Citation: WU Xuejing, JIN Jie, YAN Wei, XIAO Hua, FAN Liuyin, CAO Chengxi. Numerical computation and experimental verification of a derivatized moving reaction boundary originally created with formic buffer and sodium hydroxide[J]. Chinese Journal of Chromatography, ;2016, 34(8): 801-810. doi: 10.3724/SP.J.1123.2016.04029 shu

Numerical computation and experimental verification of a derivatized moving reaction boundary originally created with formic buffer and sodium hydroxide

1.
Laboratory of Analytical Biochemistry & Bioseparation, School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China

Corresponding author: FAN Liuyin, CAO Chengxi,
Received Date: 19 April 2016

A model of derivatized moving reaction boundary (MRB) is formulated from original MRB formed with formic buffer (phase α) and sodium hydroxide (phase γ). The model shows the formation of a new phase (phase β) with high pH value originated from phase γ, derivatized MRB created with phases α and β, as well as stationary boundary formed between phases β and γ. To demonstrate the validity of this model, the theoretical and numerical procedures are advanced. At the same time, the experimental procedures are also developed relied on a capillary electrophoresis (CE) and a home-made apparatus. There are evident systemic errors and low agreements between the experimental results and theoretical calculation with the original model of MRB developed in our previous papers. However, there are much high agreements between the experiments and theoretical computation relied on the model of derivatized MRB developed herein. The model of derivatized MRB together with the relative theoretical and experimental procedures holds evident significance for the design of the new separation and compressive techniques of samples used widely in electrophoresis including CE.
- electromigration,
- electrophoresis,
- numerical computation,
- moving reaction boundary (MRB),
- stacking
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