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Citation: CAO Tuan-Wu, YANG Ji-Dong. Interaction between Malachite Green and Bovine Serum Albumin[J]. Acta Physico-Chimica Sinica, ;2008, 24(04): 715-719. doi: 10.3866/PKU.WHXB20080430 shu

Interaction between Malachite Green and Bovine Serum Albumin

1.
Department of Chemistry and Environment, Yangtze Normal University, Chongqing 408100, P. R. China

Received Date: 28 August 2007
Available Online: 19 February 2008

The interaction between malachite green (MG) and bovine serum albumin (BSA) was investigated via fluorescence and ultraviolet-visible absorption spectra in buffer solutions at different temperatures. The experimental results showed that static quenching was involved in addingMGin BSAsolution. The binding constants (KA) were 7.69×104 L·mol-1 (10 ℃), 5.31×10⁴ L·mol-1 (20 ℃), and 4.85×10⁴ L·mol-1 (37 ℃), respectively, and they reacted at a molar ratio of 1:1. The energy transfer efficiency E was 0.1635 and the binding distance r was 2.30 nm between MG and BSA according to Forster non-radiative energy transfer mechanism. The interaction between MG and BSA was driven mainly by hydrogen bonds and Vander Waals force according to thermodynamic parameters.
- Malachite green; Bovine serumalbumin; Fluorescence quenching; Interaction
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