Citation: ZHAO Bo, DENG Meilin, YANG Xiaoshan, DUAN Yunpeng, WU Xiaoqin, TU Dawei. Determination of nine N-nitrosamines in animal derived foods by dispersive solid-phase extraction-gas chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, ;2016, 34(10): 998-1004. doi: 10.3724/SP.J.1123.2016.07012 shu

Determination of nine N-nitrosamines in animal derived foods by dispersive solid-phase extraction-gas chromatography-tandem mass spectrometry

Chongqing Institute for Food and Drug Control; Chongqing Engineering Research Center for Pharmaceutical Process and Quality Control, Chongqing 401121, China

Received Date: 10 July 2016

Fund Project: Support Program of Chongqing Institute for Food and Drug Control (No. SNKY-2015-07).

A method has been developed and validated for the determination of nine N-nitrosamines in animal derived food using dispersive solid-phase extraction (dSPE) coupled with isotope dilution-gas chromatography-tandem mass spectrometry (GC-MS/MS). The samples were extracted by acetonitrile. The extracts were purified using mixed adsorbent by dSPE and then concentrated. The analytes were separated on a DB-WAX polar capillary column, and ionized by EI source. The data were collected by multiple reaction monitoring (MRM) mode, followed by qualitative screening and quantitative analysis. The correlation coefficients of linear calibration curves were over 0.99 in the corresponding linear ranges. Four kinds of typical animal derived foods were spiked at three levels. The average recoveries of the nine N-nitrosamines ranged from 62.5% to 118% with RSDs of 2.11%-25.6%. The limits of detection (LODs, S/N=3) were 0.02-0.31 μg/kg. The method is of low cost, sensitive, reliable, and suitable for the determination of nine N-nitrosamines in animal derived foods.
- dispersive solid-phase extraction (dSPE),
- isotope dilution,
- gas chromatography-tandem mass spectrometry (GC-MS/MS),
- N-nitrosamines,
- animal derived foods
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