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Citation: FAN Zhichen, HAN Zheng, GUO Wenbo, ZHAO Zhihui. Simultaneous determination of 30 mycotoxins in different feed products by ultra high performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, ;2017, 35(6): 627-633. doi: 10.3724/SP.J.1123.2017.02010 shu

Simultaneous determination of 30 mycotoxins in different feed products by ultra high performance liquid chromatography-tandem mass spectrometry

1.
College of Food Science & Technology, Shanghai Ocean University, Shanghai 201306, China;
2.
Institute for Agro- Food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China

Received Date: 12 February 2017

Fund Project: Shanghai Agriculture Applied Technology Development Program of China (No. G20140302).

A method for the simultaneous determination of 30 mycotoxins in different feed products was developed using a QuEChERS pretreatment procedure coupled with ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). The samples were extracted with 5 mL water and 5 mL acetonitrile containing 1% (v/v) formic acid. An aliquot of the supernatant was dried by nitrogen gas, re-dissolved by 1mL water containing 5 mmol/L ammonium acetate-acetonitrile (80:20, v/v), and analyzed by UHPLC-MS/MS. Matrix-matched calibration curves and internal standards were used for accurate quantification. Satisfactory recoveries at low, medium and high spiked levels were ranged from 72.0% to 118.4% (n=5). Good linear relationships were obtained, the correlation coefficients (r²) were greater than 0.99. The limits of detection (LODs, S/N=3) and the limits of quantification (LOQs, S/N=10) ranged from 0.7 μg/L to 20 μg/L and from 2 μg/L to 50 μg/L, respectively. The proposed method is simple, rapid and valuable, which can be a powerful tool for quantitative analysis of the 30 mycotoxins in premixed feed, concentrated feed and formula feed products.
- ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS),
- QuEChERS,
- mycotoxins,
- feed
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