Citation: GENG Shan, WANG Juanqiang, XI Xingjun, CHU Qiao, DONG Genlai, MA Xiaomeng, ZHANG Huiwen, WEI Yun. Separation and purification of flavonoids from Mikania micrantha by macroporous resin combined with high speed countercurrent chromatography[J]. Chinese Journal of Chromatography, ;2017, 35(3): 302-307. doi: 10.3724/SP.J.1123.2016.10022 shu

Separation and purification of flavonoids from Mikania micrantha by macroporous resin combined with high speed countercurrent chromatography

GENG Shan ^1, ,
WANG Juanqiang ¹ ,
XI Xingjun ² ,
CHU Qiao ² ,
DONG Genlai ² ,
MA Xiaomeng ¹ ,
ZHANG Huiwen ³ ,
WEI Yun ^1,3

1.
State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China;
2.
China National Institute of Standardization, Beijing 100191, China;
3.
School of Sciences, Beijing University of Chemical Technology, Beijing 100029, China

Received Date: 12 October 2016

Fund Project: Program for New Century Excellent Talents in University (No. NCET-11-0563) Beijing Nova Program Interdisciplinary Cooperation Project (No. Z161100004916045).National Natural Science Foundation of China (No. 21075007)

The present study established a method for the separation and purification of four flavonoids quercetin-3-O-rutinoside, kaempferol-3-O-rutinoside, luteoloside and astragalin from Mikania micrantha by using macroporous resin combined with high speed countercurrent chromatography (HSCCC). Macroporous resin was AB-8 and the eluent was 50% (v/v) ethanol. A two-phase solvent system composed of n-butanol/acetic acid/water (4:1:5, v/v) was used in HSCCC. Quercetin-3-O-rutinoside, kaempferol-3-O-rutinoside, luteoloside and astragalin with the purity of 90.12%, 98.55%, 98.33% and 99.23%, respectively, were successfully separated from Mikania micrantha by HSCCC using the lower phase of this solvent system as mobile phase in one run. This study pave a simple and efficient method that could be used in the following separation of flavonoids from invasive plants.
- high speed countercurrent chromatography (HSCCC),
- macroporous resin,
- separation,
- flavonoids,
- Mikania micrantha (M. micrantha)
1. [1]
2. [2]
3. [3]
4. [4]
5. [5]
6. [6]
7. [7]
8. [8]
9. [9]
10. [10]
11. [11]
12. [12]
13. [13]
14. [14]
15. [15]
16. [16]
17. [17]
18. [18]
19. [19]
20. [20]
21. [21]
22. [22]
23. [23]
24. [24]
25. [25]
26. [26]
1. [1]
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