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Citation: Wang Bao-Huai, Zhang You-Min, Yang Ming, Miao Pei, Wang Kui. A Study of Intercalation Binding of Harmaline and Harmine to DNA by Microcalorimetry[J]. Acta Physico-Chimica Sinica, ;1994, 10(01): 82-86. doi: 10.3866/PKU.WHXB19940119 shu

A Study of Intercalation Binding of Harmaline and Harmine to DNA by Microcalorimetry

1.
Institute of Physical Chemistry, Peking University, Beijing 100871

Received Date: 16 May 1992
Available Online: 15 January 1994

The enthalpy changes for the binding of two alkaloids with the bioactivity to inhibit monoamine oxidase, harmaline(HL) and harmine(HM) to calf thymus DNA have been determined calorimetrically in a 0.01 mol·L~(-1) piperazine-N、N -bis(2-ethanesulfonic acid) buffer(pH=7.0) at 298.15 K by a MS-80 standard Calvet microcalorimeter. The results are △H_(298)(HL)=-10.7 kJ·mol~(-1), △H_(298)(HM)=-29.6 kJ·mol~(-1) respectively. According to the binding energy and thermokinetic curves from the experiments, the mechanism of intercalationm binding of two alkaloids to DNA has been discussed.
- Alkaloid,
- Anticancer,
- Intercalation,
- Enthalpy change,
- Thermogram
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