Citation: LAI Guoyin, WANG Lijuan, LU He, ZENG Qiumei, XU Dunming, WANG Xiaoqin, LIN Liyi, ZHANG Zhigang, LÜ Meiling. Qualitative analysis of phenolic compounds in Camellia Sinensis seeds by ultra-high performance liquid chromatography combined with quadrupole time-of-flight mass spectrometry[J]. Chinese Journal of Chromatography, ;2017, 35(5): 502-508. doi: 10.3724/SP.J.1123.2016.11010 shu

Qualitative analysis of phenolic compounds in Camellia Sinensis seeds by ultra-high performance liquid chromatography combined with quadrupole time-of-flight mass spectrometry

1.
Xiamen Entry-Exit Inspection and Quarantine Bureau, Xiamen 361026, China;
2.
College of Chemical Engineering, Institute of Oil and Natural Product, Huaqiao University, Xiamen 361021, China;
3.
Agilent Technologies(China) Co. Ltd, Beijing 100102, China

Received Date: 12 November 2016

Fund Project: Natural Science Foundation of Fujian Province (No. 2016J05045)

Phenol compounds from some plants are considered as the natural anti-oxidants and have been demonstrated with the beneficial biological activities. In this work, the ultra-high performance liquid chromatography (UHPLC) combined with quadrupole time-of-flight mass spectrometry (Q-TOF/MS) was applied to qualitatively determine the phenolic compounds in Camellia Sinensis seeds. The seed sample was extracted using ethanol aqueous solution and further subjected to the UHPLC separation with the reversed phase C18 column as the stationary phase and the acidified acetonitrile/water as the binary mobile phases. The eluent from the column was directed to Q-TOF/MS for compound identification. Based on a number of reports on the chemical components in Camellia Sinensis and the related plants, a customized compound database was initially created including the name and formula of the previously reported 106 phenol compounds. The acquired MS scan data were searched against the customized phenol compound database. The retrieved compounds were then subjected to MS/MS scan, and the obtained fragment ions were applied to further determine the possible structures. A total of 24 phenolic compounds were tentatively identified, including 13 phenolic acids, 4 catechins, and 7 flavonoids, which were characteristic in the ethanol extract according to the available references and the relevant structural information of the molecular and fragment ions. These compounds were further confirmed by matching against the standard compounds in retention times and fragment ions. The results demonstrated that the UHPLC-Q-TOF/MS with the high mass accuracy and the high resolution is a reliable technology for rapid qualitative identification of the phenolic compounds in Camellia Sinensis seeds, promoting the discovery and identification of the new compounds.
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沈阳化工大学材料科学与工程学院沈阳 110142