Citation: HUO Chunhui, LI Yinghua, QIAO Zhi, SHANG Zhi, CAO Chengxi, HONG Yang, XIAO Hua. Proteomics analysis of serum exosomes and its application in osteoporosis[J]. Chinese Journal of Chromatography, ;2019, 37(8): 863-871. doi: 10.3724/SP.J.1123.2019.04022 shu

Proteomics analysis of serum exosomes and its application in osteoporosis

HUO Chunhui ^1, ,
LI Yinghua ² ,
QIAO Zhi ¹ ,
SHANG Zhi ¹ ,
CAO Chengxi ³ ,
HONG Yang ² ,
XIAO Hua ¹

1.
State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China;
2.
The Fifth People's Hospital of Shanghai, Fudan University, Shanghai 200240, China;
3.
School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China

Received Date: 12 April 2019

Fund Project: National Key Research and Development Program (No. 2017YFC1200204)

Exosomes are tiny vesicles secreted by cells, can be important mediators of cell-to-cell communication, and play unique roles in disease diagnosis and treatment. Osteoporosis is a metabolic bone disease with high incidence in the elderly, characterized by low bone mineral density and deterioration of bone microstructure. Highly specific diagnostic methods capable of identifying early-stage osteoporosis are urgently needed. In this study, serum exosomes were comprehensively enriched and characterized. In total, 179 exosomal proteins were identified using liquid chromatography-mass spectrometry, most of which are involved in important biological processes such as defense and immune responses. Through label free quantification of serum exosomes, 188, 224, and 185 proteins were identified in the normal, osteopenia, and osteoporosis groups, respectively. Quantitative results also showed that 17 proteins were significantly (p <0.05) dysregulated in the osteoporosis and osteopenia groups, including Integrin β 3, Integrin α 2 β 1, Talin 1, and Gelsolin. This study provides potential molecular markers for osteoporosis studs and will contributes to the elucidating the pathogenesis of osteoporosis.
- proteomics,
- biomarkers,
- exosomes,
- osteoporosis,
- osteopenia
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