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Citation: ZHAO Yameng, WANG Min, ZHANG Lingyi, LU Yuhao, ZHANG Weibing. Preparation of magnetic nanoparticles based on MIL-100(Fe) and its application in the enrichment of endogenous peptides[J]. Chinese Journal of Chromatography, ;2019, 37(8): 831-835. doi: 10.3724/SP.J.1123.2019.04048 shu

Preparation of magnetic nanoparticles based on MIL-100(Fe) and its application in the enrichment of endogenous peptides

School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China

Received Date: 30 April 2019

Fund Project: National Natural Science Foundation of China (Nos. 21475044, 51472121).

Core-shell magnetic nanoparticles, Fe₃O₄@MIL-100(Fe), were synthesized by layer-by-layer self-assembly. The final material, Fe₃O₄@MC, was obtained after high temperature calcination. Fe₃O₄@MC magnetic nanoparticles exhibited large pore size (17.78 nm) and high carbon content (6.79%). Thirty-three peptides of bovine serum albumin (BSA) digest were enriched by Fe₃O₄@MC magnetic nanomaterial. Fe₃O₄@MC magnetic nanomaterial also showed high selectivity for peptides when the mass ratio of BSA digest to BSA was 1:400. In addition, the nanomaterial material exhibited excellent enrichment performance for the endogenous peptides of human serum.
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