Citation: ZHANG Miao, WANG Yuchen, MUHAMMAD Atif, CHEN Lijuan, WANG Yanmei. Application of mixed polymer brushes based on poly(2-methyl-2-oxazoline) and poly(acrylic acid) to on-line preconcentration of lysozyme by capillary electrophoresis[J]. Chinese Journal of Chromatography, ;2020, 38(9): 1085-1094. doi: 10.3724/SP.J.1123.2020.02027 shu

Application of mixed polymer brushes based on poly(2-methyl-2-oxazoline) and poly(acrylic acid) to on-line preconcentration of lysozyme by capillary electrophoresis

1.
Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei 230026, China;
2.
College of Materials and Chemical Engineering, West Anhui University, Lu'an 237012, China

Corresponding author: WANG Yanmei, wangyanm@ustc.edu.cn
Received Date: 26 February 2020

Fund Project: National Natural Science Foundation of China (No. 21674102).

A capillary coated with mixed polymer brushes that shows switchability toward lysozyme adsorption was developed. This capillary was applied for the on-line preconcentration of lysozyme by capillary electrophoresis (CE) in order to enhance the detection sensitivity. First, poly(2-methyl-2-oxazoline) (PMOXA) and poly(acrylic acid) (PAA) were synthesized by cationic ring-opening polymerization and reversible addition-fragmentation chain transfer (RAFT) polymerization, respectively. Then, glycidyl methacrylate (GMA) and PMOXA were used to prepare poly(2-methyl-2-oxazoline)-random-glycidyl methacrylate (PMOXA-r-GMA) via radical copolymerization, and poly(acrylic acid)-block-poly(glycidyl methacrylate) (PAA-b-PGMA) was obtained by the RAFT polymerization of GMA and PAA. A mixed solution of PMOXA-r-GMA and PAA-b-PGMA at a certain mass ratio was then injected into the capillary. Subsequent annealing provided capillary materials coated with mixed polymer brushes based on PMOXA and PAA. X-ray photoelectron spectroscopy (XPS) analysis was performed to determine the surface composition of the capillary raw materials. When the mass concentration of the mixed solution was 20 g/L and the mass ratio of PMOXA-r-GMA and PAA-b-PGMA was 1:1, the carboxyl content in the coating increased with increasing chain length of PAA. Fluorescein isothiocyanate-labeled lysozyme (FITC-lysozyme) adsorption assay demonstrated that the coated capillary had switchable properties for lysozyme adsorption upon pH and ionic strength (I) trigger. At pH 7 (I=10^-5mol/L), the capillary could adsorb a large amount of lysozyme, which could be released at pH 3 (I=10^-1mol/L). Subsequently, this coated capillary was applied to the on-line preconcentration of lysozyme by CE. The sensitivity enhancement factor was 17.69 when the chain length of PAA was 2.2 times that of PMOXA. The limit of detection could reach 8.7×10^-5g/L. On-line preconcentration of lysozyme was performed for five successive times on the same day and on five consecutive days. The intraday and interday relative standard deviations (RSDs) for the peak areas were 2.9% and 4.1%, respectively. The intraday and interday RSDs for the migration times were 0.9% and 2.1%, respectively. The developed method for the preparation of the coated capillary with good stability only needs one step in this work, and this research will supply a simple and effective way to analyze trace protein by CE.
- capillary electrophoresis (CE),
- on-line preconcentration,
- mixed polymer brushes,
- poly(2-methyl-2-oxazoline) (PMOXA),
- poly(acrylic acid) (PAA)
1. [1]
2. [2]
3. [3]
4. [4]
5. [5]
6. [6]
7. [7]
8. [8]
9. [9]
10. [10]
11. [11]
12. [12]
13. [13]
14. [14]
15. [15]
16. [16]
17. [17]
18. [18]
19. [19]
20. [20]
21. [21]
22. [22]
23. [23]
24. [24]
25. [25]
26. [26]
27. [27]
28. [28]
29. [29]
30. [30]
31. [31]
32. [32]
33. [33]
34. [34]
35. [35]
36. [36]
37. [37]
1. [1]
  Zijian Zhao , Yanxin Shi , Shicheng Li , Wenhong Ruan , Fang Zhu , Jijun Jiang . A New Exploration of the Preparation of Polyacrylic Acid by Free Radical Polymerization Based on the Concept of Green Chemistry. University Chemistry, 2024, 39(5): 315-324. doi: 10.3866/PKU.DXHX202311094
2. [2]
  Jingke LIU , Jia CHEN , Yingchao HAN . Nano hydroxyapatite stable suspension system: Preparation and cobalt adsorption performance. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1763-1774. doi: 10.11862/CJIC.20240060
3. [3]
  Peng XU , Shasha WANG , Nannan CHEN , Ao WANG , Dongmei YU . Preparation of three-layer magnetic composite Fe₃O₄@polyacrylic acid@ZiF-8 for efficient removal of malachite green in water. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 544-554. doi: 10.11862/CJIC.20230239
4. [4]
  Junke LIU , Kungui ZHENG , Wenjing SUN , Gaoyang BAI , Guodong BAI , Zuwei YIN , Yao ZHOU , Juntao LI . Preparation of modified high-nickel layered cathode with LiAlO₂/cyclopolyacrylonitrile dual-functional coating. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1461-1473. doi: 10.11862/CJIC.20240189
5. [5]
  Tianlong Zhang , Rongling Zhang , Hongsheng Tang , Yan Li , Hua Li . Online Monitoring and Mechanistic Analysis of 3,5-diamino-1,2,4-triazole (DAT) Synthesis via Raman Spectroscopy: A Recommendation for a Comprehensive Instrumental Analysis Experiment. University Chemistry, 2024, 39(6): 303-311. doi: 10.3866/PKU.DXHX202312006
6. [6]
  Anqiu LIU , Long LIN , Dezhi ZHANG , Junyu LEI , Kefeng WANG , Wei ZHANG , Junpeng ZHUANG , Haijun HAO . Synthesis, structures, and catalytic activity of aluminum and zinc complexes chelated by 2-((2,6-dimethylphenyl)amino)ethanolate. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 791-798. doi: 10.11862/CJIC.20230424
7. [7]
  Kangmin Wang , Liqiu Wan , Jingyu Wang , Chunlin Zhou , Ke Yang , Liang Zhou , Bijin Li . Multifunctional 2-(2′-hydroxyphenyl)benzoxazoles: Ready synthesis, mechanochromism, fluorescence imaging, and OLEDs. Chinese Chemical Letters, 2024, 35(10): 109554-. doi: 10.1016/j.cclet.2024.109554
8. [8]
  Yuexiang Liu , Xiangqiao Yang , Tong Lin , Guantian Yang , Xiaoyong Xu , Bubing Zeng , Zhong Li , Weiping Zhu , Xuhong Qian . Efficient continuous synthesis of 2-[3-(trifluoromethyl)phenyl]malonic acid, a key intermediate of Triflumezopyrim, coupling with esterification-condensation-hydrolysis. Chinese Chemical Letters, 2025, 36(1): 109747-. doi: 10.1016/j.cclet.2024.109747
9. [9]
  Zhaoyang WANG , Chun YANG , Yaoyao Song , Na HAN , Xiaomeng LIU , Qinglun WANG . Lanthanide(Ⅲ) complexes derived from 4′-(2-pyridyl)-2, 2′∶6′, 2″-terpyridine: Crystal structures, fluorescent and magnetic properties. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1442-1451. doi: 10.11862/CJIC.20240114
10. [10]
  Yue Li , Minghao Fan , Conghui Wang , Yanxun Li , Xiang Yu , Jun Ding , Lei Yan , Lele Qiu , Yongcai Zhang , Longlu Wang . 3D layer-by-layer amorphous MoS_x assembled from [Mo₃S₁₃]^2- clusters for efficient removal of tetracycline: Synergy of adsorption and photo-assisted PMS activation. Chinese Chemical Letters, 2024, 35(9): 109764-. doi: 10.1016/j.cclet.2024.109764
11. [11]
  Liyang ZHANG , Dongdong YANG , Ning LI , Yuanyu YANG , Qi MA . Crystal structures, luminescent properties and Hirshfeld surface analyses of three cadmium(Ⅱ) complexes based on 2-(3-(pyridin-2-yl)-1H-pyrazol-1-yl)benzoate. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1943-1952. doi: 10.11862/CJIC.20240079
12. [12]
  Zhuoyan Lv , Yangming Ding , Leilei Kang , Lin Li , Xiao Yan Liu , Aiqin Wang , Tao Zhang . Light-Enhanced Direct Epoxidation of Propylene by Molecular Oxygen over CuO_x/TiO₂ Catalyst. Acta Physico-Chimica Sinica, 2025, 41(4): 100038-. doi: 10.3866/PKU.WHXB202408015
13. [13]
  Zhening Lou , Quanxing Mao , Xiaogeng Feng , Lei Zhang , Xu Xu , Yuyang Zhang , Xueyan Liu , Hongling Kang , Dongyang Feng , Yongku Li . Practice of Implementing Blended Teaching in Shared Analytical Chemistry Course. University Chemistry, 2024, 39(2): 263-269. doi: 10.3866/PKU.DXHX202308089
14. [14]
  Ran Yu , Chen Hu , Ruili Guo , Ruonan Liu , Lixing Xia , Cenyu Yang , Jianglan Shui . 杂多酸H₃PW₁₂O₄₀高效催化MgH₂储氢. Acta Physico-Chimica Sinica, 2025, 41(1): 2308032-. doi: 10.3866/PKU.WHXB202308032
15. [15]
  Yan Liu , Yuexiang Zhu , Luhua Lai . Introduction to Blended and Small-Class Teaching in Structural Chemistry: Exploring the Structure and Properties of Crystals. University Chemistry, 2024, 39(3): 1-4. doi: 10.3866/PKU.DXHX202306084
16. [16]
  Huan Zhang , Linyu Pu , Wei Wang , Yatang Dai , Xu Huang . Curriculum Development and Blended Teaching Practice in the Graduate Course on Elemental Inorganic Chemistry. University Chemistry, 2024, 39(6): 166-173. doi: 10.3866/PKU.DXHX202402010
17. [17]
  Zhiguang Xu , Xuan Xu , Qiong Luo , Ganquan Wang , Bin Peng . Reform and Practice of Online and Offline Blended Teaching in Structural Chemistry Course. University Chemistry, 2024, 39(6): 195-200. doi: 10.3866/PKU.DXHX202310112
18. [18]
  Zhongxin YU , Wei SONG , Yang LIU , Yuxue DING , Fanhao MENG , Shuju WANG , Lixin YOU . Fluorescence sensing on chlortetracycline of a Zn-coordination polymer based on mixed ligands. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2415-2421. doi: 10.11862/CJIC.20240304
19. [19]
  Danqing Wu , Jiajun Liu , Tianyu Li , Dazhen Xu , Zhiwei Miao . Research Progress on the Simultaneous Construction of C—O and C—X Bonds via 1,2-Difunctionalization of Olefins through Radical Pathways. University Chemistry, 2024, 39(11): 146-157. doi: 10.12461/PKU.DXHX202403087
20. [20]
  Fangdong Hu , Xiaolei Jiang . Research and Practice of the “Integration of Theory and Practice Drives Innovation” Teaching Mode in Inorganic Chemistry under the Background of “Four New” Construction. University Chemistry, 2024, 39(11): 1-8. doi: 10.3866/PKU.DXHX202402013

Get Citation

PDF

XML

Metrics

PDF Downloads(9)
Abstract views(697)
HTML views(152)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142