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Citation: Xiong Ya, Huang Su-Qiu, Wu Ding-Quan, Qu Song-Sheng. Studies on the Interactions of Two Kinds of Water-Soluble Porphyrins with DNA[J]. Acta Physico-Chimica Sinica, ;1996, 12(06): 543-546. doi: 10.3866/PKU.WHXB19960613 shu

Studies on the Interactions of Two Kinds of Water-Soluble Porphyrins with DNA

1.
Department of Chemistry,Wuhan University,Wuhan 430072

Received Date: 13 September 1995
Available Online: 15 June 1996

The interactions of two kinds of water-soluble porphyrins, Cu (NEAE) and H2PPS, with DNA have been investigated by microcalorimetry and UV spectroscopy. The experimental results show that Cu (NEAE) interact with dsDNA in the outside-bound mode, its binding number is 9～10 base pairs and its binding enthalpy △H＝-24.4kJ．mol-1. There are large enthalpy changes when Cu (NEAE) react with ssDNA. H2PPS can not react with both dsDNA and ssDAN, which demonstrats that the coulombic force is one of the important factors affecting the interactions of porphyrins with DNA.
- Porphyrin,
- DNA,
- Microcalorimetry,
- UV spectroscopy,
- Interaction
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