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Citation: Miao Wei, Yao Song-Nian. Study of β-1，4-glucosidase in Water/PC/Heptane-Butanol Reverse Micelles[J]. Acta Physico-Chimica Sinica, ;1999, 15(10): 930-937. doi: 10.3866/PKU.WHXB19991013 shu

Study of β-1，4-glucosidase in Water/PC/Heptane-Butanol Reverse Micelles

Miao Wei ,
Yao Song-Nian

1.
Center of Analysis and Measurement Wuhan University,Wuhan 430072

Received Date: 22 October 1998
Available Online: 15 October 1999

The maximum solubilization of water, acetic buffer and salicin in H 2O/PC/Heptan Butanol reverse micelles were measured. The change of structure of β-1,4-glucosidase and salicin in this system was investigated by UV ,FT IR and NMR. The activity and kinetics of β-1,4-glucosidase in this system were also studied. The results indicated that β-1,4-glucosidase follows the Michaelis-Menten kinetics in this reverse micelles, the v max is 1.475mg•min -1 ,11.8 times larger than that in aqueous, the Km is 0.389mg•mL -1 only 1/25 of that in aqueous. The reasons which cause this phenomenon are that the interaction at the interface of the reverse micelles changes the structure of β-1,4-glucosidase ,increases the concentration of salicin ,and that the -N +(CH 3) 3 in PC′ polar head attacks the oxygen of the glycoside bond of salicin .
- β-1，4-glucosidase,
- Phosphatidalcholine(PC),
- Reverse micelles,
- Solubilization
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