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Citation: XU Xiaomin, ZHANG Jingshun, CAI Zengxuan, ZHANG Jing, HUANG Baifen, XU Jiaojiao, CHEN Qing. Determination of amanitins and phallotoxins in wild mushrooms by online liquid chromatography-diode array detector-tandem mass spectrometry[J]. Chinese Journal of Chromatography, ;2017, 35(6): 613-619. doi: 10.3724/SP.J.1123.2017.02008 shu

Determination of amanitins and phallotoxins in wild mushrooms by online liquid chromatography-diode array detector-tandem mass spectrometry

Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China

Received Date: 9 February 2017

Fund Project: Science Research Foundation Program of Zhejiang Provincial Medicine and Health (No. 2015DTA006).

A fast and wide linear range method was established for the determination of mushroom toxins amanitins (α-amanitin,β-amanitin and γ-amanitin) and phallotoxins (phallacidin and phalloidin) in wild mushrooms by online liquid chromatography-diode array detector-tandem mass spectrometry (LC-DAD-MS/MS). The mushroom toxins were extracted with methanol, and diluted with water. The extracts were separated on an XBridge^TM BEH C₁₈ column (150 mm×3.0 mm, 2.5 μm) under pH 10.7, measured by DAD and then analyzed by MS/MS. Basic mobile phase conditions were applied to improve the ionization efficiency of hydrogen ion adducts. The baseline separation of the analytes was obtained within 15 min. The limits of detection (LODs) of the sample matrix were 0.005-0.02 mg/kg. The toxins were quantified by the results measured by MS/MS when the toxin contents less than 2 mg/kg, and quantified by the results obtained from DAD when the contents more than 2 mg/kg. The linear range was 0.05-500 mg/kg for the whole method in one injection. The method was successfully applied to the analyses of amanitins and phallotoxins in Lepiota brunneoincarnata and white Amanita.
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