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Citation: Zhao Hai-Yan, Song Shi-Ying, Lin Zheng-Jiong, Du Xiao-Yan, Zhou Yuan-Cong. The Crystallization of Acidic Phospholipase A₂ from the Venom of Agkistrodon Blomhoffii Brevicaudus Covalently Inhibited by p-Bromo-phenacyl-bromide[J]. Acta Physico-Chimica Sinica, ;1997, 13(05): 449-451. doi: 10.3866/PKU.WHXB19970512 shu

The Crystallization of Acidic Phospholipase A₂ from the Venom of Agkistrodon Blomhoffii Brevicaudus Covalently Inhibited by p-Bromo-phenacyl-bromide

1.
National Laboratory of Biomacromolecules,Institute of Biophysics,Academia Sinica,Beijing 100101

Received Date: 21 August 1996
Available Online: 15 May 1997

The Crystals of acidic phospholipase A2 from the venom of Agkistrodon blomhoffii brevicaudus (i.e. Agkistrodon halys pallas) covalently modified with p-bromo-phenacylbromide were obtained by the method of hanging drop vapor diffusion. Crystallization droplet was composed of 0.06mol?L-1 Na(CH3)2AsO2 (pH=6.5), 24.8% (v/v) 1,4-butanediol, and 4.5mg?mL-1 protein. The crystal data are of a=b=82.82?., c=32.85?, space group P61. 8945 unique reflections with 1.93 ? resolution were measured on a Siemens area detector X-ray diffractometor, of which 8069 reflections having F0 >2σ(F). The experimental results show that the crystals are suitable to a structure analysis of high resolution.
- Phospolipase A₂,
- pBPB inhibition,
- Crystallization,
- X-ray diffraction
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The Crystallization of Acidic Phospholipase A2 from the Venom of Agkistrodon Blomhoffii Brevicaudus Covalently Inhibited by p-Bromo-phenacyl-bromide

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通讯作者: 陈斌, bchen63@163.com

The Crystallization of Acidic Phospholipase A₂ from the Venom of Agkistrodon Blomhoffii Brevicaudus Covalently Inhibited by p-Bromo-phenacyl-bromide