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Citation: WANG Jiaojiao, YANG Xiaxia, LIU Xiaoyan, ZHANG Haixia. Synthesis of functional mesoporous silica and selective adsorption of surface-exposed histidine proteins[J]. Chinese Journal of Chromatography, ;2017, 35(3): 255-259. doi: 10.3724/SP.J.1123.2016.10021 shu

Synthesis of functional mesoporous silica and selective adsorption of surface-exposed histidine proteins

1.
College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China;
2.
Zhongwei High Technology Research Institute of Lanzhou University, Zhongwei 755000, China

Received Date: 9 October 2016

Fund Project: National Natural Science Foundation of China (Nos. 21105039, 21375052).

The Cu²⁺immobilized mesoporous silica particles (PPOSS-IDA-Cu²⁺) were fabricated. The prepared materials were applied to the highly specific separation of surface-exposed histidine proteins, based on immobilized metal ion affinity chromatography. The adsorption experiments of bovine serum albumin, myoglobin, lysozyme and bovine hemoglobin (BHb) exhibited that PPOSS-IDA-Cu²⁺ had excellent performance in the separation of protein BHb. The maximum binding capacity of the proposed materials for BHb was 3150 mg/g. The results also demonstrated that PPOSS-IDA-Cu²⁺ had potential application in removing highly abundant proteins in proteomic analysis, and can be combined with other techniques to get more information on low abundant proteins.
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