Citation: SUN Xiaoyu, MA Runtian, SHI Yanping. Recent advances in molecular imprinting technology for the separation and analysis of proteins[J]. Chinese Journal of Chromatography, ;2020, 38(1): 50-59. doi: 10.3724/SP.J.1123.2019.06017 shu

Recent advances in molecular imprinting technology for the separation and analysis of proteins

SUN Xiaoyu ^1,2 ,
MA Runtian ¹ ,
SHI Yanping ^1,,

1.
CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China;
2.
University of Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing 100049, China

Corresponding author: SHI Yanping, shiyp@licp.cas.cn
Received Date: 19 June 2019

Fund Project: National Natural Science Foundation of China (Nos. 21575150, 21775153, 21804135)

Proteins, which have complex structures and fall under diverse categories, are closely associated with different kinds of life activities. Majority of the proteins exist in low concentrations in complex biological samples, because of which their direct analysis without sample pretreatment is difficult. Thus, selective separation of the target proteins from complex biological samples is essential for efficient analysis. Molecularly imprinted polymers, containing numerous imprinted cavities that are complementary with the templates in terms of shape, size, and functional groups, have great potential for use in the selective recognition and separation of the target proteins from complex biological samples. However, the large size, structural flexibility, and complex structures of proteins hinder their efficient separation and analysis by conventional molecular imprinting technology. In this review, several novel molecular imprinting techniques, including surface imprinting, epitope imprinting, and metal chelate imprinting, are introduced. The emergence of these novel technologies has contributed to advances in protein analysis. The applications of these molecular imprinting techniques to the separation and analysis of proteins in the last three years are summarized herein. Finally, the promising future of molecular imprinting techniques in the area of proteins is prospected.
- molecular imprinting,
- proteins,
- separation and determination,
- review
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