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Citation: GAO Xiaodi, ZHANG Weibing. Preparation and glycopeptide enrichment application of magnetic nanoparticles functionized by poly- (allyl chloride)-glucose based on click reaction[J]. Chinese Journal of Chromatography, ;2016, 34(8): 745-751. doi: 10.3724/SP.J.1123.2016.05027 shu

Preparation and glycopeptide enrichment application of magnetic nanoparticles functionized by poly- (allyl chloride)-glucose based on click reaction

GAO Xiaodi ,
ZHANG Weibing ^,

1.
Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China

Corresponding author: ZHANG Weibing,
Received Date: 28 May 2016

Fund Project:

The abundance of glycoproteins and the glycans attached are usually low in body. In order to analyze them accurately and eliminate the interference of non-glycoproteins and non-glycopeptides, methods of enrichment are needed. A kind of novel magnetic nanoparticles based on click reaction and multiple-functional-site poly-(allyl chloride) structure is designed and prepared. The related hydrophilic enrichment method for glycopeptide is developed. Totally 32 glycopeptide signals were detected from human immunoglobulin G trypsin enzymolysis under the optimized conditions. The method can reach a limit of detection of 10 fmol on human immunoglobulin G trypsin enzymolysis, with an adsorption capacity of 100 mg of 1 g particles.
- click reaction,
- magnetic enrichment nanomaterials,
- glycopeptide,
- human immunoglobulin G
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