Citation: DONG Yu, YU Zhongming, LI Hongyu, ZHAO Lisha, SHOU Dan. Rat urinary metabolomics analysis of intervention of Phellinus igniarius based on ultra high performance liquid chromatography with high- definition mass spectrometry[J]. Chinese Journal of Chromatography, ;2016, 34(8): 811-816. doi: 10.3724/SP.J.1123.2016.04009 shu

Rat urinary metabolomics analysis of intervention of Phellinus igniarius based on ultra high performance liquid chromatography with high- definition mass spectrometry

DONG Yu ¹ ,
YU Zhongming ¹ ,
LI Hongyu ¹ ,
ZHAO Lisha ² ,
SHOU Dan ^1,,

1.
1. Key Laboratory of Research and Development of Traditional Chinese Medicine, Zhejiang Academy of Traditional Chinese Medicine, Hangzhou 310007, China;
2.
2. College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou 310053, China

Corresponding author: SHOU Dan,
Received Date: 6 April 2016

Fund Project:

To investigate the influence of oral administration of decoction of Phellinus igniarius on the body, this study utilized ultra high performance liquid chromatography with high-definition mass spectrometry (UPLC-HDMS) technology to detect the change of metabolites in rat urine after oral decoction of Phellinus igniarius. Orthogonal partial least squares discriminant analysis (OPLS-DA) was adapted to analyze the metabolites of rat urine in the control group and dosed group, then the potential biomarkers were screened out. Finally the related metabolic pathways were analyzed by MetaboAnalyst 3.0 website. The results showed that urinary metabolites in the two groups were well separated at the 28th day, and 10 biomarkers were found and identified. After the oral administration of decoction of Phellinus igniarius, it mainly impacted the cysteine and methionine metabolism, arginine and proline metabolism, purine metabolism pathways. These results of the study would lay a foundation for further research on the pharmacodynamic mechanism of Phellinus igniarius.
- ultra high performance liquid chromatography (UPLC),
- high-definition mass spectrometry (HDMS),
- metabolomics,
- pattern recognition analysis,
- Phellinus igniarius,
- rat urine
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