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Citation: ZHANG Wenhui, CAI Chunxue, WANG Jing, MAO Zhen, LI Yueqiu, DING Liang, SHEN Shigang, DOU Haiyang. Application of asymmetrical flow field-flow fractionation for size characterization of low density lipoprotein in egg yolk plasma[J]. Chinese Journal of Chromatography, ;2017, 35(8): 855-859. doi: 10.3724/SP.J.1123.2017.03001 shu

Application of asymmetrical flow field-flow fractionation for size characterization of low density lipoprotein in egg yolk plasma

ZHANG Wenhui ^1,2, ,
CAI Chunxue ¹ ,
WANG Jing ² ,
MAO Zhen ¹ ,
LI Yueqiu ³ ,
DING Liang ¹ ,
SHEN Shigang ² ,
DOU Haiyang ^1,2

1.
College of Medicine, Hebei University, Baoding 071000, China;
2.
College of Chemistry and Environmental Science, Hebei University, Baoding 071002, China;
3.
Medical Experimental Center of Hebei University, Baoding 071000, China

Received Date: 1 March 2017

Fund Project: Natural Science Foundation of Hebei Province (No. B2016201002)

Home-made asymmetrical flow field-flow fractionation (AF4) system, online coupled with ultraviolet/visible (UV/Vis) detector was employed for the separation and size characterization of low density lipoprotein (LDL) in egg yolk plasma. At close to natural condition of egg yolk, the effects of cross flow rate, sample loading, and type of membrane on the size distribution of LDL were investigated. Under the optimal operation conditions, AF4-UV/Vis provides the size distribution of LDL. Moreover, the precision of AF4-UV/Vis method proposed in this work for the analysis of LDL in egg yolk plasma was evaluated. The intra-day precisions were 1.3% and 1.9% (n=7) and the inter-day precisions were 2.4% and 2.3% (n=7) for the elution peak height and elution peak area of LDL, respectively. Results reveal that AF4-UV/Vis is a useful tool for the separation and size characterization of LDL in egg yolk plasma.
- asymmetrical flow field-flow fractionation (AF4),
- low density lipoprotein (LDL),
- size characterization,
- egg yolk plasma
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