Citation: DENG Linlin, ZHEN Qianna, GAO Jieying, JIN Mingchao, DING Min, XU Biao. Simultaneous determination of plasma indole and skatole in pregnant women with hepatitis B virus infection by high performance liquid chromatography[J]. Chinese Journal of Chromatography, ;2017, 35(7): 735-740. doi: 10.3724/SP.J.1123.2017.03025 shu

Simultaneous determination of plasma indole and skatole in pregnant women with hepatitis B virus infection by high performance liquid chromatography

DENG Linlin ^1, ,
ZHEN Qianna ² ,
GAO Jieying ³ ,
JIN Mingchao ¹ ,
DING Min ¹ ,
XU Biao ⁴

1.
Key Laboratory of Clinical Laboratory Diagnostics(Ministry of Education of China), College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China;
2.
Department of Endocrinology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China;
3.
School of Public Health and Management, Chongqing Medical University, Chongqing 400016, China;
4.
Department of Clinical Laboratory, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China

Received Date: 21 March 2017

Fund Project: National Natural Science Foundation of China-Youth Foundation (No.81200294).

A high performance liquid chromatographic (HPLC) method with fluorescence detection (FD) was established to simultaneously determine plasma indole and skatole. Plasma samples were pretreated by liquid-liquid extraction. Chromatographic separation was accomplished on a Shim-Pack VP-ODS column (150 mm×4. 6 mm, 4.6 μ m) using an isocratic mixture of 15 mmol/L sodium dihydrogen phosphate solution and methanol (40:60, v/v). The fluorescence excitation and emission wavelengths were 274 nm and 340 nm, respectively. The linear ranges were 2.22-88.89 μ g/L for indole and 1.11-44.44 μ g/L for skatole. The detection limits were 0.11 μ g/L and 0.06 μ g/L for indole and skatole, respectively. The recoveries were in the range of 95.5%-112.3% with the relative standard deviations less than 6.8%. The method was successfully applied to the analysis of plasma from healthy pregnant women (n=46) and pregnant women with hepatitis B virus (HBV) infection (n=29). The results showed that plasma indole and skatole levels were significantly different between two groups. In pregnant women with HBV infection, the concentrations of indolic compounds were positively associated with transaminase levels.
- high performance liquid chromatography (HPLC),
- indole,
- skatole,
- pregnant women with hepatitis B virus infection,
- liver function
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