgamabufotalin=0.9923 (IC50=54.75 μg·mL-1). The sequence of toxicity for the four BDs was: gamabufotalin>bufotalin>bufalin>cinobufagin. The α-hydroxy group at C11, β-hydroxy group at C14, β-acetoxy at C16 and the non-dehydrolysis between C14 and C15 can possibly improve the toxicity of BD towards Tetrahymena thermophila BF5.
- Microcalorimetry,
- Bufanolide diene,
- Tetrahymena thermophila BF5,
- Growth
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