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Citation: LI Ruonan, WANG Lijuan, ZHANG Dongtang, WANG Yanan, GUO Guangsheng, WANG Xiayan. Application of porous layer open tubular capillary columns with narrow inner diameter in nanoflow-high performance liquid chromatography for N-derivatized amino acid enantiomers[J]. Chinese Journal of Chromatography, ;2020, 38(3): 350-355. doi: 10.3724/SP.J.1123.2019.09002 shu

Application of porous layer open tubular capillary columns with narrow inner diameter in nanoflow-high performance liquid chromatography for N-derivatized amino acid enantiomers

Center of Excellence for Environmental Safety and Biological Effects, Beijing Key Laboratory for Green Catalysis and Separation, Department of Chemistry and Chemistry Engineering, Beijing University of Technology, Beijing 100124, China

Corresponding author: WANG Xiayan, xiayanwang@bjut.edu.cn
Received Date: 5 November 2019

Fund Project: National Natural Science Foundation of China (Nos. 21804005, 21625501)

Porous layer open tubular capillary columns with a narrow inner diameter (NPLOT) have potential in life science, especially in single-cell analysis. In this work, quinidine-based chiral NPLOT columns were prepared by in-situ thermal-initiated polymerization. The porous layer of the organic polymer was prepared with a 6 μm i. d capillary by in-situ thermal-initiated polymerization, following which the thickness and morphology of the NPLOT column were observed for different durations (3 h, 6 h, and 9 h) of polymerization. The thicknesses of the porous layer were 103±51 nm and 210±51 nm when polymerization was conducted for 3 h and 6 h, respectively. Subsequently, the columns prepared under 3 h of polymerization were applied in nanoflow-high performance liquid chromatography for the enantioseparation of N-derivatized amino acid enantiomers. Baseline separation was achieved in 2 min. The sample volume consumed was of the picoliter level, which would provide a means of research for single-cell analysis.
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