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Citation: WANG Sulan, GAO Huaping, ZHANG Jing, YE Xiang. Stable isotope labeling and parallel reaction monitoring-based proteomic quantification for biomarker screening and validation of hepatocellular carcinoma[J]. Chinese Journal of Chromatography, ;2017, 35(9): 934-940. doi: 10.3724/SP.J.1123.2017.05022 shu

Stable isotope labeling and parallel reaction monitoring-based proteomic quantification for biomarker screening and validation of hepatocellular carcinoma

Sir Run Run Shaw Hospital Xiasha Branch, Zhejiang University, Hangzhou 310018, China

Received Date: 30 May 2017

Liver cancer is the fifth most common cancer with extremely low five year survival rate. Early diagnosis is of great importance for cancer therapy. In this work, stable isotope labeling-based relative quantitative proteomics and parallel reaction monitoring-based target proteomics were combined for cancer biomarker screening and validation. By using this strategy, 70 significantly changed proteins in hepatocellular carcinoma tissues were obtained, among which seven proteins were further validated. The validated proteins contain the clinically used hepatocellular carcinoma (HCC) biomarker alpha-fetoprotein (AFP) and the reported biomarker candidates Heat shock protein HSP 90-beta (HSP90), fatty acid-binding protein, epidermal (FABP5) and alcohol dehydrogenase 4 (ADH4), which demonstrated the robustness of the strategy. The proteins identified in this work could be benefit for further HCC biomarker screening and clinical validation. Moreover, this strategy could be further applied to other cancer types.
- proteomics,
- stable isotope labeling,
- parallel reaction monitoring,
- hepatocellular carcinoma (HCC)
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