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Citation: LIU Jing, XU Gui-ying, LIU Jun, YU Li, ZHANG Hong-lin, YU Xiu-fang. Interaction between Tween-20 and BSA[J]. Acta Physico-Chimica Sinica, ;2005, 21(08): 862-866. doi: 10.3866/PKU.WHXB20050808 shu

Interaction between Tween-20 and BSA

1.
Key Laboratory of Colloid and Interface Chemistry Ministry of Education, Shandong University, Jinan 250100; Department of Chemistry, Qufu Normanl University, Qufu 273165; Pharmic Institute, Hebei Medical University, Shijiazhuang 050017

Received Date: 24 December 2004
Available Online: 15 August 2005

The interaction between Tween-20 and BSA was studied by surface tension and isothermal titration microcalorimetry. The results show that there is a weak interaction between Tween-20 and BSA and the Tween-20 molecules can replace BSA molecules adsorbed on the surface. A competition adsorption between Tween-20 and BSA occurred at the surface of mixed solution. The tendency of change of the properties of the BSA/Tween-20 mixed system with temperature is similar to that of the single non-ionic surfactant. In the range of BSA concentration from 3.7×10^-5 kg•L^-1 to 1.1×10^-5 kg•L^-1, the cmc of Tween-20 does not change with BSA concentrations. When the amount of Tween-20 is fixed, the thermal effects of the interaction between BSA and Tween-20 does not depend on the BSA concentration. The thermodynamic parameters of the mixed solutions in the presence or absence of BSA indicate that the cmc of Tween-20 decreases and the value of entropy change of the system increases in the presence of BSA in solution.
- Tween-20;BSA;Surface tension;Isotherm titration microcalorimetry; Interaction
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