Citation: WANG Hongwei, LIU Zhongshan, PENG Xiaojun, OU Junjie, YE Mingliang. Fabrication of acylsemicarbazide-based porous organic polymer for selective enrichment of glycopeptides[J]. Chinese Journal of Chromatography, ;2017, 35(7): 688-695. doi: 10.3724/SP.J.1123.2017.05014 shu

Fabrication of acylsemicarbazide-based porous organic polymer for selective enrichment of glycopeptides

1.
Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China;
2.
State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, China

Received Date: 17 May 2017

Fund Project: China State Key Research Grant (No.2016YFA0501402)

An acylsemicarbazide-based porous organic polymer (POP) was facilely prepared by the polymerization of benzene-1,3,5-tricarbohydrazide (BTZ) and 1,4-phenylene diisocyanate (PDI). The physical properties of as-synthesized material were characterized by Fourier-transform infrared spectroscopy (FT-IR), solid-state cross polarisation magic angle spinning carbon-13 nuclear magnetic resonance (CP-MAS ¹³C NMR), nitrogen adsorption/desorption measurement, water contact angle and so on. The specific surface area was 166 m²/g, and the water contact angle was 46.4°, exhibiting hydrophilic property of the porous material. Thus, POP-1 was tried to be used for glycopeptides enrichment from tryptic digest of standard protein and complex biosamples in hydrophilic mode. The 19 typical N-linked glycopeptides were identified from 500 fmol tryptic digest of immunoglobulin G (IgG) from human serum by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF/MS) analysis, meanwhile, 1919 unique glycopeptides with 1350 N-glycosylation sites from 605 different N-linked glycoproteins were identified from 100 μ g mouse liver tryptic digest by capillary liquid chromatography (cLC)-MS/MS analysis. These results demonstrated the great potential of POP-1 for glycoproteome analysis.
- acylsemicarbazide,
- porous organic polymer,
- benzene-1,3,5-tricarbohydrazide (BTZ),
- 1,4-phenylene diisocyanate (PDI),
- glycopeptide enrichment,
- hydrophilic
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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Fabrication of acylsemicarbazide-based porous organic polymer for selective enrichment of glycopeptides

南开大学师唯/华北电力大学（保定）刘景维：二维配位聚合物中有序的亲锂冠醚位点用于无枝晶锂沉积

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通讯作者: 陈斌, bchen63@163.com