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Citation: LÜ Jiale, LIU Zhengcai, YAO Minna, LIN Yuandi. Determination of four protease inhibitors in chicken by ultra performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, ;2020, 38(2): 212-217. doi: 10.3724/SP.J.1123.2019.04047 shu

Determination of four protease inhibitors in chicken by ultra performance liquid chromatography-tandem mass spectrometry

LÜ Jiale ¹ ,
LIU Zhengcai ^2,, ,
YAO Minna ^1,, ,
LIN Yuandi ²

1.
Food Science College of Fujian Agriculture and Forestry University, Fuzhou 350002, China;
2.
Technology center of Fuzhou Customs District, Fuzhou 350001, China

Corresponding author: LIU Zhengcai, YAO Minna,
Received Date: 29 April 2019

Fund Project: Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China (No. 2014IK110)

A method was developed for the determination of four protease inhibitors (saquinavir, ritonavir, nelfinavir and indinavir) in chicken using ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The samples were extracted by shaking with 30% (v/v) acetonitrile aqueous solution (containing 1% (v/v) trichloroacetic acid), and purified by using mixed-mode cationic-exchanger (MCX) cartridges. The samples were separated on a Luna^® C8 column (150 mm×2 mm, 3 μ m) using 0.2% (v/v) formic acid aqueous solution (containing 5 mmol/L ammonium acetate) and acetonitrile as the mobile phases with gradient elution. The determination was carried out by using an electrospray ion source in the positive and multiple-reaction monitoring (MRM) modes. The calibration curves showed good linearities in the range of 0.1-20.0 μ g/L, and the correlation coefficients (r²) were greater than 0.99. The limits of quantification (LOQs, S/N=10) of the four protease inhibitors varied from 0.20 μ g/kg to 0.90 μ g/kg. At the spiked levels of 1.0, 2.0, and 10.0 μ g/kg, the average recoveries of the four protease inhibitors were ranging from 69.0% to 106.0%. The intra-day and inter-day relative standard deviations (RSDs) were 2.2%-13.8% (n=6) and 3.6%-14.6% (n=3), respectively. The method is simple, efficient, sensitive and accurate, and it can be used to detect residues of saquinavir, ritonavir, nelfinavir and indinavir in chicken.
- ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS),
- solid phase extraction (SPE),
- saquinavir,
- ritonavir,
- nelfinavir,
- indinavir,
- chicken
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