Citation: LONG Zhen, GAMACHE Paul, GUO Zhimou, PAN Yuanyuan, ZHANG Yanhai, LIU Xiaoda, JIN Yan, LIU Lüye, LIANG Lina, LI Renyong. A highly sensitive high performance liquid chromatography-electrochemical detection method for the determination of five phenolic compounds from Salvia miltiorrhiza[J]. Chinese Journal of Chromatography, ;2017, 35(8): 897-905. doi: 10.3724/SP.J.1123.2017.03038 shu

A highly sensitive high performance liquid chromatography-electrochemical detection method for the determination of five phenolic compounds from Salvia miltiorrhiza

1.
Thermo Fisher Scientific Corporation, Beijing 100080, China;
2.
Thermo Fisher Scientific Corporation, Chelmsford 01824, USA;
3.
Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China

Received Date: 29 March 2017

The determination of antioxidants continues to be interested, since the oxidative damage is thought to be one of the main mechanisms involved in nearly all chronic renal pathologies. A highly sensitive high performance liquid chromatography-electrochemical detection (HPLC-ECD) method was developed for evaluating the antioxidant properties of Salvia miltiorrhiza (Dan Shen). The method was optimized with respect to selectivity and sensitivity. Chromatographic conditions, including mobile phase pH value, buffer concentration, buffer type, organic solvent type, gradient profile and flow rate, were systematically investigated. Low pH value (2.8), low buffer concentration (20 mmol/L NaH₂PO₄), a shallow water-acetonitrile gradient, and a flow rate of 0.2 mL/min were the determined optimal conditions for the quantitative analysis of aimed five antioxidants from 14 batches of Dan Shen samples. The described method provided a good recovery (>95%), a very wide linear range (up to 10⁴ for all analytes), a good precision (RSDs<4.01%), and a high sensitivity (LOQ of caffeic acid, 1.5 μ g/L). Compared with UV detection, the described ECD method was also more effective for evaluating the antioxidant properties of Dan Shen, as it provided highly selective detection of electro-active antioxidants
- high performance liquid chromatography-electrochemical detection (HPLC-ECD),
- gradient elution,
- phenolic compound,
- antioxidant,
- herbal medicine (HM)
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沈阳化工大学材料科学与工程学院沈阳 110142