Home

Citation: LI Zhifei, GAO Ning. Cryo-EM Method for High-Resolution Structure Determination: A Brief Introduction to 2017 Nobel Prize in Chemistry[J]. University Chemistry, ;2018, 33(1): 1-6. doi: 10.3866/PKU.DXHX201711035 shu

Cryo-EM Method for High-Resolution Structure Determination: A Brief Introduction to 2017 Nobel Prize in Chemistry

LI Zhifei ,
GAO Ning

State Key Laboratory of Membrane Biology, Peking-Tsinghua Joint Center for Life Sciences, School of Life Sciences, Peking University, Beijing 100871, P. R. China

Received Date: 28 November 2017

The 2017 Nobel Prize in chemistry was awarded to Jacques Dubochet, Joachim Frank and Richard Henderson for their contribution to developing the cryo-electron microscopy (cryo-EM) method for high-resolution structure determination of biomolecules in solution. In recent years, cryo-EM is leading a revolution in structural biology, and becoming a major tool in studying the structure and function of biomolecular machines. Here, we briefly describe the method development of cryo-EM and the personal contributions of the three Nobel Laureates.
- Cryo-electron microscopy,
- Biomolecules,
- Structural biology,
- Nobel Prize in chemistry
1. [1]
2. [2]
3. [3]
4. [4]
5. [5]
6. [6]
7. [7]
8. [8]
9. [9]
10. [10]
11. [11]
12. [12]
13. [13]
14. [14]
15. [15]
16. [16]
17. [17]
18. [18]
19. [19]
20. [20]
21. [21]
22. [22]
23. [23]
24. [24]
1. [1]
  Shaohua Wu , Jianfeng Yan , Chunfa Xu , Yuanming Li , Kai Yang , Caixia Lin . Construction of the synthetic chemistry experiment course under the Chemistry “101 Plan”: instructional design and implementation of the L-proline-catalyzed asymmetric aldol condensation reaction experiment. University Chemistry, 2026, 41(6): 152-159. doi: 10.12461/PKU.DXHX202511100
2. [2]
  Zhe Ji , Guangxu Lan , Jiangnan Li , Sihai Yang . A New Protocol to Construct Molecular Materials: A Brief Introduction on the 2025 Nobel Prize in Chemistry. University Chemistry, 2025, 40(12): 70-77. doi: 10.12461/PKU.DXHX202511097
3. [3]
  Yang Liu , Peng Chen , Lei Liu . Chemistry “101 Plan”: Design and Construction of Chemical Biology Textbook. University Chemistry, 2024, 39(10): 45-51. doi: 10.12461/PKU.DXHX202407085
4. [4]
  Tianyu Feng , Guifang Jia , Peng Zou , Jun Huang , Zhanxia Lü , Zhen Gao , Chu Wang . Construction of the Chemistry Biology Experiment Course in the Chemistry “101 Program”. University Chemistry, 2024, 39(10): 69-77. doi: 10.12461/PKU.DXHX202409002
5. [5]
  Shangwen Luo , Jianguo Fang , Yanlong Yang , Shihui Dong . 化学生物学课程双语教学实践与探索. University Chemistry, 2025, 40(8): 124-129. doi: 10.12461/PKU.DXHX202410096
6. [6]
  Jing Li , Liang Wang , Yuxin Luan , Chan Cao , Chuanzheng Zhou . Teaching Reform and Reflections on Chemical Biology in the Context of “New Medical Science” Education. University Chemistry, 2026, 41(2): 140-145. doi: 10.12461/PKU.DXHX202502041
7. [7]
  Xinyi Hong , Tailing Xue , Zhou Xu , Enrong Xie , Mingkai Wu , Qingqing Wang , Lina Wu . Non-Site-Specific Fluorescent Labeling of Proteins as a Chemical Biology Experiment. University Chemistry, 2024, 39(4): 351-360. doi: 10.3866/PKU.DXHX202310010
8. [8]
  Fangfang Chen , Haiming Fan , Yan Li , Yuan He . 化学生物学专业多元化人才培养导向的课程体系优化探索. University Chemistry, 2025, 40(8): 92-99. doi: 10.12461/PKU.DXHX202409108
9. [9]
  Weiwei He , Cong Liu , Daichao Xu , Jing Wang , Min Sun . Case-driven, frontier-integrated, and mindset-building: instructional design for life processes from a chemical biology perspective. University Chemistry, 2026, 41(6): 64-71. doi: 10.12461/PKU.DXHX202508048
10. [10]
  Zhen Shen , Yi Wang , Chen Lin , Kin Shing Chan . 南京大学化学生物学专业本科生有机化学英文教学经验. University Chemistry, 2025, 40(6): 43-47. doi: 10.12461/PKU.DXHX202407083
11. [11]
  Xinyan Chen , Meng Xiao , Fei Cai , Junxian Guo , Tianfeng Chen , Li Ma . Transformation of Scientific Research Achievements Facilitating the Construction of Experimental Courses in Frontier Interdisciplinary Disciplines: A Case of “Comprehensive Experiments in Chemical Biology”. University Chemistry, 2025, 40(7): 373-379. doi: 10.12461/PKU.DXHX202408105
12. [12]
  Zhenhua Wang , Haoyang Feng , Xiaoyang Shao , Wenru Fan . Vitamins in Solid Propellants: Controlled Synthesis of Neutral Macromolecular Bonding Agents. University Chemistry, 2025, 40(4): 1-9. doi: 10.3866/PKU.DXHX202401007
13. [13]
  Yi RU , Tao MENG , Zhaoteng XUE , Dongsen MAO . Synergistic catalysis of Al distribution and pore structure in ZSM-5 zeolite for bioethanol-to-propylene. Chinese Journal of Inorganic Chemistry, 2026, 42(2): 247-262. doi: 10.11862/CJIC.20250255
14. [14]
  Haitang WANG , Yanni LING , Xiaqing MA , Yuxin CHEN , Rui ZHANG , Keyi WANG , Ying ZHANG , Wenmin WANG . Construction, crystal structures, and biological activities of two Ln^Ⅲ₃ complexes. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1474-1482. doi: 10.11862/CJIC.20240188
15. [15]
  Chengyan XU , Jiangnan YU , Yu LI , Xizhen PAN , Yinlin HOU , Wenmin WANG . Synthesis, structure, and bioactivity of a Ho₄ complex bridged by azide group. Chinese Journal of Inorganic Chemistry, 2026, 42(7): 1453-1462. doi: 10.11862/CJIC.20260033
16. [16]
  Jiamin Zhang , Zhen Fan , Jianzhong Du . Integrated Teaching Method Combining Domestic and International Perspectives: A Case Study on Cultivating Innovative Talents in Polymeric Biomaterials. University Chemistry, 2025, 40(7): 156-160. doi: 10.12461/PKU.DXHX202409131
17. [17]
  Zhaoyang Li , Haiyan Zhao , Yali Zhang , Yuan Zhang , Shiqiang Cui . Integration of Nobel Prize Achievements in Analytical Technology with College Instrumental Analysis Course. University Chemistry, 2025, 40(3): 269-276. doi: 10.12461/PKU.DXHX202405131
18. [18]
  Guojiao Wu , Yuzhou Wu , Weiming Yuan , Fangrui Zhong . Bridging chemistry and biology: innovative practices in organic chemistry teaching. University Chemistry, 2026, 41(6): 72-78. doi: 10.12461/PKU.DXHX202508064
19. [19]
  Xin MA , Ya SUN , Na SUN , Qian KANG , Jiajia ZHANG , Ruitao ZHU , Xiaoli GAO . A Tb₂ complex based on polydentate Schiff base: Crystal structure, fluorescence properties, and biological activity. Chinese Journal of Inorganic Chemistry, 2024, 40(7): 1347-1356. doi: 10.11862/CJIC.20230357
20. [20]
  Longfei MA , Fang SI , Congjie PAN , Xinhua WANG . Synthesis and crystal structure of extended tetrathiafulvalene derivatives and cupric bromide charge transfer complexes. Chinese Journal of Inorganic Chemistry, 2026, 42(6): 1229-1236. doi: 10.11862/CJIC.20250355

Get Citation

PDF

XML

Metrics

PDF Downloads(13)
Abstract views(1249)
HTML views(160)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Address：Zhongguancun North First Street 2,100190 Beijing, PR China Tel: +86-010-82449177-888

DownLoad: Full-Size Img PowerPoint

Return