Citation: GAO Jie, WU Qi, CHEN Lixiao, LI Hui, DONG Shuqing, LUO Guoying, ZHANG Shusheng, QIU Hongdeng, ZHAO Liang. Preparation of reversed-phase/hydrophilic chromatographic stationary phase based on dialdehyde microcrystalline cellulose-functionalized C₁₈[J]. Chinese Journal of Chromatography, ;2020, 38(4): 414-423. doi: 10.3724/SP.J.1123.2019.03009 shu

Preparation of reversed-phase/hydrophilic chromatographic stationary phase based on dialdehyde microcrystalline cellulose-functionalized C₁₈

GAO Jie ^1,2 ,
WU Qi ^1,2 ,
CHEN Lixiao ^1,2 ,
LI Hui ¹ ,
DONG Shuqing ¹ ,
LUO Guoying ¹ ,
ZHANG Shusheng ³ ,
QIU Hongdeng ^1,, ,
ZHAO Liang ^1,,

1.
Key Laboratory of Chemistry of Northwestern Plant Resources, Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China;
2.
University of Chinese Academy of Sciences, Beijing 100049, China;
3.
College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, China

Corresponding author: QIU Hongdeng, ZHAO Liang,
Received Date: 11 March 2019

Fund Project: National Natural Science Foundation of China (Nos. 21702210, 21675163).

A novel reversed/hydrophilic chromatographic stationary phase based on dialdehyde microcrystalline cellulose (DMCC)-functionalized C₁₈ was prepared by covalent bonding between the amino groups of octadecylamine with the aldehyde groups of DMCC, which was used in reversed-phase liquid chromatography (RPLC) and hydrophilic interaction liquid chromatography (HILIC) modes. The prepared DMCC-functionalized C₁₈ modified silica (C₁₈-DMCC/SiO₂) stationary phase exhibited good hydrophobic selectivity and aromatic selectivity by separating alkylbenzenes and polycyclic aromatic hydrocarbons (PAHs) in the RPLC mode. Polar compounds, including anilines, phenols, and glycosides were chosen as analytes to evaluate the polar selectivity of this column in the RPLC mode, and the evaluation results were satisfactory compared with the commercial C₁₈ column. Nucleobases were used for evaluating the hydrophilic interaction liquid chromatography performance of the C₁₈-DMCC/SiO₂ column. By investigating the impact of organic solvent content on the retention, it could be found that this new stationary phase had the typical characteristics of reversed-phase/hydrophilic chromatography.
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Preparation of reversed-phase/hydrophilic chromatographic stationary phase based on dialdehyde microcrystalline cellulose-functionalized C18

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Preparation of reversed-phase/hydrophilic chromatographic stationary phase based on dialdehyde microcrystalline cellulose-functionalized C₁₈