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Citation: YIN Chunyuan, SUN Mingqian, JIN Long, LIN Li, MIAO Lan, LIU Jianxun. Rat serum and heart tissue metabolomics in myocardial ischemia based on liquid chromatography-mass spectrometry[J]. Chinese Journal of Chromatography, ;2020, 38(2): 206-211. doi: 10.3724/SP.J.1123.2019.07019 shu

Rat serum and heart tissue metabolomics in myocardial ischemia based on liquid chromatography-mass spectrometry

YIN Chunyuan ^1,2 ,
SUN Mingqian ¹ ,
JIN Long ¹ ,
LIN Li ¹ ,
MIAO Lan ¹ ,
LIU Jianxun ^1,,

1.
Beijing Key Laboratory of Pharmacology of Chinese Materia Medica, Institute of Basic Medical Sciences of Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China;
2.
Beijing University of Chinese Medicine, Beijing 100029, China

Corresponding author: LIU Jianxun, liujx0324@sina.com
Received Date: 15 July 2019

Fund Project: National Basic Research Program of China ("973" Program) (No. 2015CB554405)

A rat model with isoproterenol (ISO)-induced myocardial ischemia was obtained. Liquid chromatography-mass spectrometry (LC-MS)was conducted on the serum and heart tissue metabolites of normal and model rats. Principal component analysis (PCA) and differentiation analysis of supervised partial least-squares method (PLS-DA) were applied to the metabolomics data for multidimensional statistical analysis, and the resulting biomarkers were screened. Compared with the normal group, 18 different endogenous metabolites in plasma and tissues were detected, including the products of arginine and proline metabolism; glycine, serine, and threonine metabolism; glutamine and glutamate metabolism; and taurine and hypotaurine metabolism. These metabolites can be used as important biomarkers for myocardial ischemia. The results of this study can help reveal the pathogenesis of myocardial ischemia and aid clinical diagnosis.
- liquid chromatography-mass spectrometry (LC-MS),
- biomarkers,
- metabolomics,
- myocardial ischemia
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