Citation: CHEUK Man-Yung, LO Yun-Chuen, POON Wing-Tat. Determination of urine catecholamines and metanephrines by reversed-phase liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, ;2017, 35(10): 1042-1047. doi: 10.3724/SP.J.1123.2017.06011 shu

Determination of urine catecholamines and metanephrines by reversed-phase liquid chromatography-tandem mass spectrometry

Department of Pathology, Pamela Youde Nethersole Eastern Hospital, Hong Kong 999077, China

Received Date: 7 June 2017

The measurement of urine catecholamine and metanephrine concentrations is important for biochemical screening and diagnosis of pheochromocytoma. The goal of this work was to develop a simple liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for determining catecholamines and metanephrines in urine to replace an existing liquid chromatographic method using electrochemical detection. Urine samples were prepared using Oasis weak-cation-exchange cartridges. The eluate was analyzed on an Agilent ZORBAX Eclipse Plus Phenyl-Hexyl column in 3 min. Adrenaline, noradrenaline, dopamine, metanephrine, normetanephrine, and their deuterated internal standards were monitored in positive electrospray ionization mode by multiple reaction monitoring (MRM). No evidence of ion suppression was observed. The assay was linear up to 5μmol/L for adrenaline, 5μmol/L for noradrenaline, 6.1μmol/L for dopamine, 5.6μmol/L for metanephrine, and 34.6μmol/L for normetanephrine, with lower limits of quantification of 5, 5, 12, 6 and 7 nmol/L, respectively. The intra-day and inter-day precisions for all analytes ranged from 0.59% to 4.64% and 1.98% to 4.80%, respectively. External quality assurance samples were assayed and showed excellent agreement with the target values. This simple method provides an improved assay for determining urine catecholamines and metanephrines.
- liquid chromatography-tandem mass spectrometry (LC-MS/MS),
- metanephrines,
- catecholamines,
- pheochromocytoma,
- urine
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沈阳化工大学材料科学与工程学院沈阳 110142