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Citation: WU Hongxing, ZHENG Aini, SU Caoyin, YU Nuojun, LI Ning. Simultaneous separation of seven water-soluble vitamins with microemulsion liquid chromatography[J]. Chinese Journal of Chromatography, ;2017, 35(4): 439-444. doi: 10.3724/SP.J.1123.2016.09037 shu

Simultaneous separation of seven water-soluble vitamins with microemulsion liquid chromatography

Guangdong Pharmaceutical University, Guangzhou 510006, China

Received Date: 19 September 2016

Fund Project: National Natural Science Foundation of China (No. 81173525).

A novel method was developed for the separation of seven water-soluble vitamins (V_B1, V_B2, V_B6, V_B12, V_C, folic acid and nicotinamide) with microemulsion liquid chromatography. The impacts of following factors on the separation selectivity were investigated:the type of surfactant, the type of oil phase, the type of co-surfactant, the pH of mobile phase and the column temperature. The optimal conditions for the separation of the seven water-soluble vitamins were as follows:Agilent TC C18 (250 mm×4.6 mm, 5 μm) was used at 30℃; the microemulsion mobile phase was sodium dodecyl sulfate (SDS)/polyoxyethylene lauryl ether (Brij35)/n-butanol/ethyl acetate/water (2:60:66:80:864, mass ratio). Baseline separation of the seven water-soluble vitamins was achieved within 20 min with a flow rate of 0.5 mL/min and the detection wavelength of 254 nm. The calibration curve showed a good linearity with the correlation coefficients above 0.9991 in the linear range from 4 to 36 mg/L. The mean recoveries of V_B1, V_B2, V_B6, V_C and nicotinamide were 93.9%-102.9% at different spiked levels. The optimized and validated method can be used for simultaneous determination of water-soluble vitamins in food and pharmaceuticals.
- microemulsion liquid chromatography (MELC),
- water-soluble vitamins,
- separation selectivity
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