Citation: FA Yun, ZHANG Jinling, ZHAO Haijie, LIU Huizhou. Progress in separation and purification of nattokinase and its enzyme activity determination[J]. Chinese Journal of Chromatography, ;2019, 37(3): 274-278. doi: 10.3724/SP.J.1123.2018.11010 shu

Progress in separation and purification of nattokinase and its enzyme activity determination

FA Yun ^1,2, ,
ZHANG Jinling ³ ,
ZHAO Haijie ² ,
LIU Huizhou ^1,2

1.
University of Chinese Academy of Sciences, Beijing 100049, China;
2.
Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China;
3.
Weifang Customs, Weifang 261041, China

Received Date: 8 November 2018

Fund Project: National Natural Science Foundation of China (No. 21405166).

In this paper, the separation and purification of nattokinase have been reviewed by primarily focusing on solvent precipitation, adsorption column chromatography, magnetic microspheres, expanded bed, reverse micelle, and three-phase separation methods. The different methods for determination of the enzyme activity have been discussed and compared. Finally, the feasibility that the nucleic acid-based identification techniques can be used for nattokinase purification and enzyme activity assay has been proposed.
- separation purification,
- nattokinase,
- enzyme activity,
- review
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